Revision as of 09:40, 12 July 2011

Gel purification

Purpose: to purify a band on a gel

First, cut out the band from the gel

Take the black box off the UV plate
Hold the anti-UV shield with one hand and switch on the UV light
Cut around the band, switch off the UV light and put the removed band in an eppendorf tube

Then, to extract the DNA from the gel, use the Qiagen gel purification kit.

To weight your samples, tare the scales with an empty tube
In the elution step, when you have to centrifuge the column in a new Eppendorf tupe, make sure the lid of the tube flies behind in the centrifugator. It turns counterclockwise.

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-{{:Team:EPF-Lausanne/Templates/Header|title=Agarose gel electrophoresis}}
+{{:Team:EPF-Lausanne/Templates/Header|title=Gel purification}}
+Purpose: to purify a band on a gel
+First, cut out the band from the gel
+* Take the black box off the UV plate
+* Hold the anti-UV shield with one hand and switch on the UV light
+* Cut around the band, switch off the UV light and put the removed band in an eppendorf tube
+Then, to extract the DNA from the gel, use the Qiagen gel purification kit.
+* To weight your samples, tare the scales with an empty tube
+* In the elution step, when you have to centrifuge the column in a new Eppendorf tupe, make sure the lid of the tube flies behind in the centrifugator. It turns counterclockwise.
 {{:Team:EPF-Lausanne/Templates/Footer}}