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Team:EPF-Lausanne/Protocols/Transformation
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{{:Team:EPF-Lausanne/Templates/ProtocolHeader|title=Transformation}} | {{:Team:EPF-Lausanne/Templates/ProtocolHeader|title=Transformation}} | ||
| - | Transformation is the introduction of a foreign plasmid into competent cells. We follow the protocol from last year's team: [https://static.igem.org/mediawiki/2010/1/1e/Prot_transformation.pdf | + | Transformation is the introduction of a foreign plasmid into competent cells. We follow the protocol from last year's team: [https://static.igem.org/mediawiki/2010/1/1e/Prot_transformation.pdf 2010 protocol] |
== Additional notes == | == Additional notes == | ||
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** For relatively pure plasmids, 1 ul is enough. | ** For relatively pure plasmids, 1 ul is enough. | ||
** For low concentration plasmids, like those originating from a Gibson assembly, use 2 to 3 ul. | ** For low concentration plasmids, like those originating from a Gibson assembly, use 2 to 3 ul. | ||
| + | ** If you are conducting the heat shock in an eppendorf tube, use 45 seconds instead of 30. | ||
{{:Team:EPF-Lausanne/Templates/Footer}} | {{:Team:EPF-Lausanne/Templates/Footer}} | ||
Latest revision as of 12:47, 11 August 2011
Transformation
Back to protocols.Transformation is the introduction of a foreign plasmid into competent cells. We follow the protocol from last year's team: 2010 protocol
Additional notes
- The protocol specifies to ask Henrike for plasmid quantities. Here is her answer:
- For relatively pure plasmids, 1 ul is enough.
- For low concentration plasmids, like those originating from a Gibson assembly, use 2 to 3 ul.
- If you are conducting the heat shock in an eppendorf tube, use 45 seconds instead of 30.
