Team:Imperial College London/Sequence

From 2011.igem.org

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<body>
<body>
<h1>Sequencing Results</h1>
<h1>Sequencing Results</h1>
-
</body>
+
<h2>Vector 7 forward sequencing result</h2>
 +
 
 +
<p> BBa_K316001 (7)_premix -- 19..973 of sequence </p>
 +
 
 +
<p>TATAAAAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCT
 +
AAGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGAATTTTGTCAAAATAATTTTATTG
 +
ACAACGTCTTATTAACGTTGATATAATTTAAATTTTATTTGACAAAAATGGGCTCGTGTT
 +
GTACAATAAATGTTACTAGTAGCGGCCGCTGCAGTCCGGCAAAAAAACGGCAAGGTGTCA
 +
CCACCCTGCCCTTTTTCTTTAAAACCGAAAAGATTACTTCGCGTTATGCAGGCTTCCTCG
 +
CTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAG
 +
GCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAA
 +
GGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCACAGGCTC
 +
CGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACA
 +
GGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCG
 +
ACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCT
 +
CATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGT
 +
GTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAG
 +
TCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGGTAACAGGATTAG
 +
CAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTA
 +
CACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGA</p>
 +
 
 +
<h2>Vector 8 forward sequencing result</h2>
 +
 
 +
<p>BBa_K316005 (8)_premix -- 17..635 of sequence</p>
 +
<p>TAGAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTAAG
 +
GATGATTTCTGGAATTCGCGGCCGCTTCTAGAGAATTTTGTCAAAATAATTTTATTGACA
 +
ACGTCTTATTAACGTTGATATAATTTAAATTTTTTTTGACAAAAATGGGCTCGTGTTGTA
 +
CAATAAATGTTACTAGAGCTCATGAACTATGAAGAGGTGACGTCATGAACAAAGGTGTAA
 +
TGCGACCGGGCCATGTGCAGCTGCGTGTACTGGACATGAGCAAGGCCCTGGAACACTACG
 +
TCGAGTTGCTGGGCCTGATCGAGATGGACCGTGACGACCAGGGCCGTGTCTATCTGAAGG
 +
CTTGGACCGAAGTGGATAAGTTTTCCCTGGTGCTACGCGAGGCTGACGAGCCGGGCATGG
 +
ATTTTATGGGTTTCAAGGTTGTGGATGAGGATGCTCTCCGGCAACTGGAGCGGGATCTGA
 +
TGGCATATGGCTGTGCCGTTGAGCAGCTACCCGCAGGTGAACTGAACAGTTGTGGCCGGC
 +
GCGTGCGCTTCCAGGCCCCCTCCGGGCATCACTTCGAGTTGTATGCAGACAAGGAATATA
 +
CTGGAAAGTGGGGTTTGAA</p>
 +
 
 +
<h2>Vector 6 forward sequencing result</h2>
 +
 
 +
<p>BBa_K398500 (6)_premix -- 17..960 of sequence</p>
 +
<p>TATAAAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTA
 +
AGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGTTGACGGCTAGCTCAGTCCTAGGTA
 +
CAGTGCTAGCTTCTAGAGAAAGAGGGGACAAACTAGATGCGTAAAGGAGAAGAACTTTTC
 +
ACTGGAGTTGTCCCAATTCTTGTTGAATTAGATGGTGATGTTAATGGGCACAAATTTTCT
 +
GTCAGTGGAGAGGGTGAAGGTGATGCAACATACGGAAAACTTACCCTTAAATTTATTTGC
 +
ACTACTGGAAAACTACCTGTTCCATGGCCAACACTTGTCACTACTTTCGGTTATGGTGTT
 +
CAATGCTTTGCGAGATACCCAGATCATATGAAACAGCATGACTTTTTCAAGAGTGCCATG
 +
CCCGAAGGTTATGTACAGGAAAGAACTATATTTTTCAAAGATGACGGGAACTACAAGACA
 +
CGTGCTGAAGTCAAGTTTGAAGGTGATACCCTTGTTAATAGAATCGAGTTAAAAGGTATT
 +
GATTTTAAAGAAGATGGAAACATTCTTGGACACAAATTGGAATACAACTATAACTCACAC
 +
AATGTATACATCATGGCAGACAAACAAAAGAATGGAATCAAAGTTAACTTCAAAATTAGA
 +
CACAACATTGAAGATGGAAGCGTTCAACTAGCAGACCATTATCAACAAAATACTCCAATT
 +
GGCGATGGCCCTGTCCTTTTACCAGACAACCATTACCTGTCCACACAATCTGCCCTTTCG
 +
AAAGATCCCAACGAAAAGAGAGACCACATGGTCCTTCTTGAGTTTGTAACAGCTGCTGGG
 +
ATTACACATGGCATGGATGAACTATACAAATAATAATACTAGAGCCAGGCATCAAATAAA
 +
ACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTT</p>
 +
 
 +
<h2>?</h2>
 +
 
 +
<p>PSVi34-1_premix -- 19..951 of sequence</p>
 +
<p>CATGAATGTCTTTGTACGAAACTCATTACCCGCATTAATATTTTTTTTCTTTTTAAAGCT
 +
CAGTTGACCCTTTCTCATTCCCTTCTTAAAACAACTGTGTGATCCTTGAGAAAAGATAAA
 +
TTACATACACAACATAAACCCAACTACTAGTTATTAAAATGTCTAAAGGTGAAGAATTAT
 +
TCACTGGTGTTGTCCCAATTTTGGTTGAATTAGATGGTGATGTTAATGGTCACAAATTTT
 +
CTGTCTCCGGTGAAGGTGAAGGTGATGCTACTTACGGTAAATTGACCTTAAAATTTATTT
 +
GTACTACTGGTAAATTGCCAGTTCCATGGCCAACCTTAGTCACTACTTTCGGTTATGGTG
 +
TTCAATGTTTTGCGAGATACCCAGATCATATGAAACAACATGACTTTTTCAAGTCTGCCA
 +
TGCCAGAAGGTTATGTTCAAGAAAGAACTATTTTTTTCAAAGATGACGGTAACTACAAGA
 +
CCAGAGCTGAAGTCAAGTTTGAAGGTGATACCTTAGTTAATAGAATCGAATTAAAAGGTA
 +
TTGATTTTAAAGAAGATGGTAACATTTTAGGTCACAAATTGGAATACAACTATAACTCTC
 +
ACAATGTTTACATCATGGCTGACAAACAAAAGAATGGTATCAAAGTTAACTTCAAAATTA
 +
GACACAACATTGAAGATGGTTCTGTTCAATTAGCTGACCATTATCAACAAAATACTCCAA
 +
TTGGTGATGGTCCAGTCTTGTTACCAGACAACCATTACTTATCCACTCAATCTGCCTTAT
 +
CCAAAGATCCAAACGAAAAGAGAGACCACATGGTCTTGTTAGAATTTGTTACTGCTGCTG
 +
GTATTACCCATGGTATGGATGAATTGTACAAATAACTCGAGTAAGCTTGGTACCGCGGCT
 +
AGCTAAGATCCGCTCTAACCGAAAAGGAAGGAG</p>
 +
 
 +
<h2>?</h2>
 +
 
 +
<p>PSVi34-2_premix -- 21..949 of sequence</p>
 +
<p>ATGAATGTCTTTGTACGAAACTCATTACCCGCATTAATATTTTTTTTCTTTTTAAAGCTC
 +
AGTTGACCCTTTCTCATTCCCTTCTTAAAACAACTGTGTGATCCTTGAGAAAAGATAAAT
 +
TACATACACAACATAAACCCAACTACTAGTTATTAAAATGTCTAAAGGTGAAGAATTATT
 +
CACTGGTGTTGTCCCAATTTTGGTTGAATTAGATGGTGATGTTAATGGTCACAAATTTTC
 +
TGTCTCCGGTGAAGGTGAAGGTGATGCTACTTACGGTAAATTGACCTTAAAATTTATTTG
 +
TACTACTGGTAAATTGCCAGTTCCATGGCCAACCTTAGTCACTACTTTCGGTTATGGTGT
 +
TCAATGTTTTGCGAGATACCCAGATCATATGAAACAACATGACTTTTTCAAGTCTGCCAT
 +
GCCAGAAGGTTATGTTCAAGAAAGAACTATTTTTTTCAAAGATGACGGTAACTACAAGAC
 +
CAGAGCTGAAGTCAAGTTTGAAGGTGATACCTTAGTTAATAGAATCGAATTAAAAGGTAT
 +
TGATTTTAAAGAAGATGGTAACATTTTAGGTCACAAATTGGAATACAACTATAACTCTCA
 +
CAATGTTTACATCATGGCTGACAAACAAAAGAATGGTATCAAAGTTAACTTCAAAATTAG
 +
ACACAACATTGAAGATGGTTCTGTTCAATTAGCTGACCATTATCAACAAAATACTCCAAT
 +
TGGTGATGGTCCAGTCTTGTTACCAGACAACCATTACTTATCCACTCAATCTGCCTTATC
 +
CAAAGATCCAAACGAAAAGAGAGACCACATGGTCTTGTTAGAATTTGTTACTGCTGCTGG
 +
TATTACCCATGGTATGGATGAATTGTACAAATAACTCGAGTAAGCTTGGTACCGCGGCTA
 +
GCTAAGATCCGCTCTAACCGAAAAGGAAG</p>
 +
 
 +
<h2>Sequence results for J23113 ligation attempt into RFP. Failed.</h2>
 +
 
 +
<p> 52.E_premix -- 14..957 of sequence</p>
 +
<p>ATAAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTAAG
 +
GATGATTTCTGGAATTCGCGGCCGCTTCTAGAGAAAGAGGAGAAATACTAGATGGCTTCC
 +
TCCGAAGACGTTATCAAAGAGTTCATGCGTTTCAAAGTTCGTATGGAAGGTTCCGTTAAC
 +
GGTCACGAGTTCGAAATCGAAGGTGAAGGTGAAGGTCGTCCGTACGAAGGTACCCAGACC
 +
GCTAAACTGAAAGTTACCAAAGGTGGTCCGCTGCCGTTCGCTTGGGACATCCTGTCCCCG
 +
CAGTTCCAGTACGGTTCCAAAGCTTACGTTAAACACCCGGCTGACATCCCGGACTACCTG
 +
AAACTGTCCTTCCCGGAAGGTTTCAAATGGGAACGTGTTATGAACTTCGAAGACGGTGGT
 +
GTTGTTACCGTTACCCAGGACTCCTCCCTGCAAGACGGTGAGTTCATCTACAAAGTTAAA
 +
CTGCGTGGTACCAACTTCCCGTCCGACGGTCCGGTTATGCAGAAAAAAACCATGGGTTGG
 +
GAAGCTTCCACCGAACGTATGTACCCGGAAGACGGTGCTCTGAAAGGTGAAATCAAAATG
 +
CGTCTGAAACTGAAAGACGGTGGTCACTACGACGCTGAAGTTAAAACCACCTACATGGCT
 +
AAAAAACCGGTTCAGCTGCCGGGTGCTTACAAAACCGACATCAAACTGGACATCACCTCC
 +
CACAACGAAGACTACACCATCGTTGAACAGTACGAACGTGCTGAAGGTCGTCACTCCACC
 +
GGTGCTTAATAACGCTGATAGTGCTAGTGTAGATCGCTACTAGAGATTAAAGAGGAGAAA
 +
TACTAGTAGCGGCCGCTGCAGGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGG
 +
CTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTT</p>
 +
 
 +
<h2>Sequence results for possible auxin producing colony</h2>
 +
 
 +
<p>53_AUX1 -- 13..914 of sequence</p>
 +
<p>GCATCTGATTTTCCAATAGTATGTTAGAAATCTTTCGTGTTGTGATGACGAATTGTGATG
 +
AGCACCAGCATCTGATTGTTGGTGGCGTACAGCAAGTGCCTGTAGGCCTGTGGTCGCACG
 +
TGCCTGAACGCTGCGCACATTGGCCAAAGGGTACGTCTCTTTCCTCACTTCATCGGGGTG
 +
CCCCGCGCCCAGGTGTCAAACGTATTGCACGTGCTGAAGATGGTTCATTTACGGTTACAG
 +
ATAATTGGGGCGATACACGTCAATACGCTGCCGTTTTGACAACCTGTCAATCGTGGCTGC
 +
TTACAACGCAGATTGAATGTGAGGAATCTTTGTTTTCACAGAAGATGTGGATGGCGCTGG
 +
ATCGCACGCGTTACATGCAGTCTTCTAAAACTTTTGTGATGGTTGATCGGCCGTTTTGGA
 +
AGGACAAAGATCCGGAAACGGGTCGCGATCTTATGTCCATGACCCTCACCGATCGCTTAA
 +
CGCGCGGCACATACTTATTTGATAATGGCGACGACAAGCCAGGCGTTATTTGCCTGAGTT
 +
ACTCATGGATGAGTGACGCTTTAAAAATGTTACCGCAACCGATCGAGAAACGCGTCAAAT
 +
TAGCTTTAGATGCCCTTAAAAAGATTTACCCGAAAGTGGATATCGCCGCACGTATTATTG
 +
GTGACCCGATTACTGTGAGTTGGGAAGCGGATCCGCATTTCCTGGGGGCATTTAAAGGCG
 +
CGTTACCGGGCCATTACCGTTACAACCAGCGCATGTACGCGCATTTTATGCAGCAGACAT
 +
GCCGTCAGAACAGCGTGGCATGTTTATTGCCGGTGATGATGTATCTTGGACACCGGCATG
 +
GGTTGAGGGAGCAGTACAAACGTCATTGAACGCGGTGTGGGGCATCATGAACCATTTCGG
 +
AG</p>
 +
 
 +
<p>53_AUX2 -- 16..895 of sequence</p>
 +
<p>GGACAGTACAACGTCATTGAACGCGGTGTGGGGCATCATGAACCATTTCGGAGGGAAAAC
 +
CCACGCAGAGAATCCGGGTCCTGGCGACGTTTTCCATGAGATTGGACCGATCGCGCTGGG
 +
GGACTAATAATCGCTGGGACGCCCGCCCCTAGAAAAGGAGGACTAAAAATGCGCGAAATG
 +
ATTACGCTTGAATCTCTGTGCCGCGCATTGGCAGACGAACAGATTGCCGCAGAAGAATTG
 +
CGCGAGCGCGCGCTGGATACAGAGGCTCGCTTAACACTGTTAAATTGCTTCATTCGCGAA
 +
GGCGACGCCGTAAGCCAATTCGGTGAAGCGGATCAGGCGCGTAAAGGAACTTCACTTTGG
 +
GGGGTGCCTGTGAGTTTTAAGGATAACATCTGTGTTCGCGGGCTGCCACTGACGGCGGGT
 +
ACCCGCGGCATGAGCGGCTTTATTGCCGATCAGGATGCAGCAATCGTGAGCCAGTTAAAA
 +
GCATTGGGAGCTGTGGTCGCGGGCAAAAATAACATGCATGAGCTGTCATTTGGCGTCACG
 +
AGCATCAATCCACATTGGGGCGCGGTTGGAAACCCGGTGGCTCCAGGATATTGCGCCGGA
 +
GGTTCTAGCGGAGGTTCCGCTGCGGCGGTCGCATCTGGAATTGTCCCGCTCAGCGTCGGG
 +
ACGGACACAGGCGGCTCAATCCGTATTCCGGCGGCATTTTGCGGCATTACGGGTTTTCGC
 +
CCTACTACAGGTCGCTTGAGTACGGCGGGGATCATTCCGGTCAGCCACACGAAAGGATTG
 +
TGTCGGCCTCCTGACGCGCACAGCTGGCGACGCTGAATTTGTGTATGGGCTGCTGTCAGA
 +
AAACAGCAGAGTTTCCCTCTTAATCGCACGGGACCTTGCG</p>
 +
 
 +
<p>53_AUX3 -- 15..793 of sequence</p>
 +
<p>GATGATGTGTCGGCCTCCTGACGCGCACAGCTGGCGACGCTGAATTTGTGTATGGGCTGC
 +
TGTCAGGAAAACAGCAGAGTTTCCCTCTTAATCGCACGGGACCTTGCCGCATCGGCCTGC
 +
CGGTGAGCATGTGGTCTGACTTAGATGGCGAAGTAGAACGTGCCTGTATCAATGCGCTGA
 +
GCCTCTTACGCAAAACGGGCTTTGAATTTGTTGAGATCGACGATGCGGACATTGTGGAAT
 +
TGAACCAGACATTAACCTTCACGGTGCCGCTGTACGAATTTTTCGCGGATTTTGCTCAAT
 +
CCTTGTTGTCCTTAGGTTGGAAACATGGCATCCATCATATTTTTGCACAAGTGGATGATG
 +
CAAATGTGAAAGGCATTATTAATCACCATCTGGGAGAGGGCGCGATTAAGCCGGCACATT
 +
ATCTGAGTTCTCTCCAGAATGGAGAGCTGCTGAAACGCAAGATGGATGAGCTTTTTGCGC
 +
GCCATCACATTAAACTTTTAGGGTATCCGACGGTCCCGTGTCGCGTGCCTCATCTGGACC
 +
ATGCAGATCGTCCGGAATTTTTTAGCCAGGCCATCCGTAACACAGACCTCGCAAGCAACG
 +
CGATGTTACCAAGCATTACAATTCCGGTCGGTCCGGAAGGCCGCCTGCCGGTCGGCCTTA
 +
GTTTTGATGCGCCTCGTGCTCGTGATGCGTTTCTCTTAAGCAATGTTAGTTTGATCGAAA
 +
AAGTGTTAAAAGGCTAATAATACTAGTAGCGGCCGCTGCAGTCCGGCAAAAAAGGGCAA</p>
 +
 
 +
<p>53_premix -- 17..119 of sequence</p>
 +
<p>GACGAGCGCAGCGAGTCAGTGAGCGAGGAAGCCTGCATAACGCGAAGTAATCTTTTCGGT
 +
TTTAAAGAAAAAGGGCAGGGTGGTGACACCTTGCCCTTTTTTG</p>
 +
 
 +
<p>53_premix -- 15..843 of sequence</p>
 +
<p>ATATATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTAAG
 +
GATGATTTCTGGAATTCGCGGCCGCTTCTAGAGAATTTTGTCAAAATAATTTTATTGACA
 +
ACGTCTTATTAACGTTGATATAATTTAAATTTTTTTTGACAAAAATGGGCTCGTGTTGTA
 +
CAATAAATGTCAGCCTGCGGTCCGGTGGCCTTAACTACCAACTCACCGGAGGGTATATAT
 +
GTTTGGACCGGATTTCCCGTTTGCATTTGATGATTGGATCGAACATCCTAAAGGACTTGG
 +
GTCAATTCCGGCAGAAAATCACGGCGCTGAGGTTGCAATTATCGGGGCCGGCATTGCAGG
 +
CTTAGTAGCAGCATATGAATTAATGAAGATGGGTCTGAAACCTGTAGTCTATGAGGCCTC
 +
CAAAATGGGAGGTCGCTTACGGTCACAGGAATTTGAAGGAGCGAAGGGAATTGTTGCGGA
 +
ACTGGGCGGTATGCGCTTTCCAGTCTCCTCCACAGCGTTCTTTCATTACGTTGATAAACT
 +
GGGCCTTGAAAGTCGTCCGTTCCCTAACCCTTTGACGGCGGCCAGTGGCAGCACGGTGAT
 +
TGACCTGGAAGGTACCACGTACTATGCACAGATGTTATCAGATCTCCCAGCCCTGTTCCA
 +
AGAAGTGGCGGATGCATGGGCAGACGCCTTAGAATCAGGCTCTCAATTTGGAGATATTCA
 +
ACAGGCCATTCGTGATTCTGACGTACCGCGTCTCAAGGGAACTGTGGACCAAACTCGTGC
 +
CTTTGTGGGACGATCGCACTTTCTATGACTTTGTAGCGACCTCAAAGGC</p>
 +
 
