Team:Lyon-INSA-ENS/Realisation/Collection

From 2011.igem.org

(Difference between revisions)

Latest revision as of 17:05, 19 September 2011

Plasmids collection
Collection reference	Description	Resistance(s)
pIG1	phaCAB	-
pIG2	rcn-curli cloned in pUC57 plasmid	Amp
pIG3	Pfort (18A: BBa_J23119)	Amp
pIG4	part Bba_K342000 = Pcurli	Cm
pIG5	enhanced yellow fluorescent protein derived from A. victoria GFP (24E: BBa_E0030)	Amp+K
pIG6	pUC18	Amp
pIG7	pUC18	Amp
pIG8	Weak RBS (5L: Bba_J61101)	Amp
pIG9	GFP with LVA tag (2L: BBa_K145015)	Amp
pIG10	RBS-YFP (22M: BBa_I15017)	Amp+K
pIG11	OmpR234 (BBa_K342003)	Cm
pIG13	RBS-GFP-LVA (BBa_K206009)	Cm
pIG14	Strong RBS (2M: BBa_B0034)	Amp
pIG15	NiCoT	Amp
pIG16	18A-OmpR234 (pIG3+pIG11)	Amp
pIG17	Strong RBS-YFP (pIG14+pIG10) (2M24E)	Amp
pIG18	RBSfort-GFP-Tet (pIG14+pIG9) (2M2L)	T
pIG19	RBSfort-GFP-Kan (pIG14+pIG9) (2M2L)	K
pIG20	Prcn-csg extracted in pUC57 ()	Amp
pIG21	Backbone A = pSB1A3	Amp
pIG22	Backbone K = pSB1K3	K
pIG23	Backbone T = pSB1T3	T
pIG24	Backbone C = pSB1C3	Cm
pIG25	Synthetic part cloned in pSB1C3	Cm
pIG26	part Prcn (= rcnA promoter)	Cm
pIG27	part Prcn (= rcnA promoter)	Cm
pIG28	part Pcurli-RBS-GFP(LVA) (pIG4+pIG18)	Cm
pIG29	part CsgBA	Amp
pIG30	pIG25	-
pIG31	pKK-OmpR234 (p56 collection biofilm)	Amp
pIG32	pPROBE OT'-Prcn-GFP (p157 collection biofilm)	Spc
pIG33	PrcnA-OmpR234	Cm
pIG34	PrcnA-OmpR234	K
pIG35	= pIG25	Cm

Strains collection
Collection reference	Description	Phenotype
S5		Amp resistant
S6	NM522/pIG26	Cm resistant
S7	MC4100	streptomycin resistant
S8	MG1655	-
S9	NM522/pIG27	Cm resistant
S10	NM522/pIG26	Cm resistant
S11	NM522	-
S12	DH5α	nalidixic acid resistant
S13	NMYO	Kan resistant
S14	NM522 + pTNiCo	Amp resistant, pTNiCo : NiCo transporter from Novosphingobium aromaticivorans inserted in pUC19
S15	PHL818	Tet resistant, sticky
S16	PHL1273	Tet resistant, Kan resistant
S17	NM522/pIG25	Cm resistant
S18	NM522/pIG2	Amp resistant
S19=S20	NM522/pIG16	Amp resistant
S21	NM522/pIG6	AmpR
S22	NM522/pIG33	-
S23	PHL1414/pIG2	CmR (genomic), AmpR (plasmidic). Constitutively fluorescent
S24	PHL1414/pIG6	CmR (genomic), AmpR (plasmidic). Constitutively fluorescent
S25	MC4100/piG6	AmpR
S26	MC4100/piG2	AmpR
S27	MC4100/pIG27	CmR
S28	MC4100/pIG16	AmpR
S29	PHL1414	CmR (genomic), constitutively fluorescent
S30	PHL1414/pIG3	CmR (genomic), AmpR (plasmidic). Constitutively fluorescent
S31	PHL1414/pIG16	CmR (genomic), AmpR (plasmidic). Constitutively fluorescent
S32	MC4100/pIG34	KanR
S33	MC4100/pIG33	CmR

