Team:Freiburg/Notebook/18 June

From 2011.igem.org

(Difference between revisions)
(3A assembly: Ligation)
(Transformation)
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'''Investigators: Theo'''
'''Investigators: Theo'''
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Transformation of ligated parts. Incubation at 30°C.
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Transformation of ligated parts. Incubation at 30°C because of the heat sensitive promotor.

Revision as of 08:08, 13 July 2011

13. Labday


3A assembly: Ligation

Investigators: Theo

Comments: Ligation of digested parts from yesterday.

S11: heat sensitive cI QPI with a high promoter preceding the repressor
S15: Bacteriophage Holin Gene pS105
S1-S6
Target vector

Transformation

Investigators: Theo

Transformation of ligated parts. Incubation at 30°C because of the heat sensitive promotor.