Team:Glasgow/Pattern Forming Device

From 2011.igem.org

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<h1>Construct Overview</h1>
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<h1>Pattern Forming Device Construct</h1>
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<p>Here is a short sentence describing the construct's function, to see how each part works please interact with the image below.</p>
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<p>The pattern forming device essentially causes targeted dispersal of biofilm. To target the dispersal a light dependant promoter is used allowing bio-photo-lithography.</p>
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   <td id="leftcolumn" width="148" align="left" valign="top"><p><b>Blue Light Dependant Promoter</b>
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   <td id="leftcolumn" width="122" align="center" valign="top"><p><b>Blue Light Dependant Promoter</b>
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       <br /><a href="http://partsregistry.org/Part:BBa_K238013">BBa_K238013</a></p></td>
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       <br /><a href="http://partsregistry.org/Part:BBa_K238013">BBa_K238013</a></p>
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   <td id="leftcolumn" width="210" align="left" valign="top"><p><b>Strong Ribosome Binding Site</b>
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      <p>Weak blue light dependant promoter. Strength is considered reletive to I20260
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       <br /><a href="http://partsregistry.org/Part:BBa_B0030">BBa_B0030</a></p></td>
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   <td id="leftcolumn" width="195" align="center" valign="top"><p><b>Strong Ribosome Binding Site</b>
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   <td id="leftcolumn" width="157" align="left" valign="top"><p><b>Colicin E2</b>
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       <br /><br /><a href="http://partsregistry.org/Part:BBa_B0030">BBa_B0030</a></p>
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       <br /><a href="http://partsregistry.org/Part:BBa_K131009">BBa_K131009</a></p></td>
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      <p>Strong RBS based on Ron Weiss thesis. Strength is considered relative to BBa_B0031, BBa_B0032, BBa_B0033 and BBa_B0034</p></td>
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   <td id="leftcolumn" width="171" align="left" valign="top"><p><b>Double Terminator</b>
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   <td id="leftcolumn" width="165" align="center" valign="top"><p><b>Colicin E2</b>
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       <br /><a href="http://partsregistry.org/Part:BBa_B1006">BBa_B1006</a></p></td>
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       <br /><br /><a href="http://partsregistry.org/Part:BBa_K131009">BBa_K131009</a></p>
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      <p>The Colicin E2 operon without the SOS promoter. Colicin has DNAse activity.</p>
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      <p><b>-OR-</b>
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      <p><a href="https://2011.igem.org/Team:Glasgow/c-di-GMP Phosphodiesterase"><b>c-di-GMP Phosphodiesterase</b></a>
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      <br />Novel biobrick</p>
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      <p><b>-OR-</b></p>
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      <p><a href="https://2011.igem.org/Team:Glasgow/Ranaspumin"><b>Ranaspumin</b></a>
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      <br />Novel biobrick</p>
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      <p><b>-OR-</b></p>
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      <p><a href="https://2011.igem.org/Team:Glasgow/Latherin"><b>Latherin</b></a>
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      <br />Novel biobrick</p></td>
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   <td id="leftcolumn" width="150" align="center" valign="top"><p><b>Double Terminator</b>
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       <br /><br /><a href="http://partsregistry.org/Part:BBa_B1006">BBa_B1006</a></p>
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      <p>Artificial double terminator with 8bp stem and 6 nucleotide loop</p></td>
</tr>   
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</table>
</table>

Latest revision as of 14:30, 12 August 2011


Pattern Forming Device Construct

The pattern forming device essentially causes targeted dispersal of biofilm. To target the dispersal a light dependant promoter is used allowing bio-photo-lithography.

Blue Light Dependant Promoter
BBa_K238013

Weak blue light dependant promoter. Strength is considered reletive to I20260

Strong Ribosome Binding Site

BBa_B0030

Strong RBS based on Ron Weiss thesis. Strength is considered relative to BBa_B0031, BBa_B0032, BBa_B0033 and BBa_B0034

Colicin E2

BBa_K131009

The Colicin E2 operon without the SOS promoter. Colicin has DNAse activity.

-OR-

c-di-GMP Phosphodiesterase
Novel biobrick

-OR-

Ranaspumin
Novel biobrick

-OR-

Latherin
Novel biobrick

Double Terminator

BBa_B1006

Artificial double terminator with 8bp stem and 6 nucleotide loop

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