@@ Line 3: / Line 3: @@
 {{INSA-Lyon/styletestaurelie}}
 {{Lyon-INSA-ENS/menuhorizontal}}
-{{Lyon-INSA-ENS/menuNotebookCollection}}
+<!--{{Lyon-INSA-ENS/menuNotebookCollection}}-->
+{{Lyon-INSA-ENS/menuNotebookVertical|Collection = actif}}
 <html>
+<div style="float : left; margin-top : -10px; margin-left : -200px">
+   <a href="https://2011.igem.org/Main_Page" >
+       <img src="https://static.igem.org/mediawiki/2011/6/67/Team_INSA-Lyon_IGEM_Home.png" title="iGEM's main page" />
+   </a>
+</div>
 <style type="text/css">
@@ Line 172: / Line 179: @@
 </tr>
 <tr>
-<td>S5</td> <td>NM522/pGem-T with rcnR inserted</td> <td> Amp resistant </td>
+<td>S5</td> <td></td> <td> Amp resistant </td>
 </tr>
 <tr>
-<td>S6</td> <td>NM522/pIG26</td> <td> </td>
+<td>S6</td> <td>NM522/pIG26</td> <td> Cm resistant</td>
 </tr>
 <tr>
-<td>S7</td> <td>MC4100</td> <td> </td>
+<td>S7</td> <td>MC4100</td> <td>streptomycin resistant </td>
 </tr>
 <tr>
-<td>S8</td> <td>MG1655</td> <td> </td>
+<td>S8</td> <td>MG1655</td> <td> - </td>
 </tr>
 <tr>
-<td>S9</td> <td>NM522/pIG27</td> <td> </td>
+<td>S9</td> <td>NM522/pIG27</td> <td>Cm resistant </td>
 </tr>
 <tr>
-<td>S10</td> <td>NM522/pIG26</td> <td> </td>
+<td>S10</td> <td>NM522/pIG26</td> <td>Cm resistant </td>
 </tr>
 <tr>
-<td>S11</td> <td>NM522</td> <td> </td>
+<td>S11</td> <td>NM522</td> <td> - </td>
 </tr>
 <tr>
-<td>S12</td> <td>DH5α</td> <td> </td>
+<td>S12</td> <td>DH5α</td> <td>nalidixic acid resistant  </td>
 </tr>
 <tr>
-<td>S13</td> <td>NMYO</td> <td> </td>
+<td>S13</td> <td>NMYO</td> <td>Kan resistant </td>
 </tr>
 <tr>
-<td>S14</td> <td>NM522 + pTNiCo</td> <td> </td>
+<td>S14</td> <td>NM522 + pTNiCo</td> <td>Amp resistant, pTNiCo : NiCo transporter from Novosphingobium aromaticivorans inserted in pUC19</td>
 </tr>
 <tr>
-<td>S15</td> <td>MG1655 malT::Tn10 ompR234</td> <td> </td>
+<td>S15</td> <td>PHL818</td> <td>Tet resistant, sticky</td>
 </tr>
 <tr>
-<td>S16</td> <td></td> <td> </td>
+<td>S16</td> <td>PHL1273</td> <td>Tet resistant, Kan resistant</td>
 </tr>
 <tr>
-<td>S17</td> <td></td> <td> </td>
+<td>S17</td> <td>NM522/pIG25</td> <td>Cm resistant</td>
 </tr>
 <tr>
-<td>S18</td> <td></td> <td> </td>
+<td>S18</td> <td>NM522/pIG2</td> <td>Amp resistant</td>
 </tr>
 <tr>
-<td>S19</td> <td></td> <td> </td>
+<td>S19=S20</td> <td>NM522/pIG16 </td> <td>Amp resistant</td>
+</tr>
+<tr>
+<td>S21</td> <td>NM522/pIG6</td> <td>AmpR</td>
 </tr>
 <tr>
-<td>S20</td> <td></td> <td> </td>
+<td>S22</td> <td>NM522/pIG33</td> <td> - </td>
+</tr>
+<tr>
+<td>S23</td> <td>PHL1414/pIG2</td> <td> CmR (genomic), AmpR (plasmidic). Constitutively fluorescent</td>
+</tr>
+<tr>
+<td>S24</td> <td>PHL1414/pIG6</td> <td>CmR (genomic), AmpR (plasmidic). Constitutively fluorescent</td>
 </tr>
 <tr>
-<td>S21</td> <td></td> <td> </td>
+<td>S25</td> <td>MC4100/piG6</td> <td>AmpR</td>
 </tr>
 <tr>
-<td>S22</td> <td></td> <td> </td>
+<td>S26</td> <td>MC4100/piG2</td> <td>AmpR</td>
 </tr>
 <tr>
-<td>S23</td> <td></td> <td> </td>
+<td>S27</td> <td>MC4100/pIG27</td> <td>CmR</td>
 </tr>
 <tr>
-<td>S24</td> <td></td> <td> </td>
+<td>S28</td> <td>MC4100/pIG16</td> <td>AmpR</td>
 </tr>
 <tr>
-<td>S25</td> <td></td> <td> </td>
+<td>S29</td> <td>PHL1414</td> <td>CmR (genomic), constitutively fluorescent</td>
 </tr>
 <tr>
-<td>S26</td> <td></td> <td> </td>
+<td>S30</td> <td>PHL1414/pIG3</td> <td>CmR (genomic), AmpR (plasmidic). Constitutively fluorescent</td>
 </tr>
 <tr>
-<td>S27</td> <td></td> <td> </td>
+<td>S31</td> <td>PHL1414/pIG16 </td> <td>CmR (genomic), AmpR (plasmidic). Constitutively fluorescent</td>
 </tr>
 <tr>
-<td>S28</td> <td></td> <td> </td>
+<td>S32</td> <td>MC4100/pIG34</td> <td>KanR</td>
 </tr>
 <tr>
-<td>S29</td> <td></td> <td> </td>
+<td>S33</td> <td>MC4100/pIG33</td> <td>CmR</td>
 </tr>
 </table>