Team:Paris Liliane Bettencourt/Notebook/2011/09/07/

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(Created page with "{{:Team:Paris_Bettencourt/tpl_test}} == Cyrille == === Miniprep and sequencing === Miniprep of: * pVeg-YFP TetR in pHM3 -> Sequence ok * TetO pVeg-YFP TetR in pHM3 -> Sequenc...")
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* YFP TetR BB -> SpeI site are not there in the 2 clones sequenced
* YFP TetR BB -> SpeI site are not there in the 2 clones sequenced
* ComS -> Ok on the two clones sequenced
* ComS -> Ok on the two clones sequenced
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== Hovannes-Baptiste ==
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=== Preparation of slides ===
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Dilution of overnight cultures : PY79 (gfp-) and PY79 (gfp+) . <br>
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We waited to an OD of 0.4 (600 nm).
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Two well slides : 1-control (PY79 only)  2-Mix (both strains)
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<br>
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=== Observation  ===
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-37°C Microscopy-<br>
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 +
We observed the plate with TRANS and YFP-filter settings on the Nikon microscope. We quickly saw that our PY79 + S12 gene was not correct since we found only one cell producing GFP. We will retry the experiment tomorrow with the other -20°C glycerol for this strain, hoping it is not contaminated.
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[[File:0907_trans_PY79_PY79S12.jpg|500px|thumb|center|TRANS at t=0min]]
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[[File:0907_fluo_PY79_PY79S12.jpg|500px|thumb|center|GFP at t=0min]]
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We followed the plate a few hours nonetheless but no result was visible (the fluorescent cell divided only 2 times).
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Latest revision as of 20:18, 17 September 2011

Team IGEM Paris 2011

Contents

Cyrille

Miniprep and sequencing

Miniprep of:

  • pVeg-YFP TetR in pHM3 -> Sequence ok
  • TetO pVeg-YFP TetR in pHM3 -> Sequence shows that only TetO was there
  • YFP TetR BB -> SpeI site are not there in the 2 clones sequenced
  • ComS -> Ok on the two clones sequenced

Hovannes-Baptiste

Preparation of slides

Dilution of overnight cultures : PY79 (gfp-) and PY79 (gfp+) .


We waited to an OD of 0.4 (600 nm).

Two well slides : 1-control (PY79 only) 2-Mix (both strains)

Observation

-37°C Microscopy-

We observed the plate with TRANS and YFP-filter settings on the Nikon microscope. We quickly saw that our PY79 + S12 gene was not correct since we found only one cell producing GFP. We will retry the experiment tomorrow with the other -20°C glycerol for this strain, hoping it is not contaminated.

TRANS at t=0min
GFP at t=0min


We followed the plate a few hours nonetheless but no result was visible (the fluorescent cell divided only 2 times).


Retrieved from "http://2011.igem.org/Team:Paris_Liliane_Bettencourt/Notebook/2011/09/07/"