Team:Paris Bettencourt/Experiments

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<h1>Experiments</h1>
<h1>Experiments</h1>
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<p>You can find here all the experiments we did in the lab! The data is organized by design and you will be able to find both the characterization steps and our diffusion experiments.</p>
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<h2>Preliminaries experiments</h2>
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  <td style="width:200px; text-align:center"><a href="https://2011.igem.org/Team:Paris_Bettencourt/Preliminaries"><img style="width:150px" src="https://static.igem.org/mediawiki/2011/8/80/GFP-diff-button.png"></a>
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  <td><b><a href="https://2011.igem.org/Team:Paris_Bettencourt/Preliminaries">Preliminaries experiments</a></b>: go back and see how we re-did the experiment of the original nanotubes paper.
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<h2>New devices</h2>
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<p>We designed entirely these new devices. They are usually composed of an emitter, a receptor and an amplifier sub-unit.</p>
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  <td style="width:200px; text-align:center"><a href="https://2011.igem.org/Team:Paris_Bettencourt/Experiments/YFP_TetR_diffusion"><img style="width:150px" src="https://static.igem.org/mediawiki/2011/d/d0/YFP_concentration_button.png"></a>
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  <td><b><a href="https://2011.igem.org/Team:Paris_Bettencourt/Experiments/YFP_TetR_diffusion">YFP concentrator</a></b> This design relies on a TetO-array which allow us to concentrate YFP-TetR fusion proteins.
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  <td style="width:200px; text-align:center;"><a href="https://2011.igem.org/Team:Paris_Bettencourt/Experiments/T7_diffusion"><img style="width:150px; margin-top:20px;" src="https://static.igem.org/mediawiki/2011/e/e4/T7_button.png"></a>
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  </td>
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  <td><b><a href="https://2011.igem.org/Team:Paris_Bettencourt/Experiments/T7_diffusion">T7 RNA polymerase diffusion</a></b> In this design, we introduce the use of the T7 polymerase both as the transfer molecule and as the auto-amplification system.
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  <td style="width:200px; text-align:center"><a href="https://2011.igem.org/Team:Paris_Bettencourt/Experiments/tRNA_diffusion"><img style="width:150px; margin-top:20px;" src="https://static.igem.org/mediawiki/2011/5/53/TRNAamber-button.png"></a>
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  </td>
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  <td><b><a href="https://2011.igem.org/Team:Paris_Bettencourt/Experiments/tRNA_diffusion">tRNA amber diffusion</a></b> The tRNA amber is the smallest molecule we are trying to get pass the nanotubes.
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</table>
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<h2>Using bistable switches</h2>
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<p>During our brainstormings, we noticed several natural or artificial bistable switches that could serve both as a receptor and an auto-amplifier. One molecule carefully chosen could toggle the switch in another position. All we have to do is see if it diffuses through the nanotubes.
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  <td style="width:200px; text-align:center"><a href="https://2011.igem.org/Team:Paris_Bettencourt/Experiments/ComS_diffusion"><img style="width:150px; margin-top:20px;" src="https://static.igem.org/mediawiki/2011/2/21/ComS-button.png"></a>
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  </td>
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  <td><b>"<a href="https://2011.igem.org/Team:Paris_Bettencourt/Experiments/ComS_diffusion">ComS diffusion</a></b> We took advantage of a switch already existing in <i>B.Subtilis</i> (the ComK/ComS switch) and tried to see if we could toggle it from one state to the other using molecules diffusing through the nanotubes.
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  </td>
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  <td style="width:200px; text-align:center"><a href="https://2011.igem.org/Team:Paris_Bettencourt/Experiments/SinOp"><img style="width:150px; margin-top:20px;" src="https://static.igem.org/mediawiki/2011/2/21/ComS-button.png"></a>
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  <td><b><a href="https://2011.igem.org/Team:Paris_Bettencourt/Experiments/SinOp">Sin Operon</a></b>
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  <td style="width:200px; text-align:center">Lambda switch<a href="https://2011.igem.org/Team:Paris_Bettencourt/Experiments/Lambda_switch"><img style="width:150px; margin-top:20px;" src="https://static.igem.org/mediawiki/2011/2/21/ComS-button.png"></a>
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  </td>
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  <td><b><a href="https://2011.igem.org/Team:Paris_Bettencourt/Experiments/Lambda_switch">Lambda switch</a></b>
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<div id="scroll_left"><a href="https://2011.igem.org/Team:Paris_Bettencourt/Designs"><img src="https://static.igem.org/mediawiki/2011/0/0a/Arrow-left-big.png" style="width:100%;"></a><a href="https://2011.igem.org/Team:Paris_Bettencourt/Designs">Go back to the designs!</a></div>
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<div id="scroll_left"><a href="https://2011.igem.org/Team:Paris_Bettencourt/Modeling"><img src="https://static.igem.org/mediawiki/2011/0/0a/Arrow-left-big.png" style="width:100%;"></a><a href="https://2011.igem.org/Team:Paris_Bettencourt/Modeling">Modeling overview</a></div>
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Latest revision as of 18:58, 27 October 2011

Team IGEM Paris 2011

Experiments

You can find here all the experiments we did in the lab! The data is organized by design and you will be able to find both the characterization steps and our diffusion experiments.

Preliminaries experiments

Preliminaries experiments: go back and see how we re-did the experiment of the original nanotubes paper.

New devices

We designed entirely these new devices. They are usually composed of an emitter, a receptor and an amplifier sub-unit.

YFP concentrator This design relies on a TetO-array which allow us to concentrate YFP-TetR fusion proteins.
T7 RNA polymerase diffusion In this design, we introduce the use of the T7 polymerase both as the transfer molecule and as the auto-amplification system.
tRNA amber diffusion The tRNA amber is the smallest molecule we are trying to get pass the nanotubes.

Using bistable switches

During our brainstormings, we noticed several natural or artificial bistable switches that could serve both as a receptor and an auto-amplifier. One molecule carefully chosen could toggle the switch in another position. All we have to do is see if it diffuses through the nanotubes.

"ComS diffusion We took advantage of a switch already existing in B.Subtilis (the ComK/ComS switch) and tried to see if we could toggle it from one state to the other using molecules diffusing through the nanotubes.
Sin Operon
Lambda switch Lambda switch

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