 +
 
 +
<h2>Sequence results for possible auxin producing colony</h2>
 +
 
 +
<p>55_AUX1 -- 18..841 of sequence</p>
 +
<p>ATTTTCAATAGTATGTTAGAAATCTTTCGTGTTGTGATGACGAATTGTGATGAGCACCAG
 +
CATCTGATTGTTGGTGGCGTACAGCAAGTGCCTGTAGGCCTGTGGTCGCACGTGCCTGAA
 +
CGCTGCGCACATTGGCCAAAGGGTACGTCTCTTTCCTCACTTCATCGGGGTGCCCCGCGC
 +
CCAGGTGTCAAACGTATTGCACGTGCTGAAGATGGTTCATTTACGGTTACAGATAATTGG
 +
GGCGATACACGTCAATACGCTGCCGTTTTGACAACCTGTCAATCGTGGCTGCTTACAACG
 +
CAGATTGAATGTGAGGAATCTTTGTTTTCACAGAAGATGTGGATGGCGCTGGATCGCACG
 +
CGTTACATGCAGTCTTCTAAAACTTTTGTGATGGTTGATCGGCCGTTTTGGAAGGACAAA
 +
GATCCGGAAACGGGTCGCGATCTTATGTCCATGACCCTCACCGATCGCTTAACGCGCGGC
 +
ACATACTTATTTGATAATGGCGACGACAAGCCAGGCGTTATTTGCCTGAGTTACTCATGG
 +
ATGAGTGACGCTTTAAAAATGTTACCGCAACCGATCGAGAAACGCGTCAAATTAGCTTTA
 +
GATGCCCTTAAAAAGATTTACCCGAAAGTGGATATCGCCGCACGTATTATTGGTGACCCG
 +
ATTACTGTGAGTTGGGAAGCGGATCCGCATTTCCTGGGGGCATTTAAAGGCGCGTTACCG
 +
GGCCATTACCGTTACAACCAGCGCATGTACGCGCATTTTATGCAGCAGGACATGCCGTCA
 +
GAACAGCGTGGCATGTTTATTGCCGGTGATGATGTATCTTGACA</p>
 +
 
 +
<p>55_AUX2 -- 16..893 of sequence</p>
 +
<p>CAGTACAACGTCATTGAACGCGGTGTGGGGCATCATGAACCATTTCGGAGGGAAAACCCA
 +
CGCAGAGAATCCGGGTCCTGGCGACGTTTTCCATGAGATTGGACCGATCGCGCTGGGGGA
 +
CTAATAATCGCTGGGACGCCCGCCCCTAGAAAAGGAGGACTAAAAATGCGCGAAATGATT
 +
ACGCTTGAATCTCTGTGCCGCGCATTGGCAGACGAACAGATTGCCGCAGAAGAATTGCGC
 +
GAGCGCGCGCTGGATACAGAGGCTCGCTTAACACTGTTAAATTGCTTCATTCGCGAAGGC
 +
GACGCCGTAAGCCAATTCGGTGAAGCGGATCAGGCGCGTAAAGGAACTTCACTTTGGGGG
 +
GTGCCTGTGAGTTTTAAGGATAACATCTGTGTTCGCGGGCTGCCACTGACGGCGGGTACC
 +
CGCGGCATGAGCGGCTTTATTGCCGATCAGGATGCAGCAATCGTGAGCCAGTTAAAAGCA
 +
TTGGGAGCTGTGGTCGCGGGCAAAAATAACATGCATGAGCTGTCATTTGGCGTCACGAGC
 +
ATCAATCCACATTGGGGCGCGGTTGGAAACCCGGTGGCTCCAGGATATTGCGCCGGAGGT
 +
TCTAGCGGAGGTTCCGCTGCGGCGGTCGCATCTGGAATTGTCCCGCTCAGCGTCGGGACG
 +
GACACAGGCGGCTCAATCCGTATTCCGGCGGCATTTTGCGGCATTACGGGTTTTCGCCCT
 +
ACTACAGGTCGCTTGAGTACGGCGGGGATCATTCCGGTCAGCCACACGAAAGGATTGTGT
 +
CGGCCTCCTGACGCGCACAGCTGGCGACGCTGAATTTGTGTATGGGCTGCTGTCAGGAAA
 +
ACAGCAGAGTTTCCCTCTTAATCGCACGGGACCTTGCG</p>
 +
 
 +
<p>55_AUX3 -- 13..851 of sequence</p>
 +
<p>GATGATGTGTCGGCCTCCTGACGCGCACAGCTGGCGACGCTGAATTTGTGTATGGGCTGC
 +
TGTCAGGAAAACAGCAGAGTTTCCCTCTTAATCGCACGGGACCTTGCCGCATCGGCCTGC
 +
CGGTGAGCATGTGGTCTGACTTAGATGGCGAAGTAGAACGTGCCTGTATCAATGCGCTGA
 +
GCCTCTTACGCAAAACGGGCTTTGAATTTGTTGAGATCGACGATGCGGACATTGTGGAAT
 +
TGAACCAGACATTAACCTTCACGGTGCCGCTGTACGAATTTTTCGCGGATTTTGCTCAAT
 +
CCTTGTTGTCCTTAGGTTGGAAACATGGCATCCATCATATTTTTGCACAAGTGGATGATG
 +
CAAATGTGAAAGGCATTATTAATCACCATCTGGGAGAGGGCGCGATTAAGCCGGCACATT
 +
ATCTGAGTTCTCTCCAGAATGGAGAGCTGCTGAAACGCAAGATGGATGAGCTTTTTGCGC
 +
GCCATCACATTAAACTTTTAGGGTATCCGACGGTCCCGTGTCGCGTGCCTCATCTGGACC
 +
ATGCAGATCGTCCGGAATTTTTTAGCCAGGCCATCCGTAACACAGACCTCGCAAGCAACG
 +
CGATGTTACCAAGCATTACAATTCCGGTCGGTCCGGAAGGCCGCCTGCCGGTCGGCCTTA
 +
GTTTTGATGCGCCTCGTGCTCGTGATGCGTTTCTCTTAAGCAATGTTAGTTTGATCGAAA
 +
AAGTGTTAAAAGGCTAATAATACTAGTAGCGGCCGCTGCAGTCCGGCAAAAAAACGGCAA
 +
GGTGTCACCACCCTGCCCTTTTTCTTTAAAACCGAAAAGATTACTTCGCGTTATGCAGC</p>
 +
 
 +
<p>55_premix -- 16..869 of sequence</p>
 +
<p>ACGAGCGCAGCGAGTCAGTGAGCGAGGAAGCCTGCATAACGCGAAGTAATCTTTTCGGTT
 +
TTAAAGAAAAAGGGCAGGGTGGTGACACCTTGCCGTTTTTTTGCCGGACTGCAGCGGCCG
 +
CTACTAGTATTATTAGCCTTTTAACACTTTTTCGATCAAACTAACATTGCTTAAGAGAAA
 +
CGCATCACGAGCACGAGGCGCATCAAAACTAAGGCCGACCGGCAGGCGGCCTTCCGGACC
 +
GACCGGAATTGTAATGCTTGGTAACATCGCGTTGCTTGCGAGGTCTGTGTTACGGATGGC
 +
CTGGCTAAAAAATTCCGGACGATCTGCATGGTCCAGATGAGGCACGCGACACGGGACCGT
 +
CGGATACCCTAAAAGTTTAATGTGATGGCGCGCAAAAAGCTCATCCATCTTGCGTTTCAG
 +
CAGCTCTCCATTCTGGAGAGAACTCAGATAATGTGCCGGCTTAATCGCGCCCTCTCCCAG
 +
ATGGTGATTAATAATGCCTTTCACATTTGCATCATCCACTTGTGCAAAAATATGATGGAT
 +
GCCATGTTTCCAACCTAAGGACAACAAGGATTGAGCAAAATCCGCGAAAAATTCGTACAG
 +
CGGCACCGTGAAGGTTAATGTCTGGTTCAATTCCACAATGTCCGCATCGTCGATCTCAAC
 +
AAATTCAAAGCCCGTTTTGCGTAAGAGGCTCAGCGCATTGATACAGGCACGTTCTACTTC
 +
GCCATCTAAGTCAGACCACATGCTCACCGGCAGGCCGATGCGGCAAGGTCCCGTGCGATT
 +
AAGAGGGAAACTCTGCTGTTTTCCTGACAGCAGCCCATACACAAATTCAGCGTCGCCAGC
 +
TGTGCGCGTCAGAG</p>
 +
 
 +
<p>55_premix -- 14..888 of sequence</p>
 +
<p>CTATAAAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCT
 +
AAGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGAATTTTGTCAAAATAATTTTATTG
 +
ACAACGTCTTATTAACGTTGATATAATTTAAATTTTTTTTGACAAAAATGGGCTCGTGTT
 +
GTACAATAAATGTCAGCCTGCGGTCCGGTGGCCTTAACTACCAACTCACCGGAGGGTATA
 +
TATGTTTGGACCGGATTTCCCGTTTGCATTTGATGATTGGATCGAACATCCTAAAGGACT
 +
TGGGTCAATTCCGGCAGAAAATCACGGCGCTGAGGTTGCAATTATCGGGGCCGGCATTGC
 +
AGGCTTAGTAGCAGCATATGAATTAATGAAGATGGGTCTGAAACCTGTAGTCTATGAGGC
 +
CTCCAAAATGGGAGGTCGCTTACGGTCACAGGAATTTGAAGGAGCGAAGGGAATTGTTGC
 +
GGAACTGGGCGGTATGCGCTTTCCAGTCTCCTCCACAGCGTTCTTTCATTACGTTGATAA
 +
ACTGGGCCTTGAAAGTCGTCCGTTCCCTAACCCTTTGACGGCGGCCAGTGGCAGCACGGT
 +
GATTGACCTGGAAGGTACCACGTACTATGCACAGATGTTATCAGATCTCCCAGCCCTGTT
 +
CCAAGAAGTGGCGGATGCATGGGCAGACGCCTTAGAATCAGGCTCTCAATTTGGAGATAT
 +
TCAACAGGCCATTCGTGATTCTGACGTACCGCGTCTCAAGGAACTGTGGAACAAACTCGT
 +
GCCTTTGTGGGACGATCGCACTTTCTATGACTTTGTAGCGACCTCAAAGGCCTTTGCGAA
 +
ATTAAGCTTTTACCATCGTGAGGTTTCGGACAGTT</p>
 +
 
 +
<h2>Sequence results for our Auxin Xpress module.</h2>
 +
 
 +
<p>56_AUX1 -- 21..841 of sequence</p>
 +
<p>TTCAATAGTATGTTAGAAATCTTTCGTGTTGTGATGACGAATTGTGATGAGCACCAGCAT
 +
CTGATTGTTGGTGGCGTACAGCAAGTGCCTGTAGGCCTGTGGTCGCACGTGCCTGAACGC
 +
TGCGCACATTGGCCAAAGGGTACGTCTCTTTCCTCACTTCATCGGGGTGCCCCGCGCCCA
 +
GGTGTCAAACGTATTGCACGTGCTGAAGATGGTTCATTTACGGTTACAGATAATTGGGGC
 +
GATACACGTCAATACGCTGCCGTTTTGACAACCTGTCAATCGTGGCTGCTTACAACGCAG
 +
ATTGAATGTGAGGAATCTTTGTTTTCACAGAAGATGTGGATGGCGCTGGATCGCACGCGT
 +
TACATGCAGTCTTCTAAAACTTTTGTGATGGTTGATCGGCCGTTTTGGAAGGACAAAGAT
 +
CCGGAAACGGGTCGCGATCTTATGTCCATGACCCTCACCGATCGCTTAACGCGCGGCACA
 +
TACTTATTTGATAATGGCGACGACAAGCCAGGCGTTATTTGCCTGAGTTACTCATGGATG
 +
AGTGACGCTTTAAAAATGTTACCGCAACCGATCGAGAAACGCGTCAAATTAGCTTTAGAT
 +
GCCCTTAAAAAGATTTACCCGAAAGTGGATATCGCCGCACGTATTATTGGTGACCCGATT
 +
ACTGTGAGTTGGGAAGCGGATCCGCATTTCCTGGGGGCATTTAAAGGCGCGTTACCGGGC
 +
CATTACCGTTACAACCAGCGCATGTACGCGCATTTTATGCAGCAGGACATGCCGTCAGAA
 +
CAGCGTGGCATGTTTATTGCCGGTGATGATGTATCTTGACA</p>
 +
 
 +
<p>56_AUX2 -- 16..912 of sequence</p>
 +
<p>GCAGTACAACGTCATTGAACGCGGTGTGGGGCATCATGAACCATTTCGGAGGGAAAACCC
 +
ACGCAGAGAATCCGGGTCCTGGCGACGTTTTCCATGAGATTGGACCGATCGCGCTGGGGG
 +
ACTAATAATCGCTGGGACGCCCGCCCCTAGAAAAGGAGGACTAAAAATGCGCGAAATGAT
 +
TACGCTTGAATCTCTGTGCCGCGCATTGGCAGACGAACAGATTGCCGCAGAAGAATTGCG
 +
CGAGCGCGCGCTGGATACAGAGGCTCGCTTAACACTGTTAAATTGCTTCATTCGCGAAGG
 +
CGACGCCGTAAGCCAATTCGGTGAAGCGGATCAGGCGCGTAAAGGAACTTCACTTTGGGG
 +
GGTGCCTGTGAGTTTTAAGGATAACATCTGTGTTCGCGGGCTGCCACTGACGGCGGGTAC
 +
CCGCGGCATGAGCGGCTTTATTGCCGATCAGGATGCAGCAATCGTGAGCCAGTTAAAAGC
 +
ATTGGGAGCTGTGGTCGCGGGCAAAAATAACATGCATGAGCTGTCATTTGGCGTCACGAG
 +
CATCAATCCACATTGGGGCGCGGTTGGAAACCCGGTGGCTCCAGGATATTGCGCCGGAGG
 +
TTCTAGCGGAGGTTCCGCTGCGGCGGTCGCATCTGGAATTGTCCCGCTCAGCGTCGGGAC
 +
GGACACAGGCGGCTCAATCCGTATTCCGGCGGCATTTTGCGGCATTACGGGTTTTCGCCC
 +
TACTACAGGTCGCTTGAGTACGGCGGGGATCATTCCGGTCAGCCACACGAAGGATTGTGT
 +
CGGCCTCCTGACGCGCACAGCTGGCGACGCTGAATTTGTGTATGGGCTGCTGTCAGGAAA
 +
ACAGCAGAGTTTCCCTCTTAATCGCACGGGACCTTGCCGCATCGGCCTGCCGGTGAG</p>
 +
 
 +
<p>56_AUX3 -- 15..922 of sequence</p>
 +
<p>GATGATGTGTCGGCCTCCTGACGCGCACAGCTGGCGACGCTGAATTTGTGTATGGGCTGC
 +
TGTCAGGAAAACAGCAGAGTTTCCCTCTTAATCGCACGGGACCTTGCCGCATCGGCCTGC
 +
CGGTGAGCATGTGGTCTGACTTAGATGGCGAAGTAGAACGTGCCTGTATCAATGCGCTGA
 +
GCCTCTTACGCAAAACGGGCTTTGAATTTGTTGAGATCGACGATGCGGACATTGTGGAAT
 +
TGAACCAGACATTAACCTTCACGGTGCCGCTGTACGAATTTTTCGCGGATTTTGCTCAAT
 +
CCTTGTTGTCCTTAGGTTGGAAACATGGCATCCATCATATTTTTGCACAAGTGGATGATG
 +
CAAATGTGAAAGGCATTATTAATCACCATCTGGGAGAGGGCGCGATTAAGCCGGCACATT
 +
ATCTGAGTTCTCTCCAGAATGGAGAGCTGCTGAAACGCAAGATGGATGAGCTTTTTGCGC
 +
GCCATCACATTAAACTTTTAGGGTATCCGACGGTCCCGTGTCGCGTGCCTCATCTGGACC
 +
ATGCAGATCGTCCGGAATTTTTTAGCCAGGCCATCCGTAACACAGACCTCGCAAGCAACG
 +
CGATGTTACCAAGCATTACAATTCCGGTCGGTCCGGAAGGCCGCCTGCCGGTCGGCCTTA
 +
GTTTTGATGCGCCTCGTGCTCGTGATGCGTTTCTCTTAAGCAATGTTAGTTTGATCGAAA
 +
AAGTGTTAAAAGGCTAATAATACTAGTAGCGGCCGCTGCAGTCCGGCAAAAAAACGGCAA
 +
GGTGTCACCACCCTGCCCTTTTTCTTTAAAACCGAAAAGATTACTTCGCGTTATGCACGC
 +
TTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCA
 +
CTCAACGC</p>
 +
 
 +
<p>56_premix -- 14..930 of sequence</p>
 +
<p>TATAAAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTA
 +
AGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGAATTTTGTCAAAATAATTTTATTGA
 +
CAACGTCTTATTAACGTTGATATAATTTAAATTTTTTTTGACAAAAATGGGCTCGTGTTG
 +
TACAATAAATGTCAGCCTGCGGTCCGGTGGCCTTAACTACCAACTCACCGGAGGGTATAT
 +
ATGTTTGGACCGGATTTCCCGTTTGCATTTGATGATTGGATCGAACATCCTAAAGGACTT
 +
GGGTCAATTCCGGCAGAAAATCACGGCGCTGAGGTTGCAATTATCGGGGCCGGCATTGCA
 +
GGCTTAGTAGCAGCATATGAATTAATGAAGATGGGTCTGAAACCTGTAGTCTATGAGGCC
 +
TCCAAAATGGGAGGTCGCTTACGGTCACAGGAATTTGAAGGAGCGAAGGGAATTGTTGCG
 +
GAACTGGGCGGTATGCGCTTTCCAGTCTCCTCCACAGCGTTCTTTCATTACGTTGATAAA
 +
CTGGGCCTTGAAAGTCGTCCGTTCCCTAACCCTTTGACGGCGGCCAGTGGCAGCACGGTG
 +
ATTGACCTGGAAGGTACCACGTACTATGCACAGATGTTATCAGATCTCCCAGCCCTGTTC
 +
CAAGAAGTGGCGGATGCATGGGCAGACGCCTTAGAATCAGGCTCTCAATTTGGAGATATT
 +
CAACAGGCCATTCGTGATTCTGACGTACCGCGTCTCAAGGAACTGTGGAACAAACTCGTG
 +
CCTTTGTGGGACGATCGCACTTTCTATGACTTTGTAGCGACCTCAAAGGCCTTTGCGAAA
 +
TTAAGCTTTTACCATCGTGAGGTTTTCGGACAAGTTGGTTTTGGTACAGGGGGTGGGATT
 +
CTGATTTTCCAATAGTA</p>
 +
 
 +
<p>56_premix -- 15..893 of sequence</p>
 +
<p>GACGAGCGCAGCGAGTCAGTGAGCGAGGAAGCCTGCATAACGCGAAGTAATCTTTTCGGT
 +
TTTAAAGAAAAAGGGCAGGGTGGTGACACCTTGCCGTTTTTTTGCCGGACTGCAGCGGCC
 +
GCTACTAGTATTATTAGCCTTTTAACACTTTTTCGATCAAACTAACATTGCTTAAGAGAA
 +
ACGCATCACGAGCACGAGGCGCATCAAAACTAAGGCCGACCGGCAGGCGGCCTTCCGGAC
 +
CGACCGGAATTGTAATGCTTGGTAACATCGCGTTGCTTGCGAGGTCTGTGTTACGGATGG
 +
CCTGGCTAAAAAATTCCGGACGATCTGCATGGTCCAGATGAGGCACGCGACACGGGACCG
 +
TCGGATACCCTAAAAGTTTAATGTGATGGCGCGCAAAAAGCTCATCCATCTTGCGTTTCA
 +
GCAGCTCTCCATTCTGGAGAGAACTCAGATAATGTGCCGGCTTAATCGCGCCCTCTCCCA
 +
GATGGTGATTAATAATGCCTTTCACATTTGCATCATCCACTTGTGCAAAAATATGATGGA
 +
TGCCATGTTTCCAACCTAAGGACAACAAGGATTGAGCAAAATCCGCGAAAAATTCGTACA
 +
GCGGCACCGTGAAGGTTAATGTCTGGTTCAATTCCACAATGTCCGCATCGTCGATCTCAA
 +
CAAATTCAAAGCCCGTTTTGCGTAAGAGGCTCAGCGCATTGATACAGGCACGTTCTACTT
 +
CGCCATCTAAGTCAGACCACATGCTCACCGGCAGGCCGATGCGGCAAGGTCCCGTGCGAT
 +
TAAGAGGGAAACTCTGCTGTTTTCCTGACAGCAGCCCATACACAAATTCAGCGTCGCCAG
 +
CTGTGCGCGTCAGGAGGCCGACACAATCCTTCGTGTGGC</p>
 +
 
