Latest revision as of 08:48, 15 July 2011

Glycerol stock

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Material needed: overnight cultures of transformed cells (do not centrifuge them) and glycerol 50%

Mix gently the cell cultures to resuspend the pellet
In an Effendorf tube, put 500 ul of glycerol 50% and 500 ul of cell culture (always keep 1:1 ratio)
Label the tube
Mix gently and put the Eppendrofs quickly in the -80°C freezer (glycerol is toxic for the cells)

Revision as of 08:25, 14 July 2011 (view source) Nguenat (Talk \| contribs) (Created page with "{{:Team:EPF-Lausanne/Templates/Header\|title=Glycerol stock }} Material needed: overnight cultures of transformed cells (do not centrifuge them) and glycerol 50% * Mix gently t...") ← Older edit		Latest revision as of 08:48, 15 July 2011 (view source) Dwatson (Talk \| contribs)
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