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Team:Edinburgh/Data
From 2011.igem.org
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==Phage Display System== | ==Phage Display System== | ||
| - | [[Image:Edinburgh-Data-Phage-Display.png|thumb|center|710px|The completed system should contain:<br> | + | [[Image:Edinburgh-Data-Phage-Display.png|thumb|center|710px|The completed system should contain:<br> |
A '''promoter''' (<partinfo>BBa_K523000</partinfo>) controlling:<br> | A '''promoter''' (<partinfo>BBa_K523000</partinfo>) controlling:<br> | ||
an '''Endoglucanase—pVIII''' fusion<br> | an '''Endoglucanase—pVIII''' fusion<br> | ||
Revision as of 19:36, 2 September 2011
Data Page
The iGEM rules require us to have simple illustrations of how our devices work and where the Parts function in the system; and links to the Registry for the parts/constructs for which we have produced data.
See the sample Data page.
Cell Surface Display System
A promoter (<partinfo>BBa_K523000</partinfo>) controlling:
an INP—Endoglucanase fusion (<partinfo>BBa_K523008</partinfo> + <partinfo>BBa_K523011</partinfo>)
an INP—β-glucosidase fusion (<partinfo>BBa_K523008</partinfo> + <partinfo>BBa_K523010</partinfo>)
an INP—Exoglucanase fusion (<partinfo>BBa_K523008</partinfo> + <partinfo>BBa_K523009</partinfo>)
Ribosome Binding Sites are indicated as green ovals.
Cellulose degradation is shown at top. In reality, tens of thousands of enzymes will cover the outer membrane in random places.
A test system to prove that <partinfo>BBa_K523008</partinfo> can be used to carry proteins to the outer membrane uses a fusion to Yellow Fluorescent Protein (YFP) or the E. coli amylase "malS" instead.
Phage Display System
A promoter (<partinfo>BBa_K523000</partinfo>) controlling:
an Endoglucanase—pVIII fusion
a β-glucosidase—pVIII fusion
an Exoglucanase—pVIII fusion
Ribosome Binding Sites are indicated as green ovals. "Signal" means a periplasmic signal sequence, directing the protein to the periplasm to be assembled into the phage.
A test system uses a fusion of pVIII to E. coli amylase "malS" instead.