 +
<h2>Sequence results to determine SpeI site problem in sfGFP</h2>
 +
 
 +
<p>17R_premix -- 35..933 of sequence</p>
 +
<p>AGCGAGTCGGTGAGCGAGGAAGCCTGCATAACGCGAAGTAATCTTTTCGGTTTTAAAGAA
 +
AAAGGGCAGGGTGGTGACACCTTGCCCTTTTTTGCCGGACTGCAGCGGCCGCTACTAGGT
 +
AGCGAAAAAACCCCGCCGAAGCGGGGTTTTTTGCGCTCTAGTATCATTACTTATACAGCT
 +
CGTCCATACCGTGGGTGATGCCCGCAGCGGTCACAAATTCCAACAGGACCATGTGATCAC
 +
GTTTTTCGTTCGGATCTTTGCTCAGCACGCTTTGGGTGCTCAGATAGTGATTATCCGGCA
 +
GCAAAACCGGACCGTCACCAATTGGGGTGTTCTGCTGGTAATGGTCGGCCAGTTGGACGC
 +
TACCGTCCTCAACATTGTGGCGAATCTTGAAATTGGCCTTGATGCCGTTCTTTTGCTTGT
 +
CTGCCGTAATGTAAACATTATGGCTGTTGAAGTTATACTCCAGTTTATGGCCCAGAATGT
 +
TACCATCCTCTTTAAAGTCGATACCCTTCAATTCGATACGATTGACCAGCGTGTCACCTT
 +
CAAACTTAACCTCTGCGCGAGTTTTGTAGTAGCCATCATCCTTGAAGGAAATGGTACGCT
 +
CCTGGACGTAACCCTCCGGCATCGCAGATTTGAAGAAATCGTGTTGTTTCATGTGGTCCG
 +
GATAACGCGCAAAACACTGCACGCCATACGTCAACGTCGTCACCAAGGTCGGCCAAGGCA
 +
CCGGCAGTTTACCGGTGGTGCAGATGAACTTCAGGGTCAGTTTGCCGTTGGTCGCGTCGC
 +
CCTCACCTTCACCGCGAACGCTAAACTTATGACCGTTCACATCGCCGTCCAGCTCGACCA
 +
GAATCGGAACTACGCCCGTGAACAGTTCTTCGCCTTTACGCATTATCTTACCTCCTTAA</p>
 +
 
 +
<h2>Sequence results for possible PA2652 producing colony</h2>
 +
 
 +
<p>57.20F_premix -- 14..986 of sequence</p>
 +
<p>CTATAAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTA
 +
AGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGTTGACGGCTAGCTCAGTCCTAGGTA
 +
CAGTGCTAGCCAGCCTGCGGTCCGGTTAAAAAGAAGGGGTTCTAAATGATGCGCTTGACC
 +
CTGAAAAGTAAAGTTCTGCTGCTGGCCATGGTACCGGTGCTTTTGTTTGCACTGGTACTG
 +
TCAGGCGGCGCCGTACTGATTCTGAAAAAGCAAGCCGATGCCGAAGTCAAAGATACCCGC
 +
GAGCGCCTTTTAGGGGATCGCCGCGCGGAACTGGAGCACTACGTGCAAATCGCCATGGGC
 +
AGTATTCAAGCAGAATATGATCGCAGCGCGAACGGTGACCTCAACGCCCGCGCCGAAGCC
 +
ATTGCGCGCCTGAGCAAGATTAAATATGGAAAAGATGGTTACATTTTCGGATACGATTCT
 +
CAGGTCGTGCGGCTGTTTCGCGGGGATTCTCCTGTAGATGTAGGCAAAAGTTTTCGCGAT
 +
CGTCGTGATCCGAGCGGAGTGTACTTGAATCGTGAACTTGTCGAAGCCGGACGCAACGGT
 +
TCCCACTACGTAACCTATACCTCGCCGCTGCCGGGTAACGAATCGGTGATGGTTCCTAAA
 +
CTGTCGTACACGCTGTATCTGCCGAAGTGGGATATGGTAATCGGTAGCGCGATTAATTTG
 +
GACGGTGTCGAAGCGCAACTGGTAGAAATTAAACAGGATATTGATGAACGCATCGGCACC
 +
CTGATTGCGAGCATTGTGGGTATTGCCGGCGTACTGCTGGTAGTGCTGCTGGTGATTGGG
 +
TTAGCAGTGGCGAATGCTATGCTTCGCCCGCTTCATCAGATCCGTCAGAACCTTGATGAT
 +
ATTGCCGCCGGCGAGGGTGATCTGACCCGCCGCCTGCCGGTGACCAGCTACGATGAACTC
 +
GGTGAACTGGCAG</p>
 +
 
 +
<p>57.20R_premix -- 13..1023 of sequence</p>
 +
<p>ACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCCTGCATAACGCGAAGTAATCTTTTC
 +
GGTTTTAAAGAAAAAGGGCAGGGTGGTGACACCTTGCCCTTTTTTGCCGGACTGCAGCGG
 +
CCGCTACTAGTATTATTAGATGCGAAACTGGCCCACCAGACGACCTAAGCCTTGACCCAG
 +
CTGCGCCAGTGACCGAGCCGTTTGTGCACCTTGCTGTGCTTCATCCGCCACTGAATCAAC
 +
CGCGCCCGCGATCTGGTGTACAGAGCGATTAATCTCTTCTGCCACTGCGGTTTGTTCTTC
 +
TGCCGCGCTCGCGATCTGCGCATTCATCGCATTAATGGTGGCAATCAGCTGGGCGATCGC
 +
ATCCAGGGAATCGCCTGCCTGATTCGCCTGATTTGAGGTTCCCTCTCCCGCTTCACCGCT
 +
GCGCCGCATGGCATCAACGGCGGCATTGGTGCCCTGTTGCAGACGATCGATCATACCCTG
 +
GATTTCCTGGGTGCTCTGCTGGGTGCGAGAAGCCAGTGCGCGCACCTCATCCGCGACGAC
 +
GGCAAATCCACGACCTGCTTCGCCTGCACGTGCTGCCTCAATGGCCGCATTCAGGGCCAG
 +
CAAATTAGTTTGTTCCGCAATGGAACGAATCACGCCCAGCACCGAGACAATGCTCTGGAC
 +
ATCTTGCTGAAGGCTATCGAGACTGGTGCCCGAATCGCGGATTTCGTCTACCAGGGCATG
 +
AATACGCTCAATAGAACCATCCACTACACGCTTAGCTGCCTGGCCTTCATGATCTGTTTG
 +
CTGAGCTGCCTCGGCTGCGCGTTGAGCGCTTTGTGCCACTTCGTGTGCGGCGGCGCTCAT
 +
CTCGTTAATCGCGGTGGCAACCTGATCGGTCTCGTGACGCTGACGTTCCATCGCTTGTTC
 +
AGAACGTTCTGCCTGCGCGCTCATTTGCTCCACTAACTGTTTCAGATCGCCCGTCATCCC
 +
TGCGATCTGGCGAACTAAGCCATGAATTTTTCAACGAAACGGTTAAAGGAG</p>
 +
 
 +
<h2>Sequence results for possible PA2652 producing colony</h2>
 +
 
 +
<p>57.3F_premix -- 15..985 of sequence</p>
 +
<p>TATAAAAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCT
 +
AAGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGTTGACGGCTAGCTCAGTCCTAGGT
 +
ACAGTGCTAGCCAGCCTGCGGTCCGGTTAAAAAGAAGGGGTTCTAAATGATGCGCTTGAC
 +
CCTGAAAAGTAAAGTTCTGCTGCTGGCCATGGTACCGGTGCTTTTGTTTGCACTGGTACT
 +
GTCAGGCGGCGCCGTACTGATTCTGAAAAAGCAAGCCGATGCCGAAGTCAAAGATACCCG
 +
CGAGCGCCTTTTAGGGGATCGCCGCGCGGAACTGGAGCACTACGTGCAAATCGCCATGGG
 +
CAGTATTCAAGCAGAATATGATCGCAGCGCGAACGGTGACCTCAACGCCCGCGCCGAAGC
 +
CATTGCGCGCCTGAGCAAGATTAAATATGGAAAAGATGGTTACATTTTCGGATACGATTC
 +
TCAGGTCGTGCGGCTGTTTCGCGGGGATTCTCCTGTAGATGTAGGCAAAAGTTTTCGCGA
 +
TCGTCGTGATCCGAGCGGAGTGTACTTGAATCGTGAACTTGTCGAAGCCGGACGCAACGG
 +
TTCCCACTACGTAACCTATACCTCGCCGCTGCCGGGTAACGAATCGGTGATGGTTCCTAA
 +
ACTGTCGTACACGCTGTATCTGCCGAAGTGGGATATGGTAATCGGTAGCGCGATTAATTT
 +
GGACGGTGTCGAAGCGCAACTGGTAGAAATTAAACAGGATATTGATGAACGCATCGGCAC
 +
CCTGATTGCGAGCATTGTGGGTATTGCCGGCGTACTGCTGGTAGTGCTGCTGGTGATTGG
 +
GTTAGCAGTGGCGAATGCTATGCTTCGCCCGCTTCATCAGATCCGTCAGAACCCTTGATG
 +
ATATTGCCGCCGGCGAGGGTGATCTGACCCGCCGCCTGCCGGTGACCAGCTACGATGAAC
 +
TCGGTGAACTG</p>
 +
 
 +
<p>57.3R_premix -- 15..1019 of sequence</p>
 +
<p>ACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCCTGCATAACGCGAAGTAATCTTTTC
 +
GGTTTTAAAGAAAAAGGGCAGGGTGGTGACACCTTGCCCTTTTTTGCCGGACTGCAGCGG
 +
CCGCTACTAGTATTATTAGATGCGAAACTGGCCCACCAGACGACCTAAGCCTTGACCCAG
 +
CTGCGCCAGTGACCGAGCCGTTTGTGCACCTTGCTGTGCTTCATCCGCCACTGAATCAAC
 +
CGCGCCCGCGATCTGGTGTACAGAGCGATTAATCTCTTCTGCCACTGCGGTTTGTTCTTC
 +
TGCCGCGCTCGCGATCTGCGCATTCATCGCATTAATGGTGGCAATCAGCTGGGCGATCGC
 +
ATCCAGGGAATCGCCTGCCTGATTCGCCTGATTTGAGGTTCCCTCTCCCGCTTCACCGCT
 +
GCGCCGCATGGCATCAACGGCGGCATTGGTGCCCTGTTGCAGACGATCGATCATACCCTG
 +
GATTTCCTGGGTGCTCTGCTGGGTGCGAGAAGCCAGTGCGCGCACCTCATCCGCGACGAC
 +
GGCAAATCCACGACCTGCTTCGCCTGCACGTGCTGCCTCAATGGCCGCATTCAGGGCCAG
 +
CAAATTAGTTTGTTCCGCAATGGAACGAATCACGCCCAGCACCGAGACAATGCTCTGGAC
 +
ATCTTGCTGAAGGCTATCGAGACTGGTGCCCGAATCGCGGATTTCGTCTACCAGGGCATG
 +
AATACGCTCAATAGAACCATCCACTACACGCTTAGCTGCCTGGCCTTCATGATCTGTTTG
 +
CTGAGCTGCCTCGGCTGCGCGTTGAGCGCTTTGTGCCACTTCGTGTGCGGCGGCGCTCAT
 +
CTCGTTAATCGCGGTGGCAACCTGATCGGTCTCGTGACGCTGACGTTCCATCGCTTGTTC
 +
AGAACGTTCTGCCTGCGCGCTCATTTGCTCCACTAACTGTTTCAGATCGCCCGTCATCCC
 +
TGCGATCTGGCGAACTAAGCCATGAATTTTTTCAACGAAACGGTT</p>
 +
 
 +
<h2>Sequence results for possible PA2652 producing colony</h2>
 +
 
 +
<p>57.7F_premix -- 14..987 of sequence</p>
 +
<p>TATAAAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTA
 +
AGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGTTGACGGCTAGCTCAGTCCTAGGTA
 +
CAGTGCTAGCCAGCCTGCGGTCCGGTTAAAAAGAAGGGGTTCTAAATGATGCGCTTGACC
 +
CTGAAAAGTAAAGTTCTGCTGCTGGCCATGGTACCGGTGCTTTTGTTTGCACTGGTACTG
 +
TCAGGCGGCGCCGTACTGATTCTGAAAAAGCAAGCCGATGCCGAAGTCAAAGATACCCGC
 +
GAGCGCCTTTTAGGGGATCGCCGCGCGGAACTGGAGCACTACGTGCAAATCGCCATGGGC
 +
AGTATTCAAGCAGAATATGATCGCAGCGCGAACGGTGACCTCAACGCCCGCGCCGAAGCC
 +
ATTGCGCGCCTGAGCAAGATTAAATATGGAAAAGATGGTTACATTTTCGGATACGATTCT
 +
CAGGTCGTGCGGCTGTTTCGCGGGGATTCTCCTGTAGATGTAGGCAAAAGTTTTCGCGAT
 +
CGTCGTGATCCGAGCGGAGTGTACTTGAATCGTGAACTTGTCGAAGCCGGACGCAACGGT
 +
TCCCACTACGTAACCTATACCTCGCCGCTGCCGGGTAACGAATCGGTGATGGTTCCTAAA
 +
CTGTCGTACACGCTGTATCTGCCGAAGTGGGATATGGTAATCGGTAGCGCGATTAATTTG
 +
GACGGTGTCGAAGCGCAACTGGTAGAAATTAAACAGGATATTGATGAACGCATCGGCACC
 +
CTGATTGCGAGCATTGTGGGTATTGCCGGCGTACTGCTGGTAGTGCTGCTGGTGATTGGG
 +
TTAGCAGTGGCGAATGCTATGCTTCGCCCGCTTCATCAGATCCGTCAGAACCTTGATGAT
 +
ATTGCCGCCGGCGAGGGTGATCTGACCCGCCGCCTGCCGGTGACCAGCTACGATGAACTC
 +
GGTGAACTGGCAGC</p>
 +
 
 +
<p>57.7R_premix -- 15..1032 of sequence</p>
 +
<p>GACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCCTGCATAACGCGAAGTAATCTTTT
 +
CGGTTTTAAAGAAAAAGGGCAGGGTGGTGACACCTTGCCCTTTTTTGCCGGACTGCAGCG
 +
GCCGCTACTAGTATTATTAGATGCGAAACTGGCCCACCAGACGACCTAAGCCTTGACCCA
 +
GCTGCGCCAGTGACCGAGCCGTTTGTGCACCTTGCTGTGCTTCATCCGCCACTGAATCAA
 +
CCGCGCCCGCGATCTGGTGTACAGAGCGATTAATCTCTTCTGCCACTGCGGTTTGTTCTT
 +
CTGCCGCGCTCGCGATCTGCGCATTCATCGCATTAATGGTGGCAATCAGCTGGGCGATCG
 +
CATCCAGGGAATCGCCTGCCTGATTCGCCTGATTTGAGGTTCCCTCTCCCGCTTCACCGC
 +
TGCGCCGCATGGCATCAACGGCGGCATTGGTGCCCTGTTGCAGACGATCGATCATACCCT
 +
GGATTTCCTGGGTGCTCTGCTGGGTGCGAGAAGCCAGTGCGCGCACCTCATCCGCGACGA
 +
CGGCAAATCCACGACCTGCTTCGCCTGCACGTGCTGCCTCAATGGCCGCATTCAGGGCCA
 +
GCAAATTAGTTTGTTCCGCAATGGAACGAATCACGCCCAGCACCGAGACAATGCTCTGGA
 +
CATCTTGCTGAAGGCTATCGAGACTGGTGCCCGAATCGCGGATTTCGTCTACCAGGGCAT
 +
GAATACGCTCAATAGAACCATCCACTACACGCTTAGCTGCCTGGCCTTCATGATCTGTTT
 +
GCTGAGCTGCCTCGGCTGCGCGTTGAGCGCTTTGTGCCACTTCGTGTGCGGCGGCGCTCA
 +
TCTCGTTAATCGCGGTGGCAACCTGATCGGTCTCGTGACGCTGACGTTCCATCGCTTGTT
 +
CAGAACGTTCTGCCTGCGCGCTCATTTGCTCCACTAACTGTTTCAGATCGCCCGTCATCC
 +
CTGCGATCTGGCGAACTAAGCCATGAATTTTTTCAACGAAACGGTTAAAGGAGCCTGC</p>
 +
 
 +
<h2>Sequence results for our PA2652 producing construct. Correct.</h2>
 +
 
 +
<p>57.8F_premix -- 15..979 of sequence</p>
 +
<p>TATAAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTAA
 +
GGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGTTGACGGCTAGCTCAGTCCTAGGTAC
 +
AGTGCTAGCCAGCCTGCGGTCCGGTTAAAAAGAAGGGGTTCTAAATGATGCGCTTGACCC
 +
TGAAAAGTAAAGTTCTGCTGCTGGCCATGGTACCGGTGCTTTTGTTTGCACTGGTACTGT
 +
CAGGCGGCGCCGTACTGATTCTGAAAAAGCAAGCCGATGCCGAAGTCAAAGATACCCGCG
 +
AGCGCCTTTTAGGGGATCGCCGCGCGGAACTGGAGCACTACGTGCAAATCGCCATGGGCA
 +
GTATTCAAGCAGAATATGATCGCAGCGCGAACGGTGACCTCAACGCCCGCGCCGAAGCCA
 +
TTGCGCGCCTGAGCAAGATTAAATATGGAAAAGATGGTTACATTTTCGGATACGATTCTC
 +
AGGTCGTGCGGCTGTTTCGCGGGGATTCTCCTGTAGATGTAGGCAAAAGTTTTCGCGATC
 +
GTCGTGATCCGAGCGGAGTGTACTTGAATCGTGAACTTGTCGAAGCCGGACGCAACGGTT
 +
CCCACTACGTAACCTATACCTCGCCGCTGCCGGGTAACGAATCGGTGATGGTTCCTAAAC
 +
TGTCGTACACGCTGTATCTGCCGAAGTGGGATATGGTAATCGGTAGCGCGATTAATTTGG
 +
ACGGTGTCGAAGCGCAACTGGTAGAAATTAAACAGGATATTGATGAACGCATCGGCACCC
 +
TGATTGCGAGCATTGTGGGTATTGCCGGCGTACTGCTGGTAGTGCTGCTGGTGATTGGGT
 +
TAGCAGTGGCGAATGCTATGCTTCGCCCGCTTCATCAGATCCGTCAGAACCCTTGATGAT
 +
ATTGCCGCCGGCGAGGGTGATCTGACCCGCCGCCTGCCGGTGACCAGCTACGATGACTCG
 +
GTGAC</p>
 +
 
 +
<p>57.8R_premix -- 14..1022 of sequence</p>
 +
<p>ACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCCTGCATAACGCGAAGTAATCTTTTC
 +
GGTTTTAAAGAAAAAGGGCAGGGTGGTGACACCTTGCCCTTTTTTGCCGGACTGCAGCGG
 +
CCGCTACTAGTATTATTAGATGCGAAACTGGCCCACCAGACGACCTAAGCCTTGACCCAG
 +
CTGCGCCAGTGACCGAGCCGTTTGTGCACCTTGCTGTGCTTCATCCGCCACTGAATCAAC
 +
CGCGCCCGCGATCTGGTGTACAGAGCGATTAATCTCTTCTGCCACTGCGGTTTGTTCTTC
 +
TGCCGCGCTCGCGATCTGCGCATTCATCGCATTAATGGTGGCAATCAGCTGGGCGATCGC
 +
ATCCAGGGAATCGCCTGCCTGATTCGCCTGATTTGAGGTTCCCTCTCCCGCTTCACCGCT
 +
GCGCCGCATGGCATCAACGGCGGCATTGGTGCCCTGTTGCAGACGATCGATCATACCCTG
 +
GATTTCCTGGGTGCTCTGCTGGGTGCGAGAAGCCAGTGCGCGCACCTCATCCGCGACGAC
 +
GGCAAATCCACGACCTGCTTCGCCTGCACGTGCTGCCTCAATGGCCGCATTCAGGGCCAG
 +
CAAATTAGTTTGTTCCGCAATGGAACGAATCACGCCCAGCACCGAGACAATGCTCTGGAC
 +
ATCTTGCTGAAGGCTATCGAGACTGGTGCCCGAATCGCGGATTTCGTCTACCAGGGCATG
 +
AATACGCTCAATAGAACCATCCACTACACGCTTAGCTGCCTGGCCTTCATGATCTGTTTG
 +
CTGAGCTGCCTCGGCTGCGCGTTGAGCGCTTTGTGCCACTTCGTGTGCGGCGGCGCTCAT
 +
CTCGTTAATCGCGGTGGCAACCTGATCGGTCTCGTGACGCTGACGTTCCATCGCTTGTTC
 +
AGAACGTTCTGCCTGCGCGCTCATTTGCTCCACTAACTGTTTCAGATCGCCCGTCATCCC
 +
TGCGATCTGGCGAACTAAGCCATGAATTTTTCAACGAAACGGTTAAAGG</p>
 +
 
 +
<h2>Dendra2 forward sequencing results after ligation with Ptet and RBS</h2>
 +
 
