Team:Freiburg/Notebook/8 June

From 2011.igem.org

(Difference between revisions)
(Protein modelling and amplification of parts)
(Protein modelling and amplification of parts)
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==Protein modelling and amplification of parts==
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==??.Labday==
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==protein modelling==
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'''Investigators:<br/>''' Protein modelling with pymol: Rüdiger<br/> Amplification of different parts: Julia and Jacob
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'''Investigators: Rüdiger'''<br/>
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==Amplification of different parts==<br/> '''Investigators: Julia and Jacob'''
antibiotics plates:<br/>
antibiotics plates:<br/>
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1oo mikroliter H2O + 40 microliter Tet<br/>
1oo mikroliter H2O + 40 microliter Tet<br/>
1oo mikroliter H2O + 10 microliter Kan<br/>
1oo mikroliter H2O + 10 microliter Kan<br/>
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Revision as of 10:49, 8 July 2011

??.Labday

protein modelling

Investigators: Rüdiger
==Amplification of different parts==
Investigators: Julia and Jacob

antibiotics plates:
1oo mg/ml Ampizilin (100 microliter per plate)
1oo mikroliter H2O + 2 microliter Cm
1oo mikroliter H2O + 40 microliter Tet
1oo mikroliter H2O + 10 microliter Kan


part location(p=plate) resistance info
BBa_K098995 P3, 1E amp heat sensitive cI QPI with high promoter
BBa_K112022 P3, 24E amp Lambda phage lysis device - no promoter, This part is in BBb Format.
It is flanked by BamHI and BglII sites instead of XbaI and SpeI.
pSB1K3 P1, 5A kana
pSB1A3 P1, 1G amp
pSB1C3 P1,3A chloramphenicol
pSB1T3 P1, 7A tet
J23104 P1, 18K
J23110 P1, 20 C
J23116 P1, 20M
B0034 P1, 2M
B0032 P1, 2 I
B0031 P1, 2G