Team:UNICAMP-EMSE Brazil/protocols/Digestion

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{{Template:UNICAMP-EMSE Brazil_Main_Menu}}
{{Template:UNICAMP-EMSE Brazil_Main_Menu}}
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<div style="text-align: right;">
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<font size="4" color="red">Back to [https://2011.igem.org/Team:UNICAMP-EMSE_Brazil/protocols Protocols ↑]</font>
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==Digestion==
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[[Image:UNICAMP-EMSE_Brazil_Restriction.png|270px|right]]
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==<font size="5" color=#086A87>Digestion:==
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(For each 1000 ng of DNA use 1 U of enzyme in 20uL reaction)
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(For each 1000 ng of DNA use 1 U of enzyme in 20µL reaction)
#Use 3000 ng BioBrick vector  
#Use 3000 ng BioBrick vector  
#6 µL 10x buffer
#6 µL 10x buffer
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#distilled water to 60 µL total volume
#distilled water to 60 µL total volume
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Put in 0,2 mL eppendorf tubes and let 2 h at 37°C.
 
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Make an electrophoresys (use the hole reaction volume) and cut the band with your gene of interest.
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*'''Put in 0,2 mL eppendorf tubes and let 2 h at 37°C.'''
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*'''Make an electrophoresis (use the hole reaction volume) and cut the band with your gene of interest.'''
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Q[0] = "<i>I’m Luke Skywalker, I’m here to rescue you.</i>Luke, Star Wars: Episode IV-A New Hope (1977)"
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Q[1] = "<i>Watch your mouth kid, or you’ll find yourself floating home.</i>Star Wars: Episode IV-A New Hope (1977)"
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Q[2] = "<i>Evacuate in our moment of triumph? I think you overestimate their chances.</i> Star Wars: Episode IV-A New Hope (1977)"
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Q[3] = "<i>The Force is strong with this one.</i> Star Wars: Episode IV-A New Hope (1977)"
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Q[4] = "<i> I have you now!</i> Darth Vader, Star Wars: Episode IV-A New Hope (1977)"
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Q[5] = "<i> Fear is the path to the dark side. Fear leads to anger, anger leads to hate, hate leads to suffering.</i> MsC Yoda, Star Wars Episode I: The Phantom Menace (1999)"
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Q[6] = "<i> No blasters! No blasters!.</i> A bartender, Star Wars: Episode IV-A New Hope (1977)"
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Q[7] = "<i> Go to the center of the gravity's pull, and find your planet you will...</i> M.Sc. Yoda, Star Wars Episode II: Attack of the Clones (2002)"
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Q[8] = "<i> Clear, your mind must be if you are to discover the real villains behind the plot.</i> M.Sc. Yoda, Star Wars Episode II: Attack of the Clones (2002)"
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Q[9] = "<i> Much to learn, you still have.</i> M.Sc. Yoda, Star Wars Episode II: Attack of the Clones (2002)"
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Q[10] = "<i> Meditate on this, I will.</i> M.Sc. Yoda, Star Wars Episode II: Attack of the Clones (2002)"
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Q[11] = "<i> Use the Force, Luke.</i> M.Sc. Yoda, Star Wars Episode II: Attack of the Clones (2002)"
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Q[12] = "<i> May the Force be with you.</i> Mentioned several times throughout the Star Wars saga"
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Q[13] = "<i> I really need a Jedi Bacteria.</i> Iolanda Albuquerque, UNICAMP-EMSE team advisor"
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Q[14] = "<i> Another splendid day in Brazil!!!</i> Marc Emery, UNICAMP-EMSE team student"
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Q[15] = "<i> Dont' cut, let your hair volume expressing all their feelings...</i> Thibault Sabattier (UNICAMP-EMSE team student) about Nemailla's hair"
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Q[16] = "<i> What a good life!!</i> Louise Marais (UNICAMP-EMSE team student) about living in Brazil"
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Q[17] = "<i> Gosh!I will have nigthmares tonight! </i> Danieli Gonçalves (UNICAMP-EMSE team student) about Thibault wearing Marianna's dress"
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Q[18] = "<i> I will revenge myself against you and Marc Emery!!!</i> Nemailla Bonturi (UNICAMP-EMSE team advisor)"
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Q[19] = "<i> Yeah, those dresses deserve us!</i> Marc Emery (UNICAMP-EMSE team student) about Marianna's dresses"
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Q[20] = "<i> I am really starting getting worried with you guys!!</i> Iolanda Albuquerque (UNICAMP-EMSE team advisor)"
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Latest revision as of 00:58, 23 August 2011

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UNICAMP-EMSE Brazil Restriction.png

Digestion:

(For each 1000 ng of DNA use 1 U of enzyme in 20µL reaction)

  1. Use 3000 ng BioBrick vector
  2. 6 µL 10x buffer
  3. 0.5 µL enzyme 1 (20 units/µL)
  4. 0.5 µL enzyme 2 (20 units/µL)
  5. distilled water to 60 µL total volume


  • Put in 0,2 mL eppendorf tubes and let 2 h at 37°C.
  • Make an electrophoresis (use the hole reaction volume) and cut the band with your gene of interest.






Retrieved from "http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/protocols/Digestion"