 +
<p> Dendra_forward_premix -- 15..179 of sequence</p>
 +
<p>TATAAAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTA
 +
AGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGTCCCTATCAGTGATAGAGATTGACA
 +
TCCCTATCAGTGATAGAGATACTGAGCACTACTAGATGAACATCA</p>
 +
 
 +
<h2>Results of J23103 ligation into RFP. Correct but Kirsten says suffix is strange</h2>
 +
 
 +
<p> 21+60a_frwrd_premix -- 15..921 of sequence</p>
 +
<p>ATAATAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTA
 +
AGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGCTGATAGCTAGCTCAGTCCTAGGGA
 +
TTATGCTAGCTACTAGAGAAAGAGGAGAAATACTAGATGGCTTCCTCCGAAGACGTTATC
 +
AAAGAGTTCATGCGTTTCAAAGTTCGTATGGAAGGTTCCGTTAACGGTCACGAGTTCGAA
 +
ATCGAAGGTGAAGGTGAAGGTCGTCCGTACGAAGGTACCCAGACCGCTAAACTGAAAGTT
 +
ACCAAAGGTGGTCCGCTGCCGTTCGCTTGGGACATCCTGTCCCCGCAGTTCCAGTACGGT
 +
TCCAAAGCTTACGTTAAACACCCGGCTGACATCCCGGACTACCTGAAACTGTCCTTCCCG
 +
GAAGGTTTCAAATGGGAACGTGTTATGAACTTCGAAGACGGTGGTGTTGTTACCGTTACC
 +
CAGGACTCCTCCCTGCAAGACGGTGAGTTCATCTACAAAGTTAAACTGCGTGGTACCAAC
 +
TTCCCGTCCGACGGTCCGGTTATGCAGAAAAAAACCATGGGTTGGGAAGCTTCCACCGAA
 +
CGTATGTACCCGGAAGACGGTGCTCTGAAAGGTGAAATCAAAATGCGTCTGAAACTGAAA
 +
GACGGTGGTCACTACGACGCTGAAGTTAAAACCACCTACATGGCTAAAAAACCGGTTCAG
 +
CTGCCGGGTGCTTACAAAACCGACATCAAACTGGACATCACCTCCCACAACGAAGACTAC
 +
ACCATCGTTGAACAGTACGAACGTGCTGAAGGTCGTCACTCCACCGGTGCTTAATAACGC
 +
TGATAGTGCTAGTGTAGATCGCTACTAGAGATTAAAGAGGAGAAATACTAGTAGCGGCCG
 +
CTGCAGG</p>
 +
 
 +
<h2>Results of J23103 ligation into RFP. Correct but Kirsten says suffix is strange</h2>
 +
 
 +
<p> 21+60b_frwrd_premix -- 18..928 of sequence</p>
 +
<p>TATATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTAAGG
 +
ATGATTTCTGGAATTCGCGGCCGCTTCTAGAGCTGATAGCTAGCTCAGTCCTAGGGATTA
 +
TGCTAGCTACTAGAGAAAGAGGAGAAATACTAGATGGCTTCCTCCGAAGACGTTATCAAA
 +
GAGTTCATGCGTTTCAAAGTTCGTATGGAAGGTTCCGTTAACGGTCACGAGTTCGAAATC
 +
GAAGGTGAAGGTGAAGGTCGTCCGTACGAAGGTACCCAGACCGCTAAACTGAAAGTTACC
 +
AAAGGTGGTCCGCTGCCGTTCGCTTGGGACATCCTGTCCCCGCAGTTCCAGTACGGTTCC
 +
AAAGCTTACGTTAAACACCCGGCTGACATCCCGGACTACCTGAAACTGTCCTTCCCGGAA
 +
GGTTTCAAATGGGAACGTGTTATGAACTTCGAAGACGGTGGTGTTGTTACCGTTACCCAG
 +
GACTCCTCCCTGCAAGACGGTGAGTTCATCTACAAAGTTAAACTGCGTGGTACCAACTTC
 +
CCGTCCGACGGTCCGGTTATGCAGAAAAAAACCATGGGTTGGGAAGCTTCCACCGAACGT
 +
ATGTACCCGGAAGACGGTGCTCTGAAAGGTGAAATCAAAATGCGTCTGAAACTGAAAGAC
 +
GGTGGTCACTACGACGCTGAAGTTAAAACCACCTACATGGCTAAAAAACCGGTTCAGCTG
 +
CCGGGTGCTTACAAAACCGACATCAAACTGGACATCACCTCCCACAACGAAGACTACACC
 +
ATCGTTGAACAGTACGAACGTGCTGAAAGGTCGTCACTCCACCGGTGCTTAATAACGCTG
 +
ATAGTGCTAGTGTAGATCGCTACTAGAGATTAAAGAGGAGAAATACTAGTAGCGGCCGCT
 +
GCAGGCTTCCT</p>
 +
 
 +
<h2>Results of J23103 ligation into RFP. Correct but Kirsten says suffix is strange</h2>
 +
 
 +
<p> 21+60c_frwrd_premix -- 18..926 of sequence</p>
 +
<p>AAGATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTAAGG
 +
ATGATTTCTGGAATTCGCGGCCGCTTCTAGAGCTGATAGCTAGCTCAGTCCTAGGGATTA
 +
TGCTAGCTACTAGAGAAAGAGGAGAAATACTAGATGGCTTCCTCCGAAGACGTTATCAAA
 +
GAGTTCATGCGTTTCAAAGTTCGTATGGAAGGTTCCGTTAACGGTCACGAGTTCGAAATC
 +
GAAGGTGAAGGTGAAGGTCGTCCGTACGAAGGTACCCAGACCGCTAAACTGAAAGTTACC
 +
AAAGGTGGTCCGCTGCCGTTCGCTTGGGACATCCTGTCCCCGCAGTTCCAGTACGGTTCC
 +
AAAGCTTACGTTAAACACCCGGCTGACATCCCGGACTACCTGAAACTGTCCTTCCCGGAA
 +
GGTTTCAAATGGGAACGTGTTATGAACTTCGAAGACGGTGGTGTTGTTACCGTTACCCAG
 +
GACTCCTCCCTGCAAGACGGTGAGTTCATCTACAAAGTTAAACTGCGTGGTACCAACTTC
 +
CCGTCCGACGGTCCGGTTATGCAGAAAAAAACCATGGGTTGGGAAGCTTCCACCGAACGT
 +
ATGTACCCGGAAGACGGTGCTCTGAAAGGTGAAATCAAAATGCGTCTGAAACTGAAAGAC
 +
GGTGGTCACTACGACGCTGAAGTTAAAACCACCTACATGGCTAAAAAACCGGTTCAGCTG
 +
CCGGGTGCTTACAAAACCGACATCAAACTGGACATCACCTCCCACAACGAAGACTACACC
 +
ATCGTTGAACAGTACGAACGTGCTGAAGTCGTCACTCCACCGGTGCTTAATAACGCTGAT
 +
AGTGCTAGTGTAGATCGCTACTAGAGATTAAAGAGGAGAAATACTAGTAGCGGCCGCTGC
 +
AGGCTTCCT</p>
 +
 
 +
<h2>Results of corrected SpeI site of sfGFP</h2>
 +
 
 +
<p> sfGFP_reverse_premix -- 20..288 of sequence</p>
 +
<p>ACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCCTGCATAACGCGAAGTAATCTTTTCGGT
 +
TTTAAAGAAAAAGGGCAGGGTGGTGACACCTTGCCCTTTTTTGCCGGACTGCAGCGGCCG
 +
CTACTAGTATCATTACTTATACAGCTCGTCCATACCGTGGGTGATGCCCGCAGCGGTCAC
 +
AAATTCCAGCAGGACCATGTGATCACGTTTTTCGTTCGGATCTTTGCTCAGCACGCTTTG
 +
GGTGCTCAGATAGTGATTATGCCGGAGCC</p>
 +
 
 +
<h2>Sequence of Anti-holin colony 2</h2>
 +
<p> 70.2 F_premix -- 25..943 of sequence</p>
 +
<p>AAAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTAAGG
 +
ATGATTTCTGGAATTCGCGGCCGCTTCTAGAGTTGACGGCTAGCTCAGTCCTAGGTACAG
 +
TGCTAGCCAGCCTGCGGTCCGGGCGATAATAAGGAGGTACTAATGGCCTTAAAAGCAACA
 +
GCACTTTTTGCCATGCTAGGATTGTCATTTGTTTTATCTCCATCGATTGAAGCGAATGTC
 +
GATCCTCATTTTGATAAATTTATGGAATCTGGTATTAGGCACGTTTATATGCTTTTTGAA
 +
AATAAAAGCGTAGAATCGTCTGAACAATTCTATAGTTTTATGAGAACGACCTATAAAAAT
 +
GACCCGTGCTCTTCTGATTTTGAATGTATAGAGCGAGGCGCGGAGATGGCACAATCATAC
 +
GCTAGAATTATGAACATTAAATTGGAGACTGAATAATAACCAGGCATCAAATAAAACGAA
 +
AGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCT
 +
ACTAGAGTCACACTGGCTCACCTTCGGGTGGGCCTTTCTGCGTTTATATACTAGTAGCGG
 +
CCGCTGCAGTCCGGCAAAAAAGGGCAAGGTGTCACCACCCTGCCCTTTTTCTTTAAAACC
 +
GAAAAGATTACTTCGCGTTATGCAGGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGT
 +
TCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATC
 +
AGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAA
 +
AAAGGCCGCGTTGCTGGCGTTTTTCCACAGGCTCCGCCCCCCTGACGAGCATCACAAAAA
 +
TCGACGCTCAAGTCAGAGT</p>
 +
 
 +
<p> 70.2 R_premix -- 19..1016 of sequence</p>
 +
<p>GACGAGCGCAGCGAGTCAGTGAGCGAGGAAGCCTGCATAACGCGAAGTAATCTTTTCGGT
 +
TTTAAAGAAAAAGGGCAGGGTGGTGACACCTTGCCCTTTTTTGCCGGACTGCAGCGGCCG
 +
CTACTAGTATATAAACGCAGAAAGGCCCACCCGAAGGTGAGCCAGTGTGACTCTAGTAGA
 +
GAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTC
 +
GTTTTATTTGATGCCTGGTTATTATTCAGTCTCCAATTTAATGTTCATAATTCTAGCGTA
 +
TGATTGTGCCATCTCCGCGCCTCGCTCTATACATTCAAAATCAGAAGAGCACGGGTCATT
 +
TTTATAGGTCGTTCTCATAAAACTATAGAATTGTTCAGACGATTCTACGCTTTTATTTTC
 +
AAAAAGCATATAAACGTGCCTAATACCAGATTCCATAAATTTATCAAAATGAGGATCGAC
 +
ATTCGCTTCAATCGATGGAGATAAAACAAATGACAATCCTAGCATGGCAAAAAGTGCTGT
 +
TGCTTTTAAGGCCATTAGTACCTCCTTATTATCGCCCGGACCGCAGGCTGGCTAGCACTG
 +
TACCTAGGACTGAGCTAGCCGTCAACTCTAGAAGCGGCCGCGAATTCCAGAAATCATCCT
 +
TAGCGAAAGCTAAGGATTTTTTTTATCTGAAATTCTGCCTCGTGATACGCCTATTTTTAT
 +
AGGTTAATGTCATGATAATAATGGTTTCTTAGACGTCAGGTGGCACTCGAGTTGATCGGG
 +
CACGTAAGAGGTTCCAACTTTCACCATAATGAAATAAGATCACTACCGGGCGTATTTTTT
 +
GAGTTATCGAGATTTTCAGGAGCTAAGGAAGCTAAAATGGAGAAAAAAATCACTGGATAT
 +
ACCACCGTTGATATATCCCAATGGCATCGTAAAGAACATTTTGAGGCATTTCAGTCAGTT
 +
GCTCAATGTACCTATAACCAGACCGTTCAGCTGGATAT</p>
 +
 
 +
<h2>Sequence of Anti-holin colony 2</h2>
 +
 
 +
<p> 70.9 F_premix -- 18..1008 of sequence</p>
 +
<p>ATAAAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTAA
 +
GGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGTTGACGGCTAGCTCAGTCCTAGGTAC
 +
AGTGCTAGCCAGCCTGCGGTCCGGGCGATAATAAGGAGGTACTAATGGCCTTAAAAACAA
 +
CAGCACTTTGGCACAATCATACGCTAGAATTATGAACATTAAATTGGAGACTGAATAATA
 +
ACCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGT
 +
TGTTTGTCGGTGAACGCTCTCTACTAGAGTCACACTGGCTCACCTTCGGGTGGGCCTTTC
 +
TGCGTTTATATACTAGTAGCGGCCGCTGCAGTCCGGCAAAAAAGGGCAAGGTGTCACCAC
 +
CCTGCCCTTTTTCTTTAAAACCGAAAAGATTACTTCGCGTTATGCAGGCTTCCTCGCTCA
 +
CTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGG
 +
TAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCC
 +
AGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCACAGGCTCCGCC
 +
CCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGAC
 +
TATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCC
 +
TGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATA
 +
GCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGC
 +
ACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCA
 +
ACCCGGTAAGACACGACTTATCGCCACTGGC</p>
 +
 
 +
<p> 70.9 R_premix -- 21..998 of sequence</p>
 +
<p>ACGAGCGCAGCGAGTCAGTGAGCGAGGAAGCCTGCATAACGCGAAGTAATCTTTTCGGTT
 +
TTAAAGAAAAAGGGCAGGGTGGTGACACCTTGCCCTTTTTTGCCGGACTGCAGCGGCCGC
 +
TACTAGTATATAAACGCAGAAAGGCCCACCCGAAGGTGAGCCAGTGTGACTCTAGTAGAG
 +
AGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCG
 +
TTTTATTTGATGCCTGGTTATTATTCAGTCTCCAATTTAATGTTCATAATTCTAGCGTAT
 +
GATTGTGCCAAAGTGCTGTTGTTTTTAAGGCCATTAGTACCTCCTTATTATCGCCCGGAC
 +
CGCAGGCTGGCTAGCACTGTACCTAGGACTGAGCTAGCCGTCAACTCTAGAAGCGGCCGC
 +
GAATTCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATTCTGCCTC
 +
GTGATACGCCTATTTTTATAGGTTAATGTCATGATAATAATGGTTTCTTAGACGTCAGGT
 +
GGCACTCGAGTTGATCGGGCACGTAAGAGGTTCCAACTTTCACCATAATGAAATAAGATC
 +
ACTACCGGGCGTATTTTTTGAGTTATCGAGATTTTCAGGAGCTAAGGAAGCTAAAATGGA
 +
GAAAAAAATCACTGGATATACCACCGTTGATATATCCCAATGGCATCGTAAAGAACATTT
 +
TGAGGCATTTCAGTCAGTTGCTCAATGTACCTATAACCAGACCGTTCAGCTGGATATTAC
 +
GGCCTTTTTAAAGACCGTAAAGAAAAATAAGCACAAGTTTTATCCGGCCTTTATTCACAT
 +
TCTTGCCCGCCTGATGAATGCTCATCCGGAATTTCGTATGGCAATGAAAGACGGTGAGCT
 +
GGTGATATGGGATAGTGTTCACCCTTGTTACACCGTTTTCCATGAGCAAACTGAAACGTT
 +
TTCATCGCTCTGGAGTGA</p>
 +
 
 +
<h2>Sequence of Dendra2 construct. Verified.</h2>
 +
 
 +
<p> 71.2F_premix -- 14..961 of sequence
 +
ATAAAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTAA
 +
GGATGATTTCTGGAATTCGCGGCCGCTTCTAGATGAACCTCATCAAAGAGGATATGCGCG
 +
TAAAAGTCCACATGGAAGGCAACGTGAATGGTCATGCGTTCGTAATCGAAGGTGAAGGCA
 +
AAGGGAAACCGTACGAAGGTACGCAAACTGCAAACTTGACCGTGAAAGAAGGTGCACCTC
 +
TGCCATTCAGCTACGACATTCTTACCACTGCGGTTCACTATGGCAATCGTGTCTTTACGA
 +
AATATCCGGAAGATATTCCGGACTACTTCAAACAGTCGTTTCCGGAAGGCTATTCATGGG
 +
AGCGTACCATGACCTTTGAGGATAAAGGCATTTGCACAATTCGCAGCGATATTAGCCTGG
 +
AAGGGGACTGCTTCTTTCAGAACGTTCGGTTTAAAGGCACGAATTTTCCTCCGAATGGGC
 +
CGGTTATGCAGAAGAAAACCCTGAAATGGGAACCGTCGACAGAGAAACTGCATGTGCGTG
 +
ATGGACTTCTGGTCGGTAACATCAACATGGCCTTACTGTTGGAAGGTGGAGGCCATTATC
 +
TCTGTGACTTCAAAACCACGTATAAGGCGAAGAAAGTGGTACAGCTGCCAGATGCCCACT
 +
TTGTGGATCATCGCATCGAAATTCTGGGCAATGACTCCGATTACAACAAAGTCAAGCTGT
 +
ACGAGCATGCTGTTGCTCGCTATAGTCCCTTACCCTCTCAAGTGTGGTGATAATACTAGT
 +
AGCGGCCGCTGCAGTCCGGCAAAAAAACGGCAAGGTGTCACCACCCTGCCCTTTTTCTTT
 +
AAAACCGAAAAGATTACTTCGCGTTATGCAGGCTTCCTCGCTCACTGACTCGCTGCGCTC
 +
GGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAAT</p>
 +
 
 +
<p> 71.2R_premix -- 16..995 of sequence
 +
GACGAGCGCAGCGAGTCAGTGAGCGAGGAAGCCTGCATAACGCGAAGTAATCTTTTCGGT
 +
TTTAAAGAAAAAGGGCAGGGTGGTGACACCTTGCCGTTTTTTTGCCGGACTGCAGCGGCC
 +
GCTACTAGTATTATCACCACACTTGAGAGGGTAAGGGACTATAGCGAGCAACAGCATGCT
 +
CGTACAGCTTGACTTTGTTGTAATCGGAGTCATTGCCCAGAATTTCGATGCGATGATCCA
 +
CAAAGTGGGCATCTGGCAGCTGTACCACTTTCTTCGCCTTATACGTGGTTTTGAAGTCAC
 +
AGAGATAATGGCCTCCACCTTCCAACAGTAAGGCCATGTTGATGTTACCGACCAGAAGTC
 +
CATCACGCACATGCAGTTTCTCTGTCGACGGTTCCCATTTCAGGGTTTTCTTCTGCATAA
 +
CCGGCCCATTCGGAGGAAAATTCGTGCCTTTAAACCGAACGTTCTGAAAGAAGCAGTCCC
 +
CTTCCAGGCTAATATCGCTGCGAATTGTGCAAATGCCTTTATCCTCAAAGGTCATGGTAC
 +
GCTCCCATGAATAGCCTTCCGGAAACGACTGTTTGAAGTAGTCCGGAATATCTTCCGGAT
 +
ATTTCGTAAAGACACGATTGCCATAGTGAACCGCAGTGGTAAGAATGTCGTAGCTGAATG
 +
GCAGAGGTGCACCTTCTTTCACGGTCAAGTTTGCAGTTTGCGTACCTTCGTACGGTTTCC
 +
CTTTGCCTTCACCTTCGATTACGAACGCATGACCATTCACGTTGCCTTCCATGTGGACTT
 +
TTACGCGCATATCCTCTTTGATGAGGTTCATCTAGAAGCGGCCGCGAATTCCAGAAATCA
 +
TCCTTAGCGAAAGCTAAGGATTTTTTTTTATCTGAAATTCTGCCTCGTGATACGCCTATT
 +
TTTATAGGTTAATGTCATGATAATAATGGTTTCTTAGACGTCAGTGGCACTCGAGTTGAT
 +
CGGGCACGTAAGAGGTTCCA</p>
 +
 
 +
<h2>Ptet-Dendra2 composite sequencing results. Sequencing results were bad probably due to low concentration of DNA being sent off. One possible base pair mutation and 7% of sequence was not covered.</h2>
 +
 
 +
<p> 72F_premix -- 15..135 of sequence
 +
TAAAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTAAG
 +
GATGATTTCTGGAATTCGCGGCCGCTTCTAGAGTCCCTATCAGTGATAGAGATACTGATC
 +
C</p>
 +
 
