Team:Freiburg/Notebook/25 August

From 2011.igem.org

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The precipitator was cloned into pSB1K3 and pSB1T3.
The precipitator was cloned into pSB1K3 and pSB1T3.
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===Miniprep===
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'''Investigators: Sandra'''
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continue from Transformation; 22.08.11; Investigator: Sophie
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Revision as of 15:55, 25 August 2011


This is the wiki page
of the Freiburger student
team competing for iGEM 2011.
Thank you for your interest!

Contents

Commons

PCR of pSB1A3 and pSB1C3

Investigators: Sandra

Primer:

  • P35
  • P36

Testdigest:

Expected bands:
pSB1A3: 2155 bp

pSB1C3: 2070 bp

green light receptor

NAME OF YOUR EXPERIMENT

Investigators:NAME

blue light receptor

Miniprep

Investigators: Sandra


Miniprep of LovTAP-NotGate-pSB1A3.

After Miniprep the samples were digested with:

  • EcoRI
  • PstI

The testdigest showed no bands!

Trafo

Investigators: Sandra

Transformation of LovTAP-NotGate-pSB1A3 again, because the last transformation did not show good colonies to pick. Incubation over night.

Clones need to be picked tomorrow.

3A-assembly:Digestion

Investigators: Sandra

Digestion of:

  • Not
  • nOt
  • noT
  • Lovtap 3A
  • pSB1A3


red light receptor

NAME OF YOUR EXPERIMENT

Investigators:NAME



Lysis cassette

NAME OF YOUR EXPERIMENT

Investigators:NAME


Precipitator

Insertion of the precipitator into a iGEM vector

Investigators: Julia
The precipitator was cloned into pSB1K3 and pSB1T3.

Miniprep

Investigators: Sandra
continue from Transformation; 22.08.11; Investigator: Sophie

                       

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