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Team:Freiburg/Notebook/18 June
From 2011.igem.org
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(→3A assembly: Ligation) |
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S11: heat sensitive cI QPI with a high promoter preceding the repressor<br/> | S11: heat sensitive cI QPI with a high promoter preceding the repressor<br/> | ||
S15: Bacteriophage Holin Gene pS105<br/> | S15: Bacteriophage Holin Gene pS105<br/> | ||
| - | S1, S2, S3: | + | S1, S2, S3: promotor<br/> |
S4, S5, S6: RBS<br/> | S4, S5, S6: RBS<br/> | ||
Target vector | Target vector | ||
Revision as of 08:12, 13 July 2011
13. Labday
3A assembly: Ligation
Investigators: Theo
Comments: Ligation of digested parts from yesterday.
S11: heat sensitive cI QPI with a high promoter preceding the repressor
S15: Bacteriophage Holin Gene pS105
S1, S2, S3: promotor
S4, S5, S6: RBS
Target vector
Transformation
Investigators: Theo
Transformation of ligated parts. Incubation at 30°C because of the heat sensitive promotor.
