Team:UT Dallas/NotebookWeek2

From 2011.igem.org

(Difference between revisions)
 
(7 intermediate revisions not shown)
Line 398: Line 398:
   <div class="header">
   <div class="header">
     <div class="logo">
     <div class="logo">
-
       <h1><a href="index.html"><span>UTDallas</span> iGem 2011<small></small></a></h1>
+
       <h1><span>UTDallas</span> iGem<small></small></h1>
     </div>
     </div>
-
  <br></br>
+
   
     <div class="menu_nav">
     <div class="menu_nav">
-
<br></br>
+
 
       <ul>
       <ul>
         <li><a href="https://2011.igem.org/Team:UT_Dallas"><font size="3" face="verdana">Home</a></font></li>
         <li><a href="https://2011.igem.org/Team:UT_Dallas"><font size="3" face="verdana">Home</a></font></li>
Line 412: Line 412:
         <li class = "active"><a href="https://2011.igem.org/Team:UT_Dallas/Notebook"><font size="3" face="verdana">Notebook</a></font></li>
         <li class = "active"><a href="https://2011.igem.org/Team:UT_Dallas/Notebook"><font size="3" face="verdana">Notebook</a></font></li>
         <li><a href="https://2011.igem.org/Team:UT_Dallas/HumanPractices"><font size="3" face="verdana">Human Practices</a></font></li>
         <li><a href="https://2011.igem.org/Team:UT_Dallas/HumanPractices"><font size="3" face="verdana">Human Practices</a></font></li>
-
         <li><a href="https://2011.igem.org/Team:UT_Dallas/Gallery"><font size="3" face="verdana">Gallery</a></font></li>
+
         <li><a href="https://2011.igem.org/Team:UT_Dallas/Safety"><font size="3" face="verdana">Safety</a></font></li>
          
          
       </ul>
       </ul>
Line 428: Line 428:
         <div class="article">
         <div class="article">
           <h2><span> Week 2</span></h2>
           <h2><span> Week 2</span></h2>
-
           <p> <font size="3" face="verdana"></font></p>
+
           <p><font size = "5" face= "arial" color = "#184D79"><b>July 18-</b></font>
 +
              <blockquote><li type = "circle">Colony grew, negative control didn’t<li type = "circle">Transfomation- 50 µL DH5α, 2 µL DNA at 37 degrees Celsius overnight (all carb)<li type = "circle">Glycerol stock- 350 µL of cells and 150 µL of 50% glycerol<li type = "circle">Miniprep<li type = "circle">Dissolving and diluting primers (10 pmol/µL)<li type = "circle">PCR and gel electrophoresis and purification<li type = "circle">Digestion for 1 hour and 30 minutes at 37 degrees Celsius<li type = "circle">Gel Electrophoresis and Purification <li type = "circle">Digestion and ligation</blockquote>
 +
        <p><font size = "5" face= "arial" color = "#184D79"><b>July 19-</b></font>
 +
              <blockquote><li type = "circle">Transformation- 50 µL DH5α, 2 µL DNA at 37 degrees Celsius overnight</blockquote>
 +
 
 +
        <p><font size = "5" face= "arial" color = "#184D79"><b>July 20-</b></font>
 +
              <blockquote><li type = "circle">Incubate in 3 mL of LB and appropriate antibiotic overnight at 37 degrees Celsius and 220 rpm<li type = "circle">Ligation of Photo and YFP (i2.5.4 and i2.5.1) for 10 minutes at room temperature<li type = "circle">Transformation of Photo and YFP 50 µL DH5α, 2 µL DNA at 37 degrees Celsius overnight (all chlora)<li type = "circle">Making competent cells- 20 tubes (250 µL each) RP437, 20 tubes (250 µL each) BW2513</blockquote>
 +
 
 +
        <p><font size = "5" face= "arial" color = "#184D79"><b>July 21-</b></font>
 +
              <blockquote><li type = "circle">Glycerol stock- 150 µL of 50% glycerol and 350 µL of cells<li type = "circle">Miniprep<li type = "circle">Incubate in 3 mL LB and appropriate antibiotic overnight at 37 degrees Celsius and 220 rpm<li type = "circle">Test digestion for 1 hour and 20 minutes a 37 degrees Celsius<li type = "circle">- Test digestion for 1 hour and 20 minutes a 37 degrees Celsius</blockquote>
 +
 