 +
<p>72R_premix -- 20..851 of sequence
 +
ACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCCTGCATAACGCGAAGTAATCTTTTCGGT
 +
TTTAAAGAAAAAGGGCAGGGTGGTGACACCTTGCCGTTTTTTTGCCGGACTGCAGCGGCC
 +
GCTACTAGTATTATCACCACACTTGAGAGGGTAAGGGACTATAGCGAGCAACAGCATGCT
 +
CGTACAGCTTGACTTTGTTGTAATCGGAGTCATTGCCCAGAATTTCGATGCGATGATCCA
 +
CAAAGTGGGCATCTGGCAGCTGTACCACTTTCTTCGCCTTATACGTGGTTTTGAAGTCAC
 +
AGAGATAATGGCCTCCACCTTCCAACAGTAAGGCCATGTTGATGTTACCGACCAGAAGTC
 +
CATCACGCACATGCAGTTTCTCTGTCGACGGTTCCCATTTCAGGGTTTTCTTCTGCATAA
 +
CCGGCCCATTCGGAGGAAAATTCGTGCCTTTAAACCGAACGTTCTGAAAGAAGCAGTCCC
 +
CTTCCAGGCTAATATCGCTGCGAATTGTGCAAATGCCTTTATCCTCAAAGGTCATGGTAC
 +
GCTCCCATGAATAGCCTTCCGGAAACGACTGTTTGAAGTAGTCCGGAATATCTTCCGGAT
 +
ATTTCGTAAAGACACGATTGCCATAGTGAACCGCAGTGGTAAGAATGTCGTAGCTGAATG
 +
GCAGAGGTGCACCTTCTTTCACGGTCAAGTTTGCAGTTTGCGTACCTTCGTACGGTTTCC
 +
CTTTGCCTTCACCTTCGATTACGAACGCATGACCATTCACGTTGCCTTCCATGTGGACTT
 +
TTACGCGCATATCCTCTTTGATGAGGTTCATCTAGTATTGCTCAGCATCTCT</p>
 +
 
 +
<h2>J23100-sfGFP composite. Sequence verified.</h2>
 +
 
 +
 
 +
<p> 73F_premix -- 22..1003 of sequence
 +
AAAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTAAGG
 +
ATGATTTCTGGAATTCGCGGCCGCTTCTAGAGTTGACGGCTAGCTCAGTCCTAGGTACAG
 +
TGCTAGCTACTAGAGAAATCAAATTAAGGAGGTAAGATAATGCGTAAAGGCGAAGAACTG
 +
TTCACGGGCGTAGTTCCGATTCTGGTCGAGCTGGACGGCGATGTGAACGGTCATAAGTTT
 +
AGCGTTCGCGGTGAAGGTGAGGGCGACGCGACCAACGGCAAACTGACCCTGAAGTTCATC
 +
TGCACCACCGGTAAACTGCCGGTGCCTTGGCCGACCTTGGTGACGACGTTGACGTATGGC
 +
GTGCAGTGTTTTGCGCGTTATCCGGACCACATGAAACAACACGATTTCTTCAAATCTGCG
 +
ATGCCGGAGGGTTACGTCCAGGAGCGTACCATTTCCTTCAAGGATGATGGCTACTACAAA
 +
ACTCGCGCAGAGGTTAAGTTTGAAGGTGACACGCTGGTCAATCGTATCGAATTGAAGGGT
 +
ATCGACTTTAAAGAGGATGGTAACATTCTGGGCCATAAACTGGAGTATAACTTCAACAGC
 +
CATAATGTTTACATTACGGCAGACAAGCAAAAGAACGGCATCAAGGCCAATTTCAAGATT
 +
CGCCACAATGTTGAGGACGGTAGCGTCCAACTGGCCGACCATTACCAGCAGAACACCCCA
 +
ATTGGTGACGGTCCGGTTTTGCTGCCGGATAATCACTATCTGAGCACCCAAAGCGTGCTG
 +
AGCAAAGATCCGAACGAAAAACGTGATCACATGGTCCTGCTGGAATTTGTGACCGCTGCG
 +
GGCATCACCCACGGTATGGACGAGCTGTATAAGTAATGATACTAGTAGCGGCCGCTGCAG
 +
TCCGGCAAAAAAGGGCAAGGTGTCACCACCCTGCCCTTTTTCTTTAAAACCGAAAAGATT
 +
ACTTCGCGTTATGCAGGCTTCC</p>
 +
 
 +
<p> 73R_premix -- 19..338 of sequence
 +
GACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCCTGCATAACGCGAAGTAATCTTTTCGG
 +
TTTTAAAGAAAAAGGGCAGGGTGGTGACACCTTGCCCTTTTTTGCCGGACTGCAGCGGCC
 +
GCTACTAGTATCATTACTTATACAGCTCGTCCATACCGTGGGTGATGCCCGCAGCGGTCA
 +
CAAATTCCAGCAGGACCATGTGATCACGTTTTTCGTTCGGATCTTTGCTCAGCACGCTTT
 +
GGGTGCTCAGATAGTGATTATCCGGCAGCAAAACCGGACCGTCACCAATTGGGGTGTTCT
 +
GCTGGTAATGGTCGGGTTAC</p>
 +
 
 +
 
 +
 
</html>
</html>

Latest revision as of 09:52, 20 September 2011



Sequencing Results

Vector 7 forward sequencing result

BBa_K316001 (7)_premix -- 19..973 of sequence

TATAAAAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCT AAGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGAATTTTGTCAAAATAATTTTATTG ACAACGTCTTATTAACGTTGATATAATTTAAATTTTATTTGACAAAAATGGGCTCGTGTT GTACAATAAATGTTACTAGTAGCGGCCGCTGCAGTCCGGCAAAAAAACGGCAAGGTGTCA CCACCCTGCCCTTTTTCTTTAAAACCGAAAAGATTACTTCGCGTTATGCAGGCTTCCTCG CTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAG GCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAA GGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCACAGGCTC CGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACA GGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCG ACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCT CATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGT GTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAG TCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGGTAACAGGATTAG CAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTA CACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGA

Vector 8 forward sequencing result

BBa_K316005 (8)_premix -- 17..635 of sequence

TAGAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTAAG GATGATTTCTGGAATTCGCGGCCGCTTCTAGAGAATTTTGTCAAAATAATTTTATTGACA ACGTCTTATTAACGTTGATATAATTTAAATTTTTTTTGACAAAAATGGGCTCGTGTTGTA CAATAAATGTTACTAGAGCTCATGAACTATGAAGAGGTGACGTCATGAACAAAGGTGTAA TGCGACCGGGCCATGTGCAGCTGCGTGTACTGGACATGAGCAAGGCCCTGGAACACTACG TCGAGTTGCTGGGCCTGATCGAGATGGACCGTGACGACCAGGGCCGTGTCTATCTGAAGG CTTGGACCGAAGTGGATAAGTTTTCCCTGGTGCTACGCGAGGCTGACGAGCCGGGCATGG ATTTTATGGGTTTCAAGGTTGTGGATGAGGATGCTCTCCGGCAACTGGAGCGGGATCTGA TGGCATATGGCTGTGCCGTTGAGCAGCTACCCGCAGGTGAACTGAACAGTTGTGGCCGGC GCGTGCGCTTCCAGGCCCCCTCCGGGCATCACTTCGAGTTGTATGCAGACAAGGAATATA CTGGAAAGTGGGGTTTGAA

Vector 6 forward sequencing result

BBa_K398500 (6)_premix -- 17..960 of sequence

TATAAAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTA AGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGTTGACGGCTAGCTCAGTCCTAGGTA CAGTGCTAGCTTCTAGAGAAAGAGGGGACAAACTAGATGCGTAAAGGAGAAGAACTTTTC ACTGGAGTTGTCCCAATTCTTGTTGAATTAGATGGTGATGTTAATGGGCACAAATTTTCT GTCAGTGGAGAGGGTGAAGGTGATGCAACATACGGAAAACTTACCCTTAAATTTATTTGC ACTACTGGAAAACTACCTGTTCCATGGCCAACACTTGTCACTACTTTCGGTTATGGTGTT CAATGCTTTGCGAGATACCCAGATCATATGAAACAGCATGACTTTTTCAAGAGTGCCATG CCCGAAGGTTATGTACAGGAAAGAACTATATTTTTCAAAGATGACGGGAACTACAAGACA CGTGCTGAAGTCAAGTTTGAAGGTGATACCCTTGTTAATAGAATCGAGTTAAAAGGTATT GATTTTAAAGAAGATGGAAACATTCTTGGACACAAATTGGAATACAACTATAACTCACAC AATGTATACATCATGGCAGACAAACAAAAGAATGGAATCAAAGTTAACTTCAAAATTAGA CACAACATTGAAGATGGAAGCGTTCAACTAGCAGACCATTATCAACAAAATACTCCAATT GGCGATGGCCCTGTCCTTTTACCAGACAACCATTACCTGTCCACACAATCTGCCCTTTCG AAAGATCCCAACGAAAAGAGAGACCACATGGTCCTTCTTGAGTTTGTAACAGCTGCTGGG ATTACACATGGCATGGATGAACTATACAAATAATAATACTAGAGCCAGGCATCAAATAAA ACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTT

?

PSVi34-1_premix -- 19..951 of sequence

CATGAATGTCTTTGTACGAAACTCATTACCCGCATTAATATTTTTTTTCTTTTTAAAGCT CAGTTGACCCTTTCTCATTCCCTTCTTAAAACAACTGTGTGATCCTTGAGAAAAGATAAA TTACATACACAACATAAACCCAACTACTAGTTATTAAAATGTCTAAAGGTGAAGAATTAT TCACTGGTGTTGTCCCAATTTTGGTTGAATTAGATGGTGATGTTAATGGTCACAAATTTT CTGTCTCCGGTGAAGGTGAAGGTGATGCTACTTACGGTAAATTGACCTTAAAATTTATTT GTACTACTGGTAAATTGCCAGTTCCATGGCCAACCTTAGTCACTACTTTCGGTTATGGTG TTCAATGTTTTGCGAGATACCCAGATCATATGAAACAACATGACTTTTTCAAGTCTGCCA TGCCAGAAGGTTATGTTCAAGAAAGAACTATTTTTTTCAAAGATGACGGTAACTACAAGA CCAGAGCTGAAGTCAAGTTTGAAGGTGATACCTTAGTTAATAGAATCGAATTAAAAGGTA TTGATTTTAAAGAAGATGGTAACATTTTAGGTCACAAATTGGAATACAACTATAACTCTC ACAATGTTTACATCATGGCTGACAAACAAAAGAATGGTATCAAAGTTAACTTCAAAATTA GACACAACATTGAAGATGGTTCTGTTCAATTAGCTGACCATTATCAACAAAATACTCCAA TTGGTGATGGTCCAGTCTTGTTACCAGACAACCATTACTTATCCACTCAATCTGCCTTAT CCAAAGATCCAAACGAAAAGAGAGACCACATGGTCTTGTTAGAATTTGTTACTGCTGCTG GTATTACCCATGGTATGGATGAATTGTACAAATAACTCGAGTAAGCTTGGTACCGCGGCT AGCTAAGATCCGCTCTAACCGAAAAGGAAGGAG

?

PSVi34-2_premix -- 21..949 of sequence

ATGAATGTCTTTGTACGAAACTCATTACCCGCATTAATATTTTTTTTCTTTTTAAAGCTC AGTTGACCCTTTCTCATTCCCTTCTTAAAACAACTGTGTGATCCTTGAGAAAAGATAAAT TACATACACAACATAAACCCAACTACTAGTTATTAAAATGTCTAAAGGTGAAGAATTATT CACTGGTGTTGTCCCAATTTTGGTTGAATTAGATGGTGATGTTAATGGTCACAAATTTTC TGTCTCCGGTGAAGGTGAAGGTGATGCTACTTACGGTAAATTGACCTTAAAATTTATTTG TACTACTGGTAAATTGCCAGTTCCATGGCCAACCTTAGTCACTACTTTCGGTTATGGTGT TCAATGTTTTGCGAGATACCCAGATCATATGAAACAACATGACTTTTTCAAGTCTGCCAT GCCAGAAGGTTATGTTCAAGAAAGAACTATTTTTTTCAAAGATGACGGTAACTACAAGAC CAGAGCTGAAGTCAAGTTTGAAGGTGATACCTTAGTTAATAGAATCGAATTAAAAGGTAT TGATTTTAAAGAAGATGGTAACATTTTAGGTCACAAATTGGAATACAACTATAACTCTCA CAATGTTTACATCATGGCTGACAAACAAAAGAATGGTATCAAAGTTAACTTCAAAATTAG ACACAACATTGAAGATGGTTCTGTTCAATTAGCTGACCATTATCAACAAAATACTCCAAT TGGTGATGGTCCAGTCTTGTTACCAGACAACCATTACTTATCCACTCAATCTGCCTTATC CAAAGATCCAAACGAAAAGAGAGACCACATGGTCTTGTTAGAATTTGTTACTGCTGCTGG TATTACCCATGGTATGGATGAATTGTACAAATAACTCGAGTAAGCTTGGTACCGCGGCTA GCTAAGATCCGCTCTAACCGAAAAGGAAG

Sequence results for J23113 ligation attempt into RFP. Failed.

52.E_premix -- 14..957 of sequence

ATAAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTAAG GATGATTTCTGGAATTCGCGGCCGCTTCTAGAGAAAGAGGAGAAATACTAGATGGCTTCC TCCGAAGACGTTATCAAAGAGTTCATGCGTTTCAAAGTTCGTATGGAAGGTTCCGTTAAC GGTCACGAGTTCGAAATCGAAGGTGAAGGTGAAGGTCGTCCGTACGAAGGTACCCAGACC GCTAAACTGAAAGTTACCAAAGGTGGTCCGCTGCCGTTCGCTTGGGACATCCTGTCCCCG CAGTTCCAGTACGGTTCCAAAGCTTACGTTAAACACCCGGCTGACATCCCGGACTACCTG AAACTGTCCTTCCCGGAAGGTTTCAAATGGGAACGTGTTATGAACTTCGAAGACGGTGGT GTTGTTACCGTTACCCAGGACTCCTCCCTGCAAGACGGTGAGTTCATCTACAAAGTTAAA CTGCGTGGTACCAACTTCCCGTCCGACGGTCCGGTTATGCAGAAAAAAACCATGGGTTGG GAAGCTTCCACCGAACGTATGTACCCGGAAGACGGTGCTCTGAAAGGTGAAATCAAAATG CGTCTGAAACTGAAAGACGGTGGTCACTACGACGCTGAAGTTAAAACCACCTACATGGCT AAAAAACCGGTTCAGCTGCCGGGTGCTTACAAAACCGACATCAAACTGGACATCACCTCC CACAACGAAGACTACACCATCGTTGAACAGTACGAACGTGCTGAAGGTCGTCACTCCACC GGTGCTTAATAACGCTGATAGTGCTAGTGTAGATCGCTACTAGAGATTAAAGAGGAGAAA TACTAGTAGCGGCCGCTGCAGGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGG CTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTT

Sequence results for possible auxin producing colony

53_AUX1 -- 13..914 of sequence

GCATCTGATTTTCCAATAGTATGTTAGAAATCTTTCGTGTTGTGATGACGAATTGTGATG AGCACCAGCATCTGATTGTTGGTGGCGTACAGCAAGTGCCTGTAGGCCTGTGGTCGCACG TGCCTGAACGCTGCGCACATTGGCCAAAGGGTACGTCTCTTTCCTCACTTCATCGGGGTG CCCCGCGCCCAGGTGTCAAACGTATTGCACGTGCTGAAGATGGTTCATTTACGGTTACAG ATAATTGGGGCGATACACGTCAATACGCTGCCGTTTTGACAACCTGTCAATCGTGGCTGC TTACAACGCAGATTGAATGTGAGGAATCTTTGTTTTCACAGAAGATGTGGATGGCGCTGG ATCGCACGCGTTACATGCAGTCTTCTAAAACTTTTGTGATGGTTGATCGGCCGTTTTGGA AGGACAAAGATCCGGAAACGGGTCGCGATCTTATGTCCATGACCCTCACCGATCGCTTAA CGCGCGGCACATACTTATTTGATAATGGCGACGACAAGCCAGGCGTTATTTGCCTGAGTT ACTCATGGATGAGTGACGCTTTAAAAATGTTACCGCAACCGATCGAGAAACGCGTCAAAT TAGCTTTAGATGCCCTTAAAAAGATTTACCCGAAAGTGGATATCGCCGCACGTATTATTG GTGACCCGATTACTGTGAGTTGGGAAGCGGATCCGCATTTCCTGGGGGCATTTAAAGGCG CGTTACCGGGCCATTACCGTTACAACCAGCGCATGTACGCGCATTTTATGCAGCAGACAT GCCGTCAGAACAGCGTGGCATGTTTATTGCCGGTGATGATGTATCTTGGACACCGGCATG GGTTGAGGGAGCAGTACAAACGTCATTGAACGCGGTGTGGGGCATCATGAACCATTTCGG AG

53_AUX2 -- 16..895 of sequence

GGACAGTACAACGTCATTGAACGCGGTGTGGGGCATCATGAACCATTTCGGAGGGAAAAC CCACGCAGAGAATCCGGGTCCTGGCGACGTTTTCCATGAGATTGGACCGATCGCGCTGGG GGACTAATAATCGCTGGGACGCCCGCCCCTAGAAAAGGAGGACTAAAAATGCGCGAAATG ATTACGCTTGAATCTCTGTGCCGCGCATTGGCAGACGAACAGATTGCCGCAGAAGAATTG CGCGAGCGCGCGCTGGATACAGAGGCTCGCTTAACACTGTTAAATTGCTTCATTCGCGAA GGCGACGCCGTAAGCCAATTCGGTGAAGCGGATCAGGCGCGTAAAGGAACTTCACTTTGG GGGGTGCCTGTGAGTTTTAAGGATAACATCTGTGTTCGCGGGCTGCCACTGACGGCGGGT ACCCGCGGCATGAGCGGCTTTATTGCCGATCAGGATGCAGCAATCGTGAGCCAGTTAAAA GCATTGGGAGCTGTGGTCGCGGGCAAAAATAACATGCATGAGCTGTCATTTGGCGTCACG AGCATCAATCCACATTGGGGCGCGGTTGGAAACCCGGTGGCTCCAGGATATTGCGCCGGA GGTTCTAGCGGAGGTTCCGCTGCGGCGGTCGCATCTGGAATTGTCCCGCTCAGCGTCGGG ACGGACACAGGCGGCTCAATCCGTATTCCGGCGGCATTTTGCGGCATTACGGGTTTTCGC CCTACTACAGGTCGCTTGAGTACGGCGGGGATCATTCCGGTCAGCCACACGAAAGGATTG TGTCGGCCTCCTGACGCGCACAGCTGGCGACGCTGAATTTGTGTATGGGCTGCTGTCAGA AAACAGCAGAGTTTCCCTCTTAATCGCACGGGACCTTGCG

53_AUX3 -- 15..793 of sequence

GATGATGTGTCGGCCTCCTGACGCGCACAGCTGGCGACGCTGAATTTGTGTATGGGCTGC TGTCAGGAAAACAGCAGAGTTTCCCTCTTAATCGCACGGGACCTTGCCGCATCGGCCTGC CGGTGAGCATGTGGTCTGACTTAGATGGCGAAGTAGAACGTGCCTGTATCAATGCGCTGA GCCTCTTACGCAAAACGGGCTTTGAATTTGTTGAGATCGACGATGCGGACATTGTGGAAT TGAACCAGACATTAACCTTCACGGTGCCGCTGTACGAATTTTTCGCGGATTTTGCTCAAT CCTTGTTGTCCTTAGGTTGGAAACATGGCATCCATCATATTTTTGCACAAGTGGATGATG CAAATGTGAAAGGCATTATTAATCACCATCTGGGAGAGGGCGCGATTAAGCCGGCACATT ATCTGAGTTCTCTCCAGAATGGAGAGCTGCTGAAACGCAAGATGGATGAGCTTTTTGCGC GCCATCACATTAAACTTTTAGGGTATCCGACGGTCCCGTGTCGCGTGCCTCATCTGGACC ATGCAGATCGTCCGGAATTTTTTAGCCAGGCCATCCGTAACACAGACCTCGCAAGCAACG CGATGTTACCAAGCATTACAATTCCGGTCGGTCCGGAAGGCCGCCTGCCGGTCGGCCTTA GTTTTGATGCGCCTCGTGCTCGTGATGCGTTTCTCTTAAGCAATGTTAGTTTGATCGAAA AAGTGTTAAAAGGCTAATAATACTAGTAGCGGCCGCTGCAGTCCGGCAAAAAAGGGCAA

