Team:Freiburg/Notebook/10 August

From 2011.igem.org

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|'''1 pTb'''
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|1 L23
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|'''1 L23'''
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Revision as of 12:14, 10 August 2011


This is the wiki page
of the Freiburger student
team competing for iGEM 2011.
Thank you for your interest!

Contents

green light receptor

NAME OF YOUR EXPERIMENT

Investigators:NAME


blue light receptor

NAME OF YOUR EXPERIMENT

Investigators:NAME



red light receptor

3A-assembly

Investigators: Sandra

Digestion

  • PR1 (pSB1C3)
  • PR2 (pSB1C3)
  • pcyA (pSB1T3)
  1. pcyATd (pSB1T3)
  2. pcyATb (pSB1T3)
  • L23 (pSB1T3)
  • vector (pSB1K3)


Amount of DNA and H20:

Sample 500(ng)/DNA concentration (ng/μl) H20 μl
pcyATd 2.8 35.2
pcyaTb 2.9 35.1
PR2 7.2 30.8
PR1 6.1 31.9
L23 4.8 33.2
pSB1K3 20 18


Enzymes necessary for digestion:

PR1/PR2 pcyA/L23 vector
enzyme 1 EcoRI XbaI EcoRI
enzyme 2 SpeI PstI PstI


-incubated for 1 hours at 37°C.
-heated for 20 minutes at 80°C


Ligation

Ligation of:

PcayTd PcyATb L23 (ho1)
PR1 1 pTd 1 pTb 1 L23
PR2 2 pTd 2 pTb 2 L23

Lysis cassette

NAME OF YOUR EXPERIMENT

Investigators:NAME


Precipitator

NAME OF YOUR EXPERIMENT

Investigators: NAME

                       

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