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West Point/11 August 2011
From 2011.igem.org
Click on a date to see a summary of labwork done on that day (red pages are the only ones that have an entry). Up-to-date to 5September11. Best viewed with Mozilla Firefox. Props to the 2008 Edinburgh team, we used quite a bit of their code for this.
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09AUGUST11
Develop procedure for making buffer solutions for AP and X-phos; collect materials
o BCIP Buffer
0.1 M Tris
0.1M NaCl
5 mM MgCl2
Add 0.1 M HCl until pH reaches 9.5
o A.P. Buffer
10 mM Tris
5 mM MgCl2
0.2 mM ZnCl2
Add 0.1 M HCl until pH reaches 8.0
