Latest revision as of 20:17, 23 August 2011

PROJECT

Overnight

Take 5mL LB and put in a round bottom 5mL tube.
If your antibiotic is 1000x (which it should be) add 5uL antibiotic to your tube. Make sure you add the right one!
Take a p200 tip and lightly touch a colony.
Eject the tip into the culture and put the cap on.
For a miniprep, grow overnight for 12-16 hours.
Left over overnight cultures (after miniprepping) can be used to make glycerol stocks.

REMEMBER TO USE PROPER STERILE TECHNIQUE

Adapted from [http://openwetware.org/wiki/Bacterial_cell_culture OpenWetWare]

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-*Take 5mL LB and put in a round bottom tube
-*If your antibiotic is 1000x (which it should be) add 5uL antibiotic to your tube
+*Take 5mL LB and put in a round bottom 5mL tube.
-   *'''MAKE SURE YOU ADD THE RIGHT ONE!'''
+*If your antibiotic is 1000x (which it should be) add 5uL antibiotic to your tube. Make sure you add the right one!
-*Take a tip on a p200 and lightly touch a colony
+*Take a p200 tip and lightly touch a colony.
-*Eject the tip into the culture and put the cap on
+*Eject the tip into the culture and put the cap on.
-*For a miniprep, grow O/N for 12-16 hours NOT LONGER
+*For a miniprep, grow overnight for 12-16 hours.
-[http://openwetware.org/wiki/Bacterial_cell_culture]Openwetware
+*Left over overnight cultures (after miniprepping) can be used to make [[Team:Northwestern/Notebook/Protocols/Glycerol|glycerol stocks]].
 '''REMEMBER TO USE PROPER STERILE TECHNIQUE'''
+Adapted from [http://openwetware.org/wiki/Bacterial_cell_culture OpenWetWare]