"

From 2011.igem.org

Revision as of 09:30, 16 May 2011 (view source) Asterisk (Talk | contribs) ← Older edit		Revision as of 09:30, 16 May 2011 (view source) Asterisk (Talk | contribs) Newer edit →
Line 1:		Line 1:
	== PDMS two layer device fabrication ==		== PDMS two layer device fabrication ==
	made on 16.05.2011		made on 16.05.2011
		+
	<br>		<br>
-	'''''Materials:	+
		+	<p>'''''Materials: </p>
	----		----
	<p>    </p>		<p>    </p>

---

Revision as of 09:30, 16 May 2011

PDMS two layer device fabrication

made on 16.05.2011

Materials:

---

• flow and control layer molds

• Sylgard 184 silicone elastomer kit: Base part and a Curing agent

• TMCS

Method:

---

• Place molds into a TMCS vapor chamber

• Control layer mixture: 20g Base + 4g Curing agent

• Mix for 1 minute, degas for 2 minutes (standard protocol)

• pour onto control layer mold and place mold in vacuum chamber

• Flow layer mixture: 20g Base + 1g Curing agent

• Mix for 1 minute and degas for 2 minutes (standard protocol)

• Spin coat onto flow layer at 2200-2400rpm for 35secs (so far you can use my protocol on a spin coater)

• Remove control layer mold from vacuum chamber, making sure no bubbles are left on the surface (remove with a toothpick if you see some )

• Place the control and flow layer in a 80C convection oven and incubate for 30 minutes (timing is critical here!)

• Remove casts from oven, cut out control layer, punch holes, and align to flow layer

• Put aligned device back into 80C oven and incubate for at least 90 minutes (and here you can increase the backing)

• Remove devices from oven and punch flow layer holes