Team:Edinburgh/Phage Replication

M13 Replication

• The M13 phage attacks E.coli(host), multiplies in the host cell cytoplasm, and is released without causing the bacteria’s death(non-lytic).

Basic model

• dx/dt=ax-bvx （Rate of change of quantity of uninfected E.coli equals to the uninfected E.coli replicate itself minus the E.coli infected by M13 phage.）

• dy/dt=ay+bvx （Rate of change of quantity of infected E.coli equals to the quantity of infected E.coli replicate itself (The change of life circulation of E.coli after being infected is not considered here.) plus the E.coli infected by M13 phage.）

• dv/dt=cy-bvx-mv （Rate of change of quantity of free phage equals to the phage released by infected E.coli minus the phage which is to infect an E.coli and the decayed phage.）

X(t) uninfected E.coli Y(t) infected E.coli V(t) free phage a replication coefficient of E.coli b transmission coefficient of phage c replication coefficient of phage m decay rate of phage

References

• [http://www.wwnorton.com/college/biology/microbiology2/ch/11/etopics.aspx molecular overview of M13]