53_premix -- 17..119 of sequence

GACGAGCGCAGCGAGTCAGTGAGCGAGGAAGCCTGCATAACGCGAAGTAATCTTTTCGGT TTTAAAGAAAAAGGGCAGGGTGGTGACACCTTGCCCTTTTTTG

53_premix -- 15..843 of sequence

ATATATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTAAG GATGATTTCTGGAATTCGCGGCCGCTTCTAGAGAATTTTGTCAAAATAATTTTATTGACA ACGTCTTATTAACGTTGATATAATTTAAATTTTTTTTGACAAAAATGGGCTCGTGTTGTA CAATAAATGTCAGCCTGCGGTCCGGTGGCCTTAACTACCAACTCACCGGAGGGTATATAT GTTTGGACCGGATTTCCCGTTTGCATTTGATGATTGGATCGAACATCCTAAAGGACTTGG GTCAATTCCGGCAGAAAATCACGGCGCTGAGGTTGCAATTATCGGGGCCGGCATTGCAGG CTTAGTAGCAGCATATGAATTAATGAAGATGGGTCTGAAACCTGTAGTCTATGAGGCCTC CAAAATGGGAGGTCGCTTACGGTCACAGGAATTTGAAGGAGCGAAGGGAATTGTTGCGGA ACTGGGCGGTATGCGCTTTCCAGTCTCCTCCACAGCGTTCTTTCATTACGTTGATAAACT GGGCCTTGAAAGTCGTCCGTTCCCTAACCCTTTGACGGCGGCCAGTGGCAGCACGGTGAT TGACCTGGAAGGTACCACGTACTATGCACAGATGTTATCAGATCTCCCAGCCCTGTTCCA AGAAGTGGCGGATGCATGGGCAGACGCCTTAGAATCAGGCTCTCAATTTGGAGATATTCA ACAGGCCATTCGTGATTCTGACGTACCGCGTCTCAAGGGAACTGTGGACCAAACTCGTGC CTTTGTGGGACGATCGCACTTTCTATGACTTTGTAGCGACCTCAAAGGC

Sequence results for possible auxin producing colony

55_AUX1 -- 18..841 of sequence

ATTTTCAATAGTATGTTAGAAATCTTTCGTGTTGTGATGACGAATTGTGATGAGCACCAG CATCTGATTGTTGGTGGCGTACAGCAAGTGCCTGTAGGCCTGTGGTCGCACGTGCCTGAA CGCTGCGCACATTGGCCAAAGGGTACGTCTCTTTCCTCACTTCATCGGGGTGCCCCGCGC CCAGGTGTCAAACGTATTGCACGTGCTGAAGATGGTTCATTTACGGTTACAGATAATTGG GGCGATACACGTCAATACGCTGCCGTTTTGACAACCTGTCAATCGTGGCTGCTTACAACG CAGATTGAATGTGAGGAATCTTTGTTTTCACAGAAGATGTGGATGGCGCTGGATCGCACG CGTTACATGCAGTCTTCTAAAACTTTTGTGATGGTTGATCGGCCGTTTTGGAAGGACAAA GATCCGGAAACGGGTCGCGATCTTATGTCCATGACCCTCACCGATCGCTTAACGCGCGGC ACATACTTATTTGATAATGGCGACGACAAGCCAGGCGTTATTTGCCTGAGTTACTCATGG ATGAGTGACGCTTTAAAAATGTTACCGCAACCGATCGAGAAACGCGTCAAATTAGCTTTA GATGCCCTTAAAAAGATTTACCCGAAAGTGGATATCGCCGCACGTATTATTGGTGACCCG ATTACTGTGAGTTGGGAAGCGGATCCGCATTTCCTGGGGGCATTTAAAGGCGCGTTACCG GGCCATTACCGTTACAACCAGCGCATGTACGCGCATTTTATGCAGCAGGACATGCCGTCA GAACAGCGTGGCATGTTTATTGCCGGTGATGATGTATCTTGACA

55_AUX2 -- 16..893 of sequence

CAGTACAACGTCATTGAACGCGGTGTGGGGCATCATGAACCATTTCGGAGGGAAAACCCA CGCAGAGAATCCGGGTCCTGGCGACGTTTTCCATGAGATTGGACCGATCGCGCTGGGGGA CTAATAATCGCTGGGACGCCCGCCCCTAGAAAAGGAGGACTAAAAATGCGCGAAATGATT ACGCTTGAATCTCTGTGCCGCGCATTGGCAGACGAACAGATTGCCGCAGAAGAATTGCGC GAGCGCGCGCTGGATACAGAGGCTCGCTTAACACTGTTAAATTGCTTCATTCGCGAAGGC GACGCCGTAAGCCAATTCGGTGAAGCGGATCAGGCGCGTAAAGGAACTTCACTTTGGGGG GTGCCTGTGAGTTTTAAGGATAACATCTGTGTTCGCGGGCTGCCACTGACGGCGGGTACC CGCGGCATGAGCGGCTTTATTGCCGATCAGGATGCAGCAATCGTGAGCCAGTTAAAAGCA TTGGGAGCTGTGGTCGCGGGCAAAAATAACATGCATGAGCTGTCATTTGGCGTCACGAGC ATCAATCCACATTGGGGCGCGGTTGGAAACCCGGTGGCTCCAGGATATTGCGCCGGAGGT TCTAGCGGAGGTTCCGCTGCGGCGGTCGCATCTGGAATTGTCCCGCTCAGCGTCGGGACG GACACAGGCGGCTCAATCCGTATTCCGGCGGCATTTTGCGGCATTACGGGTTTTCGCCCT ACTACAGGTCGCTTGAGTACGGCGGGGATCATTCCGGTCAGCCACACGAAAGGATTGTGT CGGCCTCCTGACGCGCACAGCTGGCGACGCTGAATTTGTGTATGGGCTGCTGTCAGGAAA ACAGCAGAGTTTCCCTCTTAATCGCACGGGACCTTGCG

55_AUX3 -- 13..851 of sequence

GATGATGTGTCGGCCTCCTGACGCGCACAGCTGGCGACGCTGAATTTGTGTATGGGCTGC TGTCAGGAAAACAGCAGAGTTTCCCTCTTAATCGCACGGGACCTTGCCGCATCGGCCTGC CGGTGAGCATGTGGTCTGACTTAGATGGCGAAGTAGAACGTGCCTGTATCAATGCGCTGA GCCTCTTACGCAAAACGGGCTTTGAATTTGTTGAGATCGACGATGCGGACATTGTGGAAT TGAACCAGACATTAACCTTCACGGTGCCGCTGTACGAATTTTTCGCGGATTTTGCTCAAT CCTTGTTGTCCTTAGGTTGGAAACATGGCATCCATCATATTTTTGCACAAGTGGATGATG CAAATGTGAAAGGCATTATTAATCACCATCTGGGAGAGGGCGCGATTAAGCCGGCACATT ATCTGAGTTCTCTCCAGAATGGAGAGCTGCTGAAACGCAAGATGGATGAGCTTTTTGCGC GCCATCACATTAAACTTTTAGGGTATCCGACGGTCCCGTGTCGCGTGCCTCATCTGGACC ATGCAGATCGTCCGGAATTTTTTAGCCAGGCCATCCGTAACACAGACCTCGCAAGCAACG CGATGTTACCAAGCATTACAATTCCGGTCGGTCCGGAAGGCCGCCTGCCGGTCGGCCTTA GTTTTGATGCGCCTCGTGCTCGTGATGCGTTTCTCTTAAGCAATGTTAGTTTGATCGAAA AAGTGTTAAAAGGCTAATAATACTAGTAGCGGCCGCTGCAGTCCGGCAAAAAAACGGCAA GGTGTCACCACCCTGCCCTTTTTCTTTAAAACCGAAAAGATTACTTCGCGTTATGCAGC

55_premix -- 16..869 of sequence

ACGAGCGCAGCGAGTCAGTGAGCGAGGAAGCCTGCATAACGCGAAGTAATCTTTTCGGTT TTAAAGAAAAAGGGCAGGGTGGTGACACCTTGCCGTTTTTTTGCCGGACTGCAGCGGCCG CTACTAGTATTATTAGCCTTTTAACACTTTTTCGATCAAACTAACATTGCTTAAGAGAAA CGCATCACGAGCACGAGGCGCATCAAAACTAAGGCCGACCGGCAGGCGGCCTTCCGGACC GACCGGAATTGTAATGCTTGGTAACATCGCGTTGCTTGCGAGGTCTGTGTTACGGATGGC CTGGCTAAAAAATTCCGGACGATCTGCATGGTCCAGATGAGGCACGCGACACGGGACCGT CGGATACCCTAAAAGTTTAATGTGATGGCGCGCAAAAAGCTCATCCATCTTGCGTTTCAG CAGCTCTCCATTCTGGAGAGAACTCAGATAATGTGCCGGCTTAATCGCGCCCTCTCCCAG ATGGTGATTAATAATGCCTTTCACATTTGCATCATCCACTTGTGCAAAAATATGATGGAT GCCATGTTTCCAACCTAAGGACAACAAGGATTGAGCAAAATCCGCGAAAAATTCGTACAG CGGCACCGTGAAGGTTAATGTCTGGTTCAATTCCACAATGTCCGCATCGTCGATCTCAAC AAATTCAAAGCCCGTTTTGCGTAAGAGGCTCAGCGCATTGATACAGGCACGTTCTACTTC GCCATCTAAGTCAGACCACATGCTCACCGGCAGGCCGATGCGGCAAGGTCCCGTGCGATT AAGAGGGAAACTCTGCTGTTTTCCTGACAGCAGCCCATACACAAATTCAGCGTCGCCAGC TGTGCGCGTCAGAG

55_premix -- 14..888 of sequence

CTATAAAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCT AAGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGAATTTTGTCAAAATAATTTTATTG ACAACGTCTTATTAACGTTGATATAATTTAAATTTTTTTTGACAAAAATGGGCTCGTGTT GTACAATAAATGTCAGCCTGCGGTCCGGTGGCCTTAACTACCAACTCACCGGAGGGTATA TATGTTTGGACCGGATTTCCCGTTTGCATTTGATGATTGGATCGAACATCCTAAAGGACT TGGGTCAATTCCGGCAGAAAATCACGGCGCTGAGGTTGCAATTATCGGGGCCGGCATTGC AGGCTTAGTAGCAGCATATGAATTAATGAAGATGGGTCTGAAACCTGTAGTCTATGAGGC CTCCAAAATGGGAGGTCGCTTACGGTCACAGGAATTTGAAGGAGCGAAGGGAATTGTTGC GGAACTGGGCGGTATGCGCTTTCCAGTCTCCTCCACAGCGTTCTTTCATTACGTTGATAA ACTGGGCCTTGAAAGTCGTCCGTTCCCTAACCCTTTGACGGCGGCCAGTGGCAGCACGGT GATTGACCTGGAAGGTACCACGTACTATGCACAGATGTTATCAGATCTCCCAGCCCTGTT CCAAGAAGTGGCGGATGCATGGGCAGACGCCTTAGAATCAGGCTCTCAATTTGGAGATAT TCAACAGGCCATTCGTGATTCTGACGTACCGCGTCTCAAGGAACTGTGGAACAAACTCGT GCCTTTGTGGGACGATCGCACTTTCTATGACTTTGTAGCGACCTCAAAGGCCTTTGCGAA ATTAAGCTTTTACCATCGTGAGGTTTCGGACAGTT

Sequence results for our Auxin Xpress module.

56_AUX1 -- 21..841 of sequence

TTCAATAGTATGTTAGAAATCTTTCGTGTTGTGATGACGAATTGTGATGAGCACCAGCAT CTGATTGTTGGTGGCGTACAGCAAGTGCCTGTAGGCCTGTGGTCGCACGTGCCTGAACGC TGCGCACATTGGCCAAAGGGTACGTCTCTTTCCTCACTTCATCGGGGTGCCCCGCGCCCA GGTGTCAAACGTATTGCACGTGCTGAAGATGGTTCATTTACGGTTACAGATAATTGGGGC GATACACGTCAATACGCTGCCGTTTTGACAACCTGTCAATCGTGGCTGCTTACAACGCAG ATTGAATGTGAGGAATCTTTGTTTTCACAGAAGATGTGGATGGCGCTGGATCGCACGCGT TACATGCAGTCTTCTAAAACTTTTGTGATGGTTGATCGGCCGTTTTGGAAGGACAAAGAT CCGGAAACGGGTCGCGATCTTATGTCCATGACCCTCACCGATCGCTTAACGCGCGGCACA TACTTATTTGATAATGGCGACGACAAGCCAGGCGTTATTTGCCTGAGTTACTCATGGATG AGTGACGCTTTAAAAATGTTACCGCAACCGATCGAGAAACGCGTCAAATTAGCTTTAGAT GCCCTTAAAAAGATTTACCCGAAAGTGGATATCGCCGCACGTATTATTGGTGACCCGATT ACTGTGAGTTGGGAAGCGGATCCGCATTTCCTGGGGGCATTTAAAGGCGCGTTACCGGGC CATTACCGTTACAACCAGCGCATGTACGCGCATTTTATGCAGCAGGACATGCCGTCAGAA CAGCGTGGCATGTTTATTGCCGGTGATGATGTATCTTGACA

56_AUX2 -- 16..912 of sequence

GCAGTACAACGTCATTGAACGCGGTGTGGGGCATCATGAACCATTTCGGAGGGAAAACCC ACGCAGAGAATCCGGGTCCTGGCGACGTTTTCCATGAGATTGGACCGATCGCGCTGGGGG ACTAATAATCGCTGGGACGCCCGCCCCTAGAAAAGGAGGACTAAAAATGCGCGAAATGAT TACGCTTGAATCTCTGTGCCGCGCATTGGCAGACGAACAGATTGCCGCAGAAGAATTGCG CGAGCGCGCGCTGGATACAGAGGCTCGCTTAACACTGTTAAATTGCTTCATTCGCGAAGG CGACGCCGTAAGCCAATTCGGTGAAGCGGATCAGGCGCGTAAAGGAACTTCACTTTGGGG GGTGCCTGTGAGTTTTAAGGATAACATCTGTGTTCGCGGGCTGCCACTGACGGCGGGTAC CCGCGGCATGAGCGGCTTTATTGCCGATCAGGATGCAGCAATCGTGAGCCAGTTAAAAGC ATTGGGAGCTGTGGTCGCGGGCAAAAATAACATGCATGAGCTGTCATTTGGCGTCACGAG CATCAATCCACATTGGGGCGCGGTTGGAAACCCGGTGGCTCCAGGATATTGCGCCGGAGG TTCTAGCGGAGGTTCCGCTGCGGCGGTCGCATCTGGAATTGTCCCGCTCAGCGTCGGGAC GGACACAGGCGGCTCAATCCGTATTCCGGCGGCATTTTGCGGCATTACGGGTTTTCGCCC TACTACAGGTCGCTTGAGTACGGCGGGGATCATTCCGGTCAGCCACACGAAGGATTGTGT CGGCCTCCTGACGCGCACAGCTGGCGACGCTGAATTTGTGTATGGGCTGCTGTCAGGAAA ACAGCAGAGTTTCCCTCTTAATCGCACGGGACCTTGCCGCATCGGCCTGCCGGTGAG

56_AUX3 -- 15..922 of sequence

GATGATGTGTCGGCCTCCTGACGCGCACAGCTGGCGACGCTGAATTTGTGTATGGGCTGC TGTCAGGAAAACAGCAGAGTTTCCCTCTTAATCGCACGGGACCTTGCCGCATCGGCCTGC CGGTGAGCATGTGGTCTGACTTAGATGGCGAAGTAGAACGTGCCTGTATCAATGCGCTGA GCCTCTTACGCAAAACGGGCTTTGAATTTGTTGAGATCGACGATGCGGACATTGTGGAAT TGAACCAGACATTAACCTTCACGGTGCCGCTGTACGAATTTTTCGCGGATTTTGCTCAAT CCTTGTTGTCCTTAGGTTGGAAACATGGCATCCATCATATTTTTGCACAAGTGGATGATG CAAATGTGAAAGGCATTATTAATCACCATCTGGGAGAGGGCGCGATTAAGCCGGCACATT ATCTGAGTTCTCTCCAGAATGGAGAGCTGCTGAAACGCAAGATGGATGAGCTTTTTGCGC GCCATCACATTAAACTTTTAGGGTATCCGACGGTCCCGTGTCGCGTGCCTCATCTGGACC ATGCAGATCGTCCGGAATTTTTTAGCCAGGCCATCCGTAACACAGACCTCGCAAGCAACG CGATGTTACCAAGCATTACAATTCCGGTCGGTCCGGAAGGCCGCCTGCCGGTCGGCCTTA GTTTTGATGCGCCTCGTGCTCGTGATGCGTTTCTCTTAAGCAATGTTAGTTTGATCGAAA AAGTGTTAAAAGGCTAATAATACTAGTAGCGGCCGCTGCAGTCCGGCAAAAAAACGGCAA GGTGTCACCACCCTGCCCTTTTTCTTTAAAACCGAAAAGATTACTTCGCGTTATGCACGC TTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCA CTCAACGC

56_premix -- 14..930 of sequence

TATAAAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTA AGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGAATTTTGTCAAAATAATTTTATTGA CAACGTCTTATTAACGTTGATATAATTTAAATTTTTTTTGACAAAAATGGGCTCGTGTTG TACAATAAATGTCAGCCTGCGGTCCGGTGGCCTTAACTACCAACTCACCGGAGGGTATAT ATGTTTGGACCGGATTTCCCGTTTGCATTTGATGATTGGATCGAACATCCTAAAGGACTT GGGTCAATTCCGGCAGAAAATCACGGCGCTGAGGTTGCAATTATCGGGGCCGGCATTGCA GGCTTAGTAGCAGCATATGAATTAATGAAGATGGGTCTGAAACCTGTAGTCTATGAGGCC TCCAAAATGGGAGGTCGCTTACGGTCACAGGAATTTGAAGGAGCGAAGGGAATTGTTGCG GAACTGGGCGGTATGCGCTTTCCAGTCTCCTCCACAGCGTTCTTTCATTACGTTGATAAA CTGGGCCTTGAAAGTCGTCCGTTCCCTAACCCTTTGACGGCGGCCAGTGGCAGCACGGTG ATTGACCTGGAAGGTACCACGTACTATGCACAGATGTTATCAGATCTCCCAGCCCTGTTC CAAGAAGTGGCGGATGCATGGGCAGACGCCTTAGAATCAGGCTCTCAATTTGGAGATATT CAACAGGCCATTCGTGATTCTGACGTACCGCGTCTCAAGGAACTGTGGAACAAACTCGTG CCTTTGTGGGACGATCGCACTTTCTATGACTTTGTAGCGACCTCAAAGGCCTTTGCGAAA TTAAGCTTTTACCATCGTGAGGTTTTCGGACAAGTTGGTTTTGGTACAGGGGGTGGGATT CTGATTTTCCAATAGTA

56_premix -- 15..893 of sequence

GACGAGCGCAGCGAGTCAGTGAGCGAGGAAGCCTGCATAACGCGAAGTAATCTTTTCGGT TTTAAAGAAAAAGGGCAGGGTGGTGACACCTTGCCGTTTTTTTGCCGGACTGCAGCGGCC GCTACTAGTATTATTAGCCTTTTAACACTTTTTCGATCAAACTAACATTGCTTAAGAGAA ACGCATCACGAGCACGAGGCGCATCAAAACTAAGGCCGACCGGCAGGCGGCCTTCCGGAC CGACCGGAATTGTAATGCTTGGTAACATCGCGTTGCTTGCGAGGTCTGTGTTACGGATGG CCTGGCTAAAAAATTCCGGACGATCTGCATGGTCCAGATGAGGCACGCGACACGGGACCG TCGGATACCCTAAAAGTTTAATGTGATGGCGCGCAAAAAGCTCATCCATCTTGCGTTTCA GCAGCTCTCCATTCTGGAGAGAACTCAGATAATGTGCCGGCTTAATCGCGCCCTCTCCCA GATGGTGATTAATAATGCCTTTCACATTTGCATCATCCACTTGTGCAAAAATATGATGGA TGCCATGTTTCCAACCTAAGGACAACAAGGATTGAGCAAAATCCGCGAAAAATTCGTACA GCGGCACCGTGAAGGTTAATGTCTGGTTCAATTCCACAATGTCCGCATCGTCGATCTCAA CAAATTCAAAGCCCGTTTTGCGTAAGAGGCTCAGCGCATTGATACAGGCACGTTCTACTT CGCCATCTAAGTCAGACCACATGCTCACCGGCAGGCCGATGCGGCAAGGTCCCGTGCGAT TAAGAGGGAAACTCTGCTGTTTTCCTGACAGCAGCCCATACACAAATTCAGCGTCGCCAG CTGTGCGCGTCAGGAGGCCGACACAATCCTTCGTGTGGC

