Team:Freiburg/Notebook/17 June
From 2011.igem.org
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(→PCR with Ccar and Ccas) |
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|Ccar | |Ccar | ||
|4 and 6 | |4 and 6 | ||
+ | |} | ||
+ | <br/> | ||
+ | <br/> | ||
+ | ==3A assembly: Digestion== | ||
+ | |||
+ | '''Investigators: Theo''' | ||
+ | |||
+ | {| cellpadding="10" cellspacing="1" border="1" | ||
+ | | | ||
+ | |Enzyme 1 | ||
+ | |Enzyme 2 | ||
+ | |– | ||
+ | |Vector | ||
+ | |EcoRI | ||
+ | |PstI | ||
+ | |- | ||
+ | |PartI | ||
+ | |EcoRI | ||
+ | |SpeI | ||
+ | |- | ||
+ | |PartII | ||
+ | |Xbal | ||
+ | |PstI | ||
|} | |} |
Revision as of 12:10, 11 July 2011
12. Labday
PCR with Ccar and Ccas
Investigators: Julia
different primer used for PCR:
Primer | |
Ccas | 5 and 7 |
Ccar | 4 and 6 |
3A assembly: Digestion
Investigators: Theo
Enzyme 1 | Enzyme 2 | – | Vector | EcoRI | PstI | |
PartI | EcoRI | SpeI | ||||
PartII | Xbal | PstI |