Team:Glasgow/Pattern Forming Device

From 2011.igem.org


Pattern Forming Device Construct

The pattern forming device essentially causes targeted dispersal of biofilm. To target the dispersal a light dependant promoter is used allowing bio-photo-lithography.

Blue Light Dependant Promoter
BBa_K238013

Weak blue light dependant promoter. Strength is considered reletive to I20260

Strong Ribosome Binding Site

BBa_B0030

Strong RBS based on Ron Weiss thesis. Strength is considered relative to BBa_B0031, BBa_B0032, BBa_B0033 and BBa_B0034

Colicin E2

BBa_K131009

The Colicin E2 operon without the SOS promoter. Colicin has DNAse activity.

-OR-

c-di-GMP Phosphodiesterase
Novel biobrick

-OR-

Ranaspumin
Novel biobrick

-OR-

Latherin
Novel biobrick

Double Terminator

BBa_B1006

Artificial double terminator with 8bp stem and 6 nucleotide loop