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Team:EPF-Lausanne/Protocols/Gel purification
From 2011.igem.org
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| - | Purpose: to purify a band on a gel | + | '''Purpose''': to purify a band on a gel |
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After having verified that the electrophoresis gives you the expected fragments, re-do an electrophoresis while using the thicker combs. You can put up to 25-30 ul in the wells. | After having verified that the electrophoresis gives you the expected fragments, re-do an electrophoresis while using the thicker combs. You can put up to 25-30 ul in the wells. | ||
Revision as of 10:54, 12 July 2011
Gel purification
Purpose: to purify a band on a gel
After having verified that the electrophoresis gives you the expected fragments, re-do an electrophoresis while using the thicker combs. You can put up to 25-30 ul in the wells.
First, cut out the band from the gel
- Take the black box off the UV plate
- Hold the anti-UV shield with one hand and switch on the UV light
- Cut around the band, switch off the UV light and put the removed band in an eppendorf tube
Then, to extract the DNA from the gel, use the Qiagen gel purification kit.
- To weight your samples, tare the scales with an empty tube
- In the elution step, when you have to centrifuge the column in a new Eppendorf tupe, make sure the lid of the tube flies behind in the centrifugator. It turns counterclockwise.
