Team:Freiburg/Notebook/10 August

From 2011.igem.org

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'''Ligation'''
'''Ligation'''
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Ligation of:
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{|cellpadding="10" cellspacing="0" border="1"
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|
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|PcayTd
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|PcyATb
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|L23 (ho1)
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|-
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|PR1
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|1pTd
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|1pTb
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|1L23
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|-
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|PR2
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|2pTd
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|2pTb
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|2L23
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|}
==<span style="color:orange;">Lysis cassette</span>==
==<span style="color:orange;">Lysis cassette</span>==

Revision as of 12:12, 10 August 2011


This is the wiki page
of the Freiburger student
team competing for iGEM 2011.
Thank you for your interest!

Contents

green light receptor

NAME OF YOUR EXPERIMENT

Investigators:NAME


blue light receptor

NAME OF YOUR EXPERIMENT

Investigators:NAME



red light receptor

3A-assembly

Investigators: Sandra

Digestion

  • PR1 (pSB1C3)
  • PR2 (pSB1C3)
  • pcyA (pSB1T3)
  1. pcyATd (pSB1T3)
  2. pcyATb (pSB1T3)
  • L23 (pSB1T3)
  • vector (pSB1K3)


Amount of DNA and H20:

Sample 500(ng)/DNA concentration (ng/μl) H20 μl
pcyATd 2.8 35.2
pcyaTb 2.9 35.1
PR2 7.2 30.8
PR1 6.1 31.9
L23 4.8 33.2
pSB1K3 20 18


Enzymes necessary for digestion:

PR1/PR2 pcyA/L23 vector
enzyme 1 EcoRI XbaI EcoRI
enzyme 2 SpeI PstI PstI


-incubated for 1 hours at 37°C.
-heated for 20 minutes at 80°C


Ligation

Ligation of:

PcayTd PcyATb L23 (ho1)
PR1 1pTd 1pTb 1L23
PR2 2pTd 2pTb 2L23

Lysis cassette

NAME OF YOUR EXPERIMENT

Investigators:NAME


Precipitator

NAME OF YOUR EXPERIMENT

Investigators: NAME

                       

Retrieved from "http://2011.igem.org/Team:Freiburg/Notebook/10_August"