Sequence results to determine SpeI site problem in sfGFP

17R_premix -- 35..933 of sequence

AGCGAGTCGGTGAGCGAGGAAGCCTGCATAACGCGAAGTAATCTTTTCGGTTTTAAAGAA AAAGGGCAGGGTGGTGACACCTTGCCCTTTTTTGCCGGACTGCAGCGGCCGCTACTAGGT AGCGAAAAAACCCCGCCGAAGCGGGGTTTTTTGCGCTCTAGTATCATTACTTATACAGCT CGTCCATACCGTGGGTGATGCCCGCAGCGGTCACAAATTCCAACAGGACCATGTGATCAC GTTTTTCGTTCGGATCTTTGCTCAGCACGCTTTGGGTGCTCAGATAGTGATTATCCGGCA GCAAAACCGGACCGTCACCAATTGGGGTGTTCTGCTGGTAATGGTCGGCCAGTTGGACGC TACCGTCCTCAACATTGTGGCGAATCTTGAAATTGGCCTTGATGCCGTTCTTTTGCTTGT CTGCCGTAATGTAAACATTATGGCTGTTGAAGTTATACTCCAGTTTATGGCCCAGAATGT TACCATCCTCTTTAAAGTCGATACCCTTCAATTCGATACGATTGACCAGCGTGTCACCTT CAAACTTAACCTCTGCGCGAGTTTTGTAGTAGCCATCATCCTTGAAGGAAATGGTACGCT CCTGGACGTAACCCTCCGGCATCGCAGATTTGAAGAAATCGTGTTGTTTCATGTGGTCCG GATAACGCGCAAAACACTGCACGCCATACGTCAACGTCGTCACCAAGGTCGGCCAAGGCA CCGGCAGTTTACCGGTGGTGCAGATGAACTTCAGGGTCAGTTTGCCGTTGGTCGCGTCGC CCTCACCTTCACCGCGAACGCTAAACTTATGACCGTTCACATCGCCGTCCAGCTCGACCA GAATCGGAACTACGCCCGTGAACAGTTCTTCGCCTTTACGCATTATCTTACCTCCTTAA

Sequence results for possible PA2652 producing colony

57.20F_premix -- 14..986 of sequence

CTATAAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTA AGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGTTGACGGCTAGCTCAGTCCTAGGTA CAGTGCTAGCCAGCCTGCGGTCCGGTTAAAAAGAAGGGGTTCTAAATGATGCGCTTGACC CTGAAAAGTAAAGTTCTGCTGCTGGCCATGGTACCGGTGCTTTTGTTTGCACTGGTACTG TCAGGCGGCGCCGTACTGATTCTGAAAAAGCAAGCCGATGCCGAAGTCAAAGATACCCGC GAGCGCCTTTTAGGGGATCGCCGCGCGGAACTGGAGCACTACGTGCAAATCGCCATGGGC AGTATTCAAGCAGAATATGATCGCAGCGCGAACGGTGACCTCAACGCCCGCGCCGAAGCC ATTGCGCGCCTGAGCAAGATTAAATATGGAAAAGATGGTTACATTTTCGGATACGATTCT CAGGTCGTGCGGCTGTTTCGCGGGGATTCTCCTGTAGATGTAGGCAAAAGTTTTCGCGAT CGTCGTGATCCGAGCGGAGTGTACTTGAATCGTGAACTTGTCGAAGCCGGACGCAACGGT TCCCACTACGTAACCTATACCTCGCCGCTGCCGGGTAACGAATCGGTGATGGTTCCTAAA CTGTCGTACACGCTGTATCTGCCGAAGTGGGATATGGTAATCGGTAGCGCGATTAATTTG GACGGTGTCGAAGCGCAACTGGTAGAAATTAAACAGGATATTGATGAACGCATCGGCACC CTGATTGCGAGCATTGTGGGTATTGCCGGCGTACTGCTGGTAGTGCTGCTGGTGATTGGG TTAGCAGTGGCGAATGCTATGCTTCGCCCGCTTCATCAGATCCGTCAGAACCTTGATGAT ATTGCCGCCGGCGAGGGTGATCTGACCCGCCGCCTGCCGGTGACCAGCTACGATGAACTC GGTGAACTGGCAG

57.20R_premix -- 13..1023 of sequence

ACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCCTGCATAACGCGAAGTAATCTTTTC GGTTTTAAAGAAAAAGGGCAGGGTGGTGACACCTTGCCCTTTTTTGCCGGACTGCAGCGG CCGCTACTAGTATTATTAGATGCGAAACTGGCCCACCAGACGACCTAAGCCTTGACCCAG CTGCGCCAGTGACCGAGCCGTTTGTGCACCTTGCTGTGCTTCATCCGCCACTGAATCAAC CGCGCCCGCGATCTGGTGTACAGAGCGATTAATCTCTTCTGCCACTGCGGTTTGTTCTTC TGCCGCGCTCGCGATCTGCGCATTCATCGCATTAATGGTGGCAATCAGCTGGGCGATCGC ATCCAGGGAATCGCCTGCCTGATTCGCCTGATTTGAGGTTCCCTCTCCCGCTTCACCGCT GCGCCGCATGGCATCAACGGCGGCATTGGTGCCCTGTTGCAGACGATCGATCATACCCTG GATTTCCTGGGTGCTCTGCTGGGTGCGAGAAGCCAGTGCGCGCACCTCATCCGCGACGAC GGCAAATCCACGACCTGCTTCGCCTGCACGTGCTGCCTCAATGGCCGCATTCAGGGCCAG CAAATTAGTTTGTTCCGCAATGGAACGAATCACGCCCAGCACCGAGACAATGCTCTGGAC ATCTTGCTGAAGGCTATCGAGACTGGTGCCCGAATCGCGGATTTCGTCTACCAGGGCATG AATACGCTCAATAGAACCATCCACTACACGCTTAGCTGCCTGGCCTTCATGATCTGTTTG CTGAGCTGCCTCGGCTGCGCGTTGAGCGCTTTGTGCCACTTCGTGTGCGGCGGCGCTCAT CTCGTTAATCGCGGTGGCAACCTGATCGGTCTCGTGACGCTGACGTTCCATCGCTTGTTC AGAACGTTCTGCCTGCGCGCTCATTTGCTCCACTAACTGTTTCAGATCGCCCGTCATCCC TGCGATCTGGCGAACTAAGCCATGAATTTTTCAACGAAACGGTTAAAGGAG

Sequence results for possible PA2652 producing colony

57.3F_premix -- 15..985 of sequence

TATAAAAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCT AAGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGTTGACGGCTAGCTCAGTCCTAGGT ACAGTGCTAGCCAGCCTGCGGTCCGGTTAAAAAGAAGGGGTTCTAAATGATGCGCTTGAC CCTGAAAAGTAAAGTTCTGCTGCTGGCCATGGTACCGGTGCTTTTGTTTGCACTGGTACT GTCAGGCGGCGCCGTACTGATTCTGAAAAAGCAAGCCGATGCCGAAGTCAAAGATACCCG CGAGCGCCTTTTAGGGGATCGCCGCGCGGAACTGGAGCACTACGTGCAAATCGCCATGGG CAGTATTCAAGCAGAATATGATCGCAGCGCGAACGGTGACCTCAACGCCCGCGCCGAAGC CATTGCGCGCCTGAGCAAGATTAAATATGGAAAAGATGGTTACATTTTCGGATACGATTC TCAGGTCGTGCGGCTGTTTCGCGGGGATTCTCCTGTAGATGTAGGCAAAAGTTTTCGCGA TCGTCGTGATCCGAGCGGAGTGTACTTGAATCGTGAACTTGTCGAAGCCGGACGCAACGG TTCCCACTACGTAACCTATACCTCGCCGCTGCCGGGTAACGAATCGGTGATGGTTCCTAA ACTGTCGTACACGCTGTATCTGCCGAAGTGGGATATGGTAATCGGTAGCGCGATTAATTT GGACGGTGTCGAAGCGCAACTGGTAGAAATTAAACAGGATATTGATGAACGCATCGGCAC CCTGATTGCGAGCATTGTGGGTATTGCCGGCGTACTGCTGGTAGTGCTGCTGGTGATTGG GTTAGCAGTGGCGAATGCTATGCTTCGCCCGCTTCATCAGATCCGTCAGAACCCTTGATG ATATTGCCGCCGGCGAGGGTGATCTGACCCGCCGCCTGCCGGTGACCAGCTACGATGAAC TCGGTGAACTG

57.3R_premix -- 15..1019 of sequence

ACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCCTGCATAACGCGAAGTAATCTTTTC GGTTTTAAAGAAAAAGGGCAGGGTGGTGACACCTTGCCCTTTTTTGCCGGACTGCAGCGG CCGCTACTAGTATTATTAGATGCGAAACTGGCCCACCAGACGACCTAAGCCTTGACCCAG CTGCGCCAGTGACCGAGCCGTTTGTGCACCTTGCTGTGCTTCATCCGCCACTGAATCAAC CGCGCCCGCGATCTGGTGTACAGAGCGATTAATCTCTTCTGCCACTGCGGTTTGTTCTTC TGCCGCGCTCGCGATCTGCGCATTCATCGCATTAATGGTGGCAATCAGCTGGGCGATCGC ATCCAGGGAATCGCCTGCCTGATTCGCCTGATTTGAGGTTCCCTCTCCCGCTTCACCGCT GCGCCGCATGGCATCAACGGCGGCATTGGTGCCCTGTTGCAGACGATCGATCATACCCTG GATTTCCTGGGTGCTCTGCTGGGTGCGAGAAGCCAGTGCGCGCACCTCATCCGCGACGAC GGCAAATCCACGACCTGCTTCGCCTGCACGTGCTGCCTCAATGGCCGCATTCAGGGCCAG CAAATTAGTTTGTTCCGCAATGGAACGAATCACGCCCAGCACCGAGACAATGCTCTGGAC ATCTTGCTGAAGGCTATCGAGACTGGTGCCCGAATCGCGGATTTCGTCTACCAGGGCATG AATACGCTCAATAGAACCATCCACTACACGCTTAGCTGCCTGGCCTTCATGATCTGTTTG CTGAGCTGCCTCGGCTGCGCGTTGAGCGCTTTGTGCCACTTCGTGTGCGGCGGCGCTCAT CTCGTTAATCGCGGTGGCAACCTGATCGGTCTCGTGACGCTGACGTTCCATCGCTTGTTC AGAACGTTCTGCCTGCGCGCTCATTTGCTCCACTAACTGTTTCAGATCGCCCGTCATCCC TGCGATCTGGCGAACTAAGCCATGAATTTTTTCAACGAAACGGTT

Sequence results for possible PA2652 producing colony

57.7F_premix -- 14..987 of sequence

TATAAAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTA AGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGTTGACGGCTAGCTCAGTCCTAGGTA CAGTGCTAGCCAGCCTGCGGTCCGGTTAAAAAGAAGGGGTTCTAAATGATGCGCTTGACC CTGAAAAGTAAAGTTCTGCTGCTGGCCATGGTACCGGTGCTTTTGTTTGCACTGGTACTG TCAGGCGGCGCCGTACTGATTCTGAAAAAGCAAGCCGATGCCGAAGTCAAAGATACCCGC GAGCGCCTTTTAGGGGATCGCCGCGCGGAACTGGAGCACTACGTGCAAATCGCCATGGGC AGTATTCAAGCAGAATATGATCGCAGCGCGAACGGTGACCTCAACGCCCGCGCCGAAGCC ATTGCGCGCCTGAGCAAGATTAAATATGGAAAAGATGGTTACATTTTCGGATACGATTCT CAGGTCGTGCGGCTGTTTCGCGGGGATTCTCCTGTAGATGTAGGCAAAAGTTTTCGCGAT CGTCGTGATCCGAGCGGAGTGTACTTGAATCGTGAACTTGTCGAAGCCGGACGCAACGGT TCCCACTACGTAACCTATACCTCGCCGCTGCCGGGTAACGAATCGGTGATGGTTCCTAAA CTGTCGTACACGCTGTATCTGCCGAAGTGGGATATGGTAATCGGTAGCGCGATTAATTTG GACGGTGTCGAAGCGCAACTGGTAGAAATTAAACAGGATATTGATGAACGCATCGGCACC CTGATTGCGAGCATTGTGGGTATTGCCGGCGTACTGCTGGTAGTGCTGCTGGTGATTGGG TTAGCAGTGGCGAATGCTATGCTTCGCCCGCTTCATCAGATCCGTCAGAACCTTGATGAT ATTGCCGCCGGCGAGGGTGATCTGACCCGCCGCCTGCCGGTGACCAGCTACGATGAACTC GGTGAACTGGCAGC

57.7R_premix -- 15..1032 of sequence

GACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCCTGCATAACGCGAAGTAATCTTTT CGGTTTTAAAGAAAAAGGGCAGGGTGGTGACACCTTGCCCTTTTTTGCCGGACTGCAGCG GCCGCTACTAGTATTATTAGATGCGAAACTGGCCCACCAGACGACCTAAGCCTTGACCCA GCTGCGCCAGTGACCGAGCCGTTTGTGCACCTTGCTGTGCTTCATCCGCCACTGAATCAA CCGCGCCCGCGATCTGGTGTACAGAGCGATTAATCTCTTCTGCCACTGCGGTTTGTTCTT CTGCCGCGCTCGCGATCTGCGCATTCATCGCATTAATGGTGGCAATCAGCTGGGCGATCG CATCCAGGGAATCGCCTGCCTGATTCGCCTGATTTGAGGTTCCCTCTCCCGCTTCACCGC TGCGCCGCATGGCATCAACGGCGGCATTGGTGCCCTGTTGCAGACGATCGATCATACCCT GGATTTCCTGGGTGCTCTGCTGGGTGCGAGAAGCCAGTGCGCGCACCTCATCCGCGACGA CGGCAAATCCACGACCTGCTTCGCCTGCACGTGCTGCCTCAATGGCCGCATTCAGGGCCA GCAAATTAGTTTGTTCCGCAATGGAACGAATCACGCCCAGCACCGAGACAATGCTCTGGA CATCTTGCTGAAGGCTATCGAGACTGGTGCCCGAATCGCGGATTTCGTCTACCAGGGCAT GAATACGCTCAATAGAACCATCCACTACACGCTTAGCTGCCTGGCCTTCATGATCTGTTT GCTGAGCTGCCTCGGCTGCGCGTTGAGCGCTTTGTGCCACTTCGTGTGCGGCGGCGCTCA TCTCGTTAATCGCGGTGGCAACCTGATCGGTCTCGTGACGCTGACGTTCCATCGCTTGTT CAGAACGTTCTGCCTGCGCGCTCATTTGCTCCACTAACTGTTTCAGATCGCCCGTCATCC CTGCGATCTGGCGAACTAAGCCATGAATTTTTTCAACGAAACGGTTAAAGGAGCCTGC

Sequence results for our PA2652 producing construct. Correct.

57.8F_premix -- 15..979 of sequence

TATAAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTAA GGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGTTGACGGCTAGCTCAGTCCTAGGTAC AGTGCTAGCCAGCCTGCGGTCCGGTTAAAAAGAAGGGGTTCTAAATGATGCGCTTGACCC TGAAAAGTAAAGTTCTGCTGCTGGCCATGGTACCGGTGCTTTTGTTTGCACTGGTACTGT CAGGCGGCGCCGTACTGATTCTGAAAAAGCAAGCCGATGCCGAAGTCAAAGATACCCGCG AGCGCCTTTTAGGGGATCGCCGCGCGGAACTGGAGCACTACGTGCAAATCGCCATGGGCA GTATTCAAGCAGAATATGATCGCAGCGCGAACGGTGACCTCAACGCCCGCGCCGAAGCCA TTGCGCGCCTGAGCAAGATTAAATATGGAAAAGATGGTTACATTTTCGGATACGATTCTC AGGTCGTGCGGCTGTTTCGCGGGGATTCTCCTGTAGATGTAGGCAAAAGTTTTCGCGATC GTCGTGATCCGAGCGGAGTGTACTTGAATCGTGAACTTGTCGAAGCCGGACGCAACGGTT CCCACTACGTAACCTATACCTCGCCGCTGCCGGGTAACGAATCGGTGATGGTTCCTAAAC TGTCGTACACGCTGTATCTGCCGAAGTGGGATATGGTAATCGGTAGCGCGATTAATTTGG ACGGTGTCGAAGCGCAACTGGTAGAAATTAAACAGGATATTGATGAACGCATCGGCACCC TGATTGCGAGCATTGTGGGTATTGCCGGCGTACTGCTGGTAGTGCTGCTGGTGATTGGGT TAGCAGTGGCGAATGCTATGCTTCGCCCGCTTCATCAGATCCGTCAGAACCCTTGATGAT ATTGCCGCCGGCGAGGGTGATCTGACCCGCCGCCTGCCGGTGACCAGCTACGATGACTCG GTGAC

57.8R_premix -- 14..1022 of sequence

ACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCCTGCATAACGCGAAGTAATCTTTTC GGTTTTAAAGAAAAAGGGCAGGGTGGTGACACCTTGCCCTTTTTTGCCGGACTGCAGCGG CCGCTACTAGTATTATTAGATGCGAAACTGGCCCACCAGACGACCTAAGCCTTGACCCAG CTGCGCCAGTGACCGAGCCGTTTGTGCACCTTGCTGTGCTTCATCCGCCACTGAATCAAC CGCGCCCGCGATCTGGTGTACAGAGCGATTAATCTCTTCTGCCACTGCGGTTTGTTCTTC TGCCGCGCTCGCGATCTGCGCATTCATCGCATTAATGGTGGCAATCAGCTGGGCGATCGC ATCCAGGGAATCGCCTGCCTGATTCGCCTGATTTGAGGTTCCCTCTCCCGCTTCACCGCT GCGCCGCATGGCATCAACGGCGGCATTGGTGCCCTGTTGCAGACGATCGATCATACCCTG GATTTCCTGGGTGCTCTGCTGGGTGCGAGAAGCCAGTGCGCGCACCTCATCCGCGACGAC GGCAAATCCACGACCTGCTTCGCCTGCACGTGCTGCCTCAATGGCCGCATTCAGGGCCAG CAAATTAGTTTGTTCCGCAATGGAACGAATCACGCCCAGCACCGAGACAATGCTCTGGAC ATCTTGCTGAAGGCTATCGAGACTGGTGCCCGAATCGCGGATTTCGTCTACCAGGGCATG AATACGCTCAATAGAACCATCCACTACACGCTTAGCTGCCTGGCCTTCATGATCTGTTTG CTGAGCTGCCTCGGCTGCGCGTTGAGCGCTTTGTGCCACTTCGTGTGCGGCGGCGCTCAT CTCGTTAATCGCGGTGGCAACCTGATCGGTCTCGTGACGCTGACGTTCCATCGCTTGTTC AGAACGTTCTGCCTGCGCGCTCATTTGCTCCACTAACTGTTTCAGATCGCCCGTCATCCC TGCGATCTGGCGAACTAAGCCATGAATTTTTCAACGAAACGGTTAAAGG

Dendra2 forward sequencing results after ligation with Ptet and RBS

Dendra_forward_premix -- 15..179 of sequence

TATAAAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTA AGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGTCCCTATCAGTGATAGAGATTGACA TCCCTATCAGTGATAGAGATACTGAGCACTACTAGATGAACATCA

Results of J23103 ligation into RFP. Correct but Kirsten says suffix is strange

21+60a_frwrd_premix -- 15..921 of sequence

ATAATAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTA AGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGCTGATAGCTAGCTCAGTCCTAGGGA TTATGCTAGCTACTAGAGAAAGAGGAGAAATACTAGATGGCTTCCTCCGAAGACGTTATC AAAGAGTTCATGCGTTTCAAAGTTCGTATGGAAGGTTCCGTTAACGGTCACGAGTTCGAA ATCGAAGGTGAAGGTGAAGGTCGTCCGTACGAAGGTACCCAGACCGCTAAACTGAAAGTT ACCAAAGGTGGTCCGCTGCCGTTCGCTTGGGACATCCTGTCCCCGCAGTTCCAGTACGGT TCCAAAGCTTACGTTAAACACCCGGCTGACATCCCGGACTACCTGAAACTGTCCTTCCCG GAAGGTTTCAAATGGGAACGTGTTATGAACTTCGAAGACGGTGGTGTTGTTACCGTTACC CAGGACTCCTCCCTGCAAGACGGTGAGTTCATCTACAAAGTTAAACTGCGTGGTACCAAC TTCCCGTCCGACGGTCCGGTTATGCAGAAAAAAACCATGGGTTGGGAAGCTTCCACCGAA CGTATGTACCCGGAAGACGGTGCTCTGAAAGGTGAAATCAAAATGCGTCTGAAACTGAAA GACGGTGGTCACTACGACGCTGAAGTTAAAACCACCTACATGGCTAAAAAACCGGTTCAG CTGCCGGGTGCTTACAAAACCGACATCAAACTGGACATCACCTCCCACAACGAAGACTAC ACCATCGTTGAACAGTACGAACGTGCTGAAGGTCGTCACTCCACCGGTGCTTAATAACGC TGATAGTGCTAGTGTAGATCGCTACTAGAGATTAAAGAGGAGAAATACTAGTAGCGGCCG CTGCAGG

