"
Team:EPF-Lausanne
From 2011.igem.org
(→Project summary) |
(→Project summary) |
||
| Line 10: | Line 10: | ||
Our goal is to design transcription factors (TFs) that bind to new sequences, currently unavailable to synthetic biologists. These would allow to build larger genetic circuits than currently possible, with more independently regulated genes. The new transcription factors will be derived from tetR, one of the better characterised TFs out there. To characterise the new ones, we will introduce the tetR mutants and their corresponding mutant promoters into the circuit illustrated below, containing a LacI inverter and a reporter gene. The reporter for ''in vitro'' experiments is a fluorescence gene such as RFP, and a lysis system for ''in vivo'' experiments. The latter lyses cells with effective transcription factors, so that exclusively their DNA can be recovered. | Our goal is to design transcription factors (TFs) that bind to new sequences, currently unavailable to synthetic biologists. These would allow to build larger genetic circuits than currently possible, with more independently regulated genes. The new transcription factors will be derived from tetR, one of the better characterised TFs out there. To characterise the new ones, we will introduce the tetR mutants and their corresponding mutant promoters into the circuit illustrated below, containing a LacI inverter and a reporter gene. The reporter for ''in vitro'' experiments is a fluorescence gene such as RFP, and a lysis system for ''in vivo'' experiments. The latter lyses cells with effective transcription factors, so that exclusively their DNA can be recovered. | ||
| - | [[File:EPFL_Summary_(with_TFs).png| | + | [[File:EPFL_Summary_(with_TFs).png|650px]] |
<html> <!-- | <html> <!-- | ||
Revision as of 11:43, 5 August 2011
Welcome
It's on! After some warmup experiments during the semester, our team is now fully operational, and already having trouble getting away from the lab. Follow our adventures on twitter: IGEM_EPFL
Team, remember the Todo List
Project summary
Our goal is to design transcription factors (TFs) that bind to new sequences, currently unavailable to synthetic biologists. These would allow to build larger genetic circuits than currently possible, with more independently regulated genes. The new transcription factors will be derived from tetR, one of the better characterised TFs out there. To characterise the new ones, we will introduce the tetR mutants and their corresponding mutant promoters into the circuit illustrated below, containing a LacI inverter and a reporter gene. The reporter for in vitro experiments is a fluorescence gene such as RFP, and a lysis system for in vivo experiments. The latter lyses cells with effective transcription factors, so that exclusively their DNA can be recovered.
.png)
