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Team:Northwestern/Notebook/Protocols/Overnight
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*Take 5mL LB and put in a round bottom 5mL tube. | *Take 5mL LB and put in a round bottom 5mL tube. | ||
*If your antibiotic is 1000x (which it should be) add 5uL antibiotic to your tube. Make sure you add the right one! | *If your antibiotic is 1000x (which it should be) add 5uL antibiotic to your tube. Make sure you add the right one! | ||
| - | *Take | + | *Take a p200 tip and lightly touch a colony. |
*Eject the tip into the culture and put the cap on. | *Eject the tip into the culture and put the cap on. | ||
| - | *For a miniprep, grow overnight for 12-16 hours | + | *For a miniprep, grow overnight for 12-16 hours. |
| + | *Left over overnight cultures (after miniprepping) can be used to make [[Team:Northwestern/Notebook/Protocols/Glycerol|glycerol stocks]]. | ||
Latest revision as of 20:17, 23 August 2011
PROJECT
RESULTS
CONSIDERATIONS
ABOUT US
NOTEBOOK
ATTRIBUTIONS
Overnight
- Take 5mL LB and put in a round bottom 5mL tube.
- If your antibiotic is 1000x (which it should be) add 5uL antibiotic to your tube. Make sure you add the right one!
- Take a p200 tip and lightly touch a colony.
- Eject the tip into the culture and put the cap on.
- For a miniprep, grow overnight for 12-16 hours.
- Left over overnight cultures (after miniprepping) can be used to make glycerol stocks.
REMEMBER TO USE PROPER STERILE TECHNIQUE
Adapted from [http://openwetware.org/wiki/Bacterial_cell_culture OpenWetWare]