Results of J23103 ligation into RFP. Correct but Kirsten says suffix is strange

21+60b_frwrd_premix -- 18..928 of sequence

TATATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTAAGG ATGATTTCTGGAATTCGCGGCCGCTTCTAGAGCTGATAGCTAGCTCAGTCCTAGGGATTA TGCTAGCTACTAGAGAAAGAGGAGAAATACTAGATGGCTTCCTCCGAAGACGTTATCAAA GAGTTCATGCGTTTCAAAGTTCGTATGGAAGGTTCCGTTAACGGTCACGAGTTCGAAATC GAAGGTGAAGGTGAAGGTCGTCCGTACGAAGGTACCCAGACCGCTAAACTGAAAGTTACC AAAGGTGGTCCGCTGCCGTTCGCTTGGGACATCCTGTCCCCGCAGTTCCAGTACGGTTCC AAAGCTTACGTTAAACACCCGGCTGACATCCCGGACTACCTGAAACTGTCCTTCCCGGAA GGTTTCAAATGGGAACGTGTTATGAACTTCGAAGACGGTGGTGTTGTTACCGTTACCCAG GACTCCTCCCTGCAAGACGGTGAGTTCATCTACAAAGTTAAACTGCGTGGTACCAACTTC CCGTCCGACGGTCCGGTTATGCAGAAAAAAACCATGGGTTGGGAAGCTTCCACCGAACGT ATGTACCCGGAAGACGGTGCTCTGAAAGGTGAAATCAAAATGCGTCTGAAACTGAAAGAC GGTGGTCACTACGACGCTGAAGTTAAAACCACCTACATGGCTAAAAAACCGGTTCAGCTG CCGGGTGCTTACAAAACCGACATCAAACTGGACATCACCTCCCACAACGAAGACTACACC ATCGTTGAACAGTACGAACGTGCTGAAAGGTCGTCACTCCACCGGTGCTTAATAACGCTG ATAGTGCTAGTGTAGATCGCTACTAGAGATTAAAGAGGAGAAATACTAGTAGCGGCCGCT GCAGGCTTCCT

Results of J23103 ligation into RFP. Correct but Kirsten says suffix is strange

21+60c_frwrd_premix -- 18..926 of sequence

AAGATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTAAGG ATGATTTCTGGAATTCGCGGCCGCTTCTAGAGCTGATAGCTAGCTCAGTCCTAGGGATTA TGCTAGCTACTAGAGAAAGAGGAGAAATACTAGATGGCTTCCTCCGAAGACGTTATCAAA GAGTTCATGCGTTTCAAAGTTCGTATGGAAGGTTCCGTTAACGGTCACGAGTTCGAAATC GAAGGTGAAGGTGAAGGTCGTCCGTACGAAGGTACCCAGACCGCTAAACTGAAAGTTACC AAAGGTGGTCCGCTGCCGTTCGCTTGGGACATCCTGTCCCCGCAGTTCCAGTACGGTTCC AAAGCTTACGTTAAACACCCGGCTGACATCCCGGACTACCTGAAACTGTCCTTCCCGGAA GGTTTCAAATGGGAACGTGTTATGAACTTCGAAGACGGTGGTGTTGTTACCGTTACCCAG GACTCCTCCCTGCAAGACGGTGAGTTCATCTACAAAGTTAAACTGCGTGGTACCAACTTC CCGTCCGACGGTCCGGTTATGCAGAAAAAAACCATGGGTTGGGAAGCTTCCACCGAACGT ATGTACCCGGAAGACGGTGCTCTGAAAGGTGAAATCAAAATGCGTCTGAAACTGAAAGAC GGTGGTCACTACGACGCTGAAGTTAAAACCACCTACATGGCTAAAAAACCGGTTCAGCTG CCGGGTGCTTACAAAACCGACATCAAACTGGACATCACCTCCCACAACGAAGACTACACC ATCGTTGAACAGTACGAACGTGCTGAAGTCGTCACTCCACCGGTGCTTAATAACGCTGAT AGTGCTAGTGTAGATCGCTACTAGAGATTAAAGAGGAGAAATACTAGTAGCGGCCGCTGC AGGCTTCCT

Results of corrected SpeI site of sfGFP

sfGFP_reverse_premix -- 20..288 of sequence

ACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCCTGCATAACGCGAAGTAATCTTTTCGGT TTTAAAGAAAAAGGGCAGGGTGGTGACACCTTGCCCTTTTTTGCCGGACTGCAGCGGCCG CTACTAGTATCATTACTTATACAGCTCGTCCATACCGTGGGTGATGCCCGCAGCGGTCAC AAATTCCAGCAGGACCATGTGATCACGTTTTTCGTTCGGATCTTTGCTCAGCACGCTTTG GGTGCTCAGATAGTGATTATGCCGGAGCC

Sequence of Anti-holin colony 2

70.2 F_premix -- 25..943 of sequence

AAAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTAAGG ATGATTTCTGGAATTCGCGGCCGCTTCTAGAGTTGACGGCTAGCTCAGTCCTAGGTACAG TGCTAGCCAGCCTGCGGTCCGGGCGATAATAAGGAGGTACTAATGGCCTTAAAAGCAACA GCACTTTTTGCCATGCTAGGATTGTCATTTGTTTTATCTCCATCGATTGAAGCGAATGTC GATCCTCATTTTGATAAATTTATGGAATCTGGTATTAGGCACGTTTATATGCTTTTTGAA AATAAAAGCGTAGAATCGTCTGAACAATTCTATAGTTTTATGAGAACGACCTATAAAAAT GACCCGTGCTCTTCTGATTTTGAATGTATAGAGCGAGGCGCGGAGATGGCACAATCATAC GCTAGAATTATGAACATTAAATTGGAGACTGAATAATAACCAGGCATCAAATAAAACGAA AGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCT ACTAGAGTCACACTGGCTCACCTTCGGGTGGGCCTTTCTGCGTTTATATACTAGTAGCGG CCGCTGCAGTCCGGCAAAAAAGGGCAAGGTGTCACCACCCTGCCCTTTTTCTTTAAAACC GAAAAGATTACTTCGCGTTATGCAGGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGT TCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATC AGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAA AAAGGCCGCGTTGCTGGCGTTTTTCCACAGGCTCCGCCCCCCTGACGAGCATCACAAAAA TCGACGCTCAAGTCAGAGT

70.2 R_premix -- 19..1016 of sequence

GACGAGCGCAGCGAGTCAGTGAGCGAGGAAGCCTGCATAACGCGAAGTAATCTTTTCGGT TTTAAAGAAAAAGGGCAGGGTGGTGACACCTTGCCCTTTTTTGCCGGACTGCAGCGGCCG CTACTAGTATATAAACGCAGAAAGGCCCACCCGAAGGTGAGCCAGTGTGACTCTAGTAGA GAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTC GTTTTATTTGATGCCTGGTTATTATTCAGTCTCCAATTTAATGTTCATAATTCTAGCGTA TGATTGTGCCATCTCCGCGCCTCGCTCTATACATTCAAAATCAGAAGAGCACGGGTCATT TTTATAGGTCGTTCTCATAAAACTATAGAATTGTTCAGACGATTCTACGCTTTTATTTTC AAAAAGCATATAAACGTGCCTAATACCAGATTCCATAAATTTATCAAAATGAGGATCGAC ATTCGCTTCAATCGATGGAGATAAAACAAATGACAATCCTAGCATGGCAAAAAGTGCTGT TGCTTTTAAGGCCATTAGTACCTCCTTATTATCGCCCGGACCGCAGGCTGGCTAGCACTG TACCTAGGACTGAGCTAGCCGTCAACTCTAGAAGCGGCCGCGAATTCCAGAAATCATCCT TAGCGAAAGCTAAGGATTTTTTTTATCTGAAATTCTGCCTCGTGATACGCCTATTTTTAT AGGTTAATGTCATGATAATAATGGTTTCTTAGACGTCAGGTGGCACTCGAGTTGATCGGG CACGTAAGAGGTTCCAACTTTCACCATAATGAAATAAGATCACTACCGGGCGTATTTTTT GAGTTATCGAGATTTTCAGGAGCTAAGGAAGCTAAAATGGAGAAAAAAATCACTGGATAT ACCACCGTTGATATATCCCAATGGCATCGTAAAGAACATTTTGAGGCATTTCAGTCAGTT GCTCAATGTACCTATAACCAGACCGTTCAGCTGGATAT

Sequence of Anti-holin colony 2

70.9 F_premix -- 18..1008 of sequence

ATAAAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTAA GGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGTTGACGGCTAGCTCAGTCCTAGGTAC AGTGCTAGCCAGCCTGCGGTCCGGGCGATAATAAGGAGGTACTAATGGCCTTAAAAACAA CAGCACTTTGGCACAATCATACGCTAGAATTATGAACATTAAATTGGAGACTGAATAATA ACCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGT TGTTTGTCGGTGAACGCTCTCTACTAGAGTCACACTGGCTCACCTTCGGGTGGGCCTTTC TGCGTTTATATACTAGTAGCGGCCGCTGCAGTCCGGCAAAAAAGGGCAAGGTGTCACCAC CCTGCCCTTTTTCTTTAAAACCGAAAAGATTACTTCGCGTTATGCAGGCTTCCTCGCTCA CTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGG TAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCC AGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCACAGGCTCCGCC CCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGAC TATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCC TGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATA GCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGC ACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCA ACCCGGTAAGACACGACTTATCGCCACTGGC

70.9 R_premix -- 21..998 of sequence

ACGAGCGCAGCGAGTCAGTGAGCGAGGAAGCCTGCATAACGCGAAGTAATCTTTTCGGTT TTAAAGAAAAAGGGCAGGGTGGTGACACCTTGCCCTTTTTTGCCGGACTGCAGCGGCCGC TACTAGTATATAAACGCAGAAAGGCCCACCCGAAGGTGAGCCAGTGTGACTCTAGTAGAG AGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCG TTTTATTTGATGCCTGGTTATTATTCAGTCTCCAATTTAATGTTCATAATTCTAGCGTAT GATTGTGCCAAAGTGCTGTTGTTTTTAAGGCCATTAGTACCTCCTTATTATCGCCCGGAC CGCAGGCTGGCTAGCACTGTACCTAGGACTGAGCTAGCCGTCAACTCTAGAAGCGGCCGC GAATTCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATTCTGCCTC GTGATACGCCTATTTTTATAGGTTAATGTCATGATAATAATGGTTTCTTAGACGTCAGGT GGCACTCGAGTTGATCGGGCACGTAAGAGGTTCCAACTTTCACCATAATGAAATAAGATC ACTACCGGGCGTATTTTTTGAGTTATCGAGATTTTCAGGAGCTAAGGAAGCTAAAATGGA GAAAAAAATCACTGGATATACCACCGTTGATATATCCCAATGGCATCGTAAAGAACATTT TGAGGCATTTCAGTCAGTTGCTCAATGTACCTATAACCAGACCGTTCAGCTGGATATTAC GGCCTTTTTAAAGACCGTAAAGAAAAATAAGCACAAGTTTTATCCGGCCTTTATTCACAT TCTTGCCCGCCTGATGAATGCTCATCCGGAATTTCGTATGGCAATGAAAGACGGTGAGCT GGTGATATGGGATAGTGTTCACCCTTGTTACACCGTTTTCCATGAGCAAACTGAAACGTT TTCATCGCTCTGGAGTGA

Sequence of Dendra2 construct. Verified.

71.2F_premix -- 14..961 of sequence ATAAAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTAA GGATGATTTCTGGAATTCGCGGCCGCTTCTAGATGAACCTCATCAAAGAGGATATGCGCG TAAAAGTCCACATGGAAGGCAACGTGAATGGTCATGCGTTCGTAATCGAAGGTGAAGGCA AAGGGAAACCGTACGAAGGTACGCAAACTGCAAACTTGACCGTGAAAGAAGGTGCACCTC TGCCATTCAGCTACGACATTCTTACCACTGCGGTTCACTATGGCAATCGTGTCTTTACGA AATATCCGGAAGATATTCCGGACTACTTCAAACAGTCGTTTCCGGAAGGCTATTCATGGG AGCGTACCATGACCTTTGAGGATAAAGGCATTTGCACAATTCGCAGCGATATTAGCCTGG AAGGGGACTGCTTCTTTCAGAACGTTCGGTTTAAAGGCACGAATTTTCCTCCGAATGGGC CGGTTATGCAGAAGAAAACCCTGAAATGGGAACCGTCGACAGAGAAACTGCATGTGCGTG ATGGACTTCTGGTCGGTAACATCAACATGGCCTTACTGTTGGAAGGTGGAGGCCATTATC TCTGTGACTTCAAAACCACGTATAAGGCGAAGAAAGTGGTACAGCTGCCAGATGCCCACT TTGTGGATCATCGCATCGAAATTCTGGGCAATGACTCCGATTACAACAAAGTCAAGCTGT ACGAGCATGCTGTTGCTCGCTATAGTCCCTTACCCTCTCAAGTGTGGTGATAATACTAGT AGCGGCCGCTGCAGTCCGGCAAAAAAACGGCAAGGTGTCACCACCCTGCCCTTTTTCTTT AAAACCGAAAAGATTACTTCGCGTTATGCAGGCTTCCTCGCTCACTGACTCGCTGCGCTC GGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAAT

71.2R_premix -- 16..995 of sequence GACGAGCGCAGCGAGTCAGTGAGCGAGGAAGCCTGCATAACGCGAAGTAATCTTTTCGGT TTTAAAGAAAAAGGGCAGGGTGGTGACACCTTGCCGTTTTTTTGCCGGACTGCAGCGGCC GCTACTAGTATTATCACCACACTTGAGAGGGTAAGGGACTATAGCGAGCAACAGCATGCT CGTACAGCTTGACTTTGTTGTAATCGGAGTCATTGCCCAGAATTTCGATGCGATGATCCA CAAAGTGGGCATCTGGCAGCTGTACCACTTTCTTCGCCTTATACGTGGTTTTGAAGTCAC AGAGATAATGGCCTCCACCTTCCAACAGTAAGGCCATGTTGATGTTACCGACCAGAAGTC CATCACGCACATGCAGTTTCTCTGTCGACGGTTCCCATTTCAGGGTTTTCTTCTGCATAA CCGGCCCATTCGGAGGAAAATTCGTGCCTTTAAACCGAACGTTCTGAAAGAAGCAGTCCC CTTCCAGGCTAATATCGCTGCGAATTGTGCAAATGCCTTTATCCTCAAAGGTCATGGTAC GCTCCCATGAATAGCCTTCCGGAAACGACTGTTTGAAGTAGTCCGGAATATCTTCCGGAT ATTTCGTAAAGACACGATTGCCATAGTGAACCGCAGTGGTAAGAATGTCGTAGCTGAATG GCAGAGGTGCACCTTCTTTCACGGTCAAGTTTGCAGTTTGCGTACCTTCGTACGGTTTCC CTTTGCCTTCACCTTCGATTACGAACGCATGACCATTCACGTTGCCTTCCATGTGGACTT TTACGCGCATATCCTCTTTGATGAGGTTCATCTAGAAGCGGCCGCGAATTCCAGAAATCA TCCTTAGCGAAAGCTAAGGATTTTTTTTTATCTGAAATTCTGCCTCGTGATACGCCTATT TTTATAGGTTAATGTCATGATAATAATGGTTTCTTAGACGTCAGTGGCACTCGAGTTGAT CGGGCACGTAAGAGGTTCCA

Ptet-Dendra2 composite sequencing results. Sequencing results were bad probably due to low concentration of DNA being sent off. One possible base pair mutation and 7% of sequence was not covered.

72F_premix -- 15..135 of sequence TAAAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTAAG GATGATTTCTGGAATTCGCGGCCGCTTCTAGAGTCCCTATCAGTGATAGAGATACTGATC C

72R_premix -- 20..851 of sequence ACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCCTGCATAACGCGAAGTAATCTTTTCGGT TTTAAAGAAAAAGGGCAGGGTGGTGACACCTTGCCGTTTTTTTGCCGGACTGCAGCGGCC GCTACTAGTATTATCACCACACTTGAGAGGGTAAGGGACTATAGCGAGCAACAGCATGCT CGTACAGCTTGACTTTGTTGTAATCGGAGTCATTGCCCAGAATTTCGATGCGATGATCCA CAAAGTGGGCATCTGGCAGCTGTACCACTTTCTTCGCCTTATACGTGGTTTTGAAGTCAC AGAGATAATGGCCTCCACCTTCCAACAGTAAGGCCATGTTGATGTTACCGACCAGAAGTC CATCACGCACATGCAGTTTCTCTGTCGACGGTTCCCATTTCAGGGTTTTCTTCTGCATAA CCGGCCCATTCGGAGGAAAATTCGTGCCTTTAAACCGAACGTTCTGAAAGAAGCAGTCCC CTTCCAGGCTAATATCGCTGCGAATTGTGCAAATGCCTTTATCCTCAAAGGTCATGGTAC GCTCCCATGAATAGCCTTCCGGAAACGACTGTTTGAAGTAGTCCGGAATATCTTCCGGAT ATTTCGTAAAGACACGATTGCCATAGTGAACCGCAGTGGTAAGAATGTCGTAGCTGAATG GCAGAGGTGCACCTTCTTTCACGGTCAAGTTTGCAGTTTGCGTACCTTCGTACGGTTTCC CTTTGCCTTCACCTTCGATTACGAACGCATGACCATTCACGTTGCCTTCCATGTGGACTT TTACGCGCATATCCTCTTTGATGAGGTTCATCTAGTATTGCTCAGCATCTCT

J23100-sfGFP composite. Sequence verified.

73F_premix -- 22..1003 of sequence AAAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTAAGG ATGATTTCTGGAATTCGCGGCCGCTTCTAGAGTTGACGGCTAGCTCAGTCCTAGGTACAG TGCTAGCTACTAGAGAAATCAAATTAAGGAGGTAAGATAATGCGTAAAGGCGAAGAACTG TTCACGGGCGTAGTTCCGATTCTGGTCGAGCTGGACGGCGATGTGAACGGTCATAAGTTT AGCGTTCGCGGTGAAGGTGAGGGCGACGCGACCAACGGCAAACTGACCCTGAAGTTCATC TGCACCACCGGTAAACTGCCGGTGCCTTGGCCGACCTTGGTGACGACGTTGACGTATGGC GTGCAGTGTTTTGCGCGTTATCCGGACCACATGAAACAACACGATTTCTTCAAATCTGCG ATGCCGGAGGGTTACGTCCAGGAGCGTACCATTTCCTTCAAGGATGATGGCTACTACAAA ACTCGCGCAGAGGTTAAGTTTGAAGGTGACACGCTGGTCAATCGTATCGAATTGAAGGGT ATCGACTTTAAAGAGGATGGTAACATTCTGGGCCATAAACTGGAGTATAACTTCAACAGC CATAATGTTTACATTACGGCAGACAAGCAAAAGAACGGCATCAAGGCCAATTTCAAGATT CGCCACAATGTTGAGGACGGTAGCGTCCAACTGGCCGACCATTACCAGCAGAACACCCCA ATTGGTGACGGTCCGGTTTTGCTGCCGGATAATCACTATCTGAGCACCCAAAGCGTGCTG AGCAAAGATCCGAACGAAAAACGTGATCACATGGTCCTGCTGGAATTTGTGACCGCTGCG GGCATCACCCACGGTATGGACGAGCTGTATAAGTAATGATACTAGTAGCGGCCGCTGCAG TCCGGCAAAAAAGGGCAAGGTGTCACCACCCTGCCCTTTTTCTTTAAAACCGAAAAGATT ACTTCGCGTTATGCAGGCTTCC

73R_premix -- 19..338 of sequence GACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCCTGCATAACGCGAAGTAATCTTTTCGG TTTTAAAGAAAAAGGGCAGGGTGGTGACACCTTGCCCTTTTTTGCCGGACTGCAGCGGCC GCTACTAGTATCATTACTTATACAGCTCGTCCATACCGTGGGTGATGCCCGCAGCGGTCA CAAATTCCAGCAGGACCATGTGATCACGTTTTTCGTTCGGATCTTTGCTCAGCACGCTTT GGGTGCTCAGATAGTGATTATCCGGCAGCAAAACCGGACCGTCACCAATTGGGGTGTTCT GCTGGTAATGGTCGGGTTAC

Retrieved from "http://2011.igem.org/Team:Imperial_College_London/Sequence"