 +
        <p><font size = "5" face= "arial" color = "#184D79"><b>July 22-</b></font>
 +
              <blockquote><li type = "circle">Glycerol Stock- 150 µL of 50% glycerol and 350 µL of cells<li type = "circle">Miniprep<li type = "circle">Glycerol stock- 350 µL of cells abd 150 µL of 50% glycerol<li type = "circle">Dissolving and Diluting primers<li type = "circle">Test digestion and gel electrophoresis </blockquote>
           <div class="clr"></div>
           <div class="clr"></div>
-
           <!--<img src="images/images_1.jpg" width="613" height="193" alt="" /><br></br> Add pictures, diagrams, and captions here.<br></br>
+
            
-
          <img src="images/images_1.jpg" width="613" height="193" alt="" /><br></br> Add pictures, diagrams, and captions here.<br></br>-->
+
                   <div class="clr"></div>
                   <div class="clr"></div>
Line 461: Line 472:
           <ul class="sb_menu">
           <ul class="sb_menu">
             <a href="https://2011.igem.org/Team:UT_Dallas/NotebookWeek1"><img src="https://static.igem.org/mediawiki/2011/9/95/W1.png" width="210" height="43"><br></br>
             <a href="https://2011.igem.org/Team:UT_Dallas/NotebookWeek1"><img src="https://static.igem.org/mediawiki/2011/9/95/W1.png" width="210" height="43"><br></br>
-
             <a href="https://2011.igem.org/Team:UT_Dallas/NotebookWeek2"><img src="https://static.igem.org/mediawiki/2011/6/65/W2.png" width="210" height="43"><br></br>
+
             <a href="https://2011.igem.org/Team:UT_Dallas/NotebookWeek2"><img src="https://static.igem.org/mediawiki/2011/5/58/W2Bold.png" width="210" height="43"><br></br>
             <a href="https://2011.igem.org/Team:UT_Dallas/NotebookWeek3"><img src="https://static.igem.org/mediawiki/2011/7/7f/W3.png" width="210" height="43"><br></br>
             <a href="https://2011.igem.org/Team:UT_Dallas/NotebookWeek3"><img src="https://static.igem.org/mediawiki/2011/7/7f/W3.png" width="210" height="43"><br></br>
             <a href="https://2011.igem.org/Team:UT_Dallas/NotebookWeek4"><img src="https://static.igem.org/mediawiki/2011/d/dd/W4.png" width="210" height="43"><br></br>
             <a href="https://2011.igem.org/Team:UT_Dallas/NotebookWeek4"><img src="https://static.igem.org/mediawiki/2011/d/dd/W4.png" width="210" height="43"><br></br>
Line 476: Line 487:
         </div></a>
         </div></a>
                       <div class="gadget">
                       <div class="gadget">
-
           <h2 class="grey"><span>Multimedia</span></h2>
+
            
           <div class="clr"></div>
           <div class="clr"></div>
           <div class="testi">
           <div class="testi">
-
             <p><span class="q"><img src="https://static.igem.org/mediawiki/2011/8/85/Quote_1.gif" width="16" height="14" alt="" /></span><script src="http://widgets.twimg.com/j/2/widget.js"></script>Twitter embed
+
              
