Team:Freiburg/Notebook/21 June

From 2011.igem.org

(Difference between revisions)
(→Sequencing)
(→Sequencing)
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Dilution of stock: 2 μl in 100 μl H2O.<br/>
Dilution of stock: 2 μl in 100 μl H2O.<br/>
max 100 ng of DNA.
max 100 ng of DNA.
 +
 +
 +
{| cellpadding="10" cellspacing="0" border="1"
 +
|pos. clone
 +
|ng/μl
 +
|μl
 +
|-
 +
|PR1e
 +
|46.8
 +
|2.1
 +
|-
 +
|PR2d
 +
|67.9
 +
|1.4
 +
|-
 +
|PR3e
 +
|45.8
 +
|2.2
 +
|-
 +
|PR4b
 +
|61.5
 +
|1.6
 +
|-
 +
|PR5a
 +
|74.5
 +
|1.3
 +
|-
 +
|PR6c
 +
|94.7
 +
|1.06
 +
|-
 +
|PR7b
 +
|69.1
 +
|1.4
 +
|-
 +
|PR8b
 +
|81.9
 +
|1.2
 +
|-
 +
|PR9a
 +
|64.4
 +
|1.5
 +
|-
 +
|lys 1
 +
|16
 +
|10
 +
|-
 +
|lys 2
 +
|21.7
 +
|10
 +
|-
 +
|lys 3
 +
|2.5
 +
|10
 +
|-
 +
|lys 4
 +
|16.4
 +
|10
 +
|-
 +
|lys 5
 +
|10.2
 +
|10
 +
|}

Revision as of 08:51, 13 July 2011

16. Labday

Sequencing

Primer concentration has to be 2 nM.
Dilution of stock: 2 μl in 100 μl H2O.
max 100 ng of DNA.


pos. clone ng/μl μl
PR1e 46.8 2.1
PR2d 67.9 1.4
PR3e 45.8 2.2
PR4b 61.5 1.6
PR5a 74.5 1.3
PR6c 94.7 1.06
PR7b 69.1 1.4
PR8b 81.9 1.2
PR9a 64.4 1.5
lys 1 16 10
lys 2 21.7 10
lys 3 2.5 10
lys 4 16.4 10
lys 5 10.2 10
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