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Team:Wageningen UR/Notebook/Proj2/July
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| - | ''' | + | '''4 July''' |
| - | + | Inoculated 4 Erlenmeyers containing transformation medium with either 0.5*106, 1*106, 5*106 or 107 spores/mL and grew them overnight at 30˚C. This time fresh spores were used which were derived from a spore plate that had grown for 4 days. | |
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| + | '''5 July''' | ||
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| + | Dr. LdG showed me how to recover protoplasts from mycelium. He used the mycelium derived from the 1*106 spores/mL medium, as this one showed the least amount of pellets and decent growth. Myself, Youri and David were assisting him. Buffers with ranging osmotic values were prepared to see whether this had any effect on protoplast formation. This time the protoplast recovery resulted in approximately 80 aliquots. | ||
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| + | '''6 July''' | ||
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| + | Again, a test was carried out to check the amount of viable protoplasts in the protoplast solutions and to check for mycelia contamination. Results will be visible on Friday, 8th July. | ||
[https://2011.igem.org/Team:Wageningen_UR/Notebook/Proj2/June Previous Month] | [https://2011.igem.org/Team:Wageningen_UR/Notebook/Proj2/June Previous Month] | ||
Revision as of 21:30, 7 July 2011
July - Fungal Track 'n Trace
4 July
Inoculated 4 Erlenmeyers containing transformation medium with either 0.5*106, 1*106, 5*106 or 107 spores/mL and grew them overnight at 30˚C. This time fresh spores were used which were derived from a spore plate that had grown for 4 days.
5 July
Dr. LdG showed me how to recover protoplasts from mycelium. He used the mycelium derived from the 1*106 spores/mL medium, as this one showed the least amount of pellets and decent growth. Myself, Youri and David were assisting him. Buffers with ranging osmotic values