-
<script>
+
-
</script><span class="q"><img src="https://static.igem.org/mediawiki/2011/a/a1/Quote_2.gif" width="16" height="14" alt="" /></span></p>
+
Line 497: Line 506:
" width="58" height="58" alt="" /></a> <a href="#"><img src="https://static.igem.org/mediawiki/2011/e/e8/Pic_3.jpg" width="58" height="58" alt="" /></a> <a href="#"><img src="https://static.igem.org/mediawiki/2011/2/2a/Pic_4.jpg" width="58" height="58" alt="" /></a> <a href="#"><img src="https://static.igem.org/mediawiki/2011/1/17/Pic_5.jpg" width="58" height="58" alt="" /></a> <a href="#"><img src="https://static.igem.org/mediawiki/2011/7/76/Pic_6.jpg" width="58" height="58" alt="" /></a> </div>
" width="58" height="58" alt="" /></a> <a href="#"><img src="https://static.igem.org/mediawiki/2011/e/e8/Pic_3.jpg" width="58" height="58" alt="" /></a> <a href="#"><img src="https://static.igem.org/mediawiki/2011/2/2a/Pic_4.jpg" width="58" height="58" alt="" /></a> <a href="#"><img src="https://static.igem.org/mediawiki/2011/1/17/Pic_5.jpg" width="58" height="58" alt="" /></a> <a href="#"><img src="https://static.igem.org/mediawiki/2011/7/76/Pic_6.jpg" width="58" height="58" alt="" /></a> </div>
     <div class="col c2">
     <div class="col c2">
-
       <h2><span><font size="5" face="verdana">UT Dallas</span></font></h2>
+
       <h2><span><font size="5" face="arial">UT Dallas</span></font></h2>
     <img src="https://static.igem.org/mediawiki/2011/a/ad/Nserl_Pic.png" width="112" height="138" alt="" ALIGN = RIGHT />     
     <img src="https://static.igem.org/mediawiki/2011/a/ad/Nserl_Pic.png" width="112" height="138" alt="" ALIGN = RIGHT />     
  <p>
  <p>
Line 503: Line 512:
     </div>
     </div>
     <div class="col c3">
     <div class="col c3">
-
       <h2><span><font size="5" face="verdana">Notebook</span></font></h2>
+
       <h2><span><font size="5" face="arial">Notebook</span></font></h2>
-
       <p></p><p> <a href="#"><font size="3" face="verdana">Learn more...</a></font></p>
+
       <p></p><p> <a href="https://2011.igem.org/Team:UT_Dallas/Notebook"><font size="3" face="verdana">Learn more...</a></font></p>
     </div>
     </div>

Latest revision as of 02:12, 3 September 2011

biz solution

Week 2

July 18-

  • Colony grew, negative control didn’t
  • Transfomation- 50 µL DH5α, 2 µL DNA at 37 degrees Celsius overnight (all carb)
  • Glycerol stock- 350 µL of cells and 150 µL of 50% glycerol
  • Miniprep
  • Dissolving and diluting primers (10 pmol/µL)
  • PCR and gel electrophoresis and purification
  • Digestion for 1 hour and 30 minutes at 37 degrees Celsius
  • Gel Electrophoresis and Purification
  • Digestion and ligation

    • July 19-

  • Transformation- 50 µL DH5α, 2 µL DNA at 37 degrees Celsius overnight

    • July 20-

  • Incubate in 3 mL of LB and appropriate antibiotic overnight at 37 degrees Celsius and 220 rpm
  • Ligation of Photo and YFP (i2.5.4 and i2.5.1) for 10 minutes at room temperature
  • Transformation of Photo and YFP 50 µL DH5α, 2 µL DNA at 37 degrees Celsius overnight (all chlora)
  • Making competent cells- 20 tubes (250 µL each) RP437, 20 tubes (250 µL each) BW2513

    • July 21-

  • Glycerol stock- 150 µL of 50% glycerol and 350 µL of cells
  • Miniprep
  • Incubate in 3 mL LB and appropriate antibiotic overnight at 37 degrees Celsius and 220 rpm
  • Test digestion for 1 hour and 20 minutes a 37 degrees Celsius
  • - Test digestion for 1 hour and 20 minutes a 37 degrees Celsius

    • July 22-

  • Glycerol Stock- 150 µL of 50% glycerol and 350 µL of cells
  • Miniprep
  • Glycerol stock- 350 µL of cells abd 150 µL of 50% glycerol
  • Dissolving and Diluting primers
  • Test digestion and gel electrophoresis

    • Weeks 1-12

    Image Gallery

    UT Dallas

    Click here to learn more  about UTD

    Notebook

    Learn more...

    Retrieved from "http://2011.igem.org/Team:UT_Dallas/NotebookWeek2"