Team:Cornell/Week 12
From 2011.igem.org
(Difference between revisions)
(→Sunday, August 21) |
Rockyshell92 (Talk | contribs) (→Monday) |
||
Line 27: | Line 27: | ||
==Monday== | ==Monday== | ||
+ | Morning lab work done by: Youjin Cho | ||
+ | :'''Objective''' | ||
+ | ::*Miniprep the vioB+avi-tag+pZE12 samples and check sequencing. | ||
+ | ::'''Miniprep and Sequencing''' | ||
+ | :::*Miniprepped the vioB+avi-tag+pZE12 (1,2,3) samples using standard Qiagen Miniprep protocol. | ||
+ | :::*Using 1μL of pZE12 reverse primer dilution and 17μL of each template, prepared and submitted the samples for sequencing. | ||
+ | ::::<u>Order number: 10255430</u> | ||
==Tuesday== | ==Tuesday== |
Revision as of 15:27, 22 August 2011
Week 1 | Week 2 - 5 | Week 6 | Week 7 | Week 8 | Week 9 | Week 10 | Week 11 | Week 12 | Week 13 | Week 14 | Week 15 | Week 16 | Week 17 | Week 18 | Week 19 | Week 20 | Week 21 |
August 21st - August 27th
Sunday, August 21
Evening lab work done by: Youjin Cho, Charlie Chung
- Picking Colonies and Starting Up Cultures of vioB Ligation Reaction
- (Avi-Tagged pZE12 backbone) control plate shows no growth of colonies
- (vioB + Avi-Tagged pZE12 backbone) plate shows numerous colonies
- - Picked three colonies (vioB+Avi+BB #1, 2, 3) and started 3mL cultures in LB with 3μL ampicillin
- - Incubated overnight in the 37°C shaker of Room 304
- PCR Reaction Setup of GFP (because sequencing results were negative)
- 38.5μL ddH2O
- 5μL 10x buffer
- 2.5μL dNTP
- 1μL forward GFP primer
- 1μL reverse GFP primer
- 1μL (pZE12+GFP) backbone template
- 1μL Vent DNA polymerase
- 50μL Total
- Annealing temperature of 54.2°C
- 1 minute elongation phase
Monday
Morning lab work done by: Youjin Cho
- Objective
- Miniprep the vioB+avi-tag+pZE12 samples and check sequencing.
- Miniprep and Sequencing
- Miniprepped the vioB+avi-tag+pZE12 (1,2,3) samples using standard Qiagen Miniprep protocol.
- Using 1μL of pZE12 reverse primer dilution and 17μL of each template, prepared and submitted the samples for sequencing.
- Order number: 10255430