"
Team:EPF-Lausanne/Protocols
From 2011.igem.org
| Line 4: | Line 4: | ||
''Materials: | ''Materials: | ||
| - | • flow and control layer molds | + | <p>• flow and control layer molds</p> |
| - | • Sylgard 184 silicone elastomer kit: Base part and a Curing agent | + | <p>• Sylgard 184 silicone elastomer kit: Base part and a Curing agent</p> |
| - | • TMCS | + | <p>• TMCS</p> |
''Method: | ''Method: | ||
| - | • Place molds into a TMCS vapor chamber/ | + | <p>• Place molds into a TMCS vapor chamber</p> |
| - | • Control layer mixture: 20g Base + 4g Curing agent | + | <p>• Control layer mixture: 20g Base + 4g Curing agent</p> |
| - | • Mix for 1 minute, degas for 2 minutes (standard protocol) | + | <p>• Mix for 1 minute, degas for 2 minutes (standard protocol)</p> |
| - | • pour onto control layer mold and place mold in vacuum chamber | + | <p>• pour onto control layer mold and place mold in vacuum chamber</p> |
| - | • Flow layer mixture: 20g Base + 1g Curing agent | + | <p>• Flow layer mixture: 20g Base + 1g Curing agent</p> |
| - | • Mix for 1 minute and degas for 2 minutes (standard protocol) | + | <p>• Mix for 1 minute and degas for 2 minutes (standard protocol)</p> |
| - | • Spin coat onto flow layer at 2200-2400rpm for 35secs (so far you can use my protocol on a spin coater) | + | <p>• Spin coat onto flow layer at 2200-2400rpm for 35secs (so far you can use my protocol on a spin coater)</p> |
| - | • Remove control layer mold from vacuum chamber, making sure no bubbles are left on the surface (remove with a toothpick if you see some ) | + | <p>• Remove control layer mold from vacuum chamber, making sure no bubbles are left on the surface (remove with a toothpick if you see some )</p> |
| - | • Place the control and flow layer in a 80C convection oven and incubate for 30 minutes (timing is critical here!) | + | <p>• Place the control and flow layer in a 80C convection oven and incubate for 30 minutes (timing is critical here!)</p> |
| - | • Remove casts from oven, cut out control layer, punch holes, and align to flow layer | + | <p>• Remove casts from oven, cut out control layer, punch holes, and align to flow layer</p> |
| - | • Put aligned device back into 80C oven and incubate for at least 90 minutes (and here you can increase the backing) | + | <p>• Put aligned device back into 80C oven and incubate for at least 90 minutes (and here you can increase the backing)</p> |
| - | • Remove devices from oven and punch flow layer holes | + | <p>• Remove devices from oven and punch flow layer holes</p> |
Revision as of 09:24, 16 May 2011
PDMS two layer device fabrication
made on 16.05.2011
Materials:
• flow and control layer molds
• Sylgard 184 silicone elastomer kit: Base part and a Curing agent
• TMCS
Method:
• Place molds into a TMCS vapor chamber
• Control layer mixture: 20g Base + 4g Curing agent
• Mix for 1 minute, degas for 2 minutes (standard protocol)
• pour onto control layer mold and place mold in vacuum chamber
• Flow layer mixture: 20g Base + 1g Curing agent
• Mix for 1 minute and degas for 2 minutes (standard protocol)
• Spin coat onto flow layer at 2200-2400rpm for 35secs (so far you can use my protocol on a spin coater)
• Remove control layer mold from vacuum chamber, making sure no bubbles are left on the surface (remove with a toothpick if you see some )
• Place the control and flow layer in a 80C convection oven and incubate for 30 minutes (timing is critical here!)
• Remove casts from oven, cut out control layer, punch holes, and align to flow layer
• Put aligned device back into 80C oven and incubate for at least 90 minutes (and here you can increase the backing)
• Remove devices from oven and punch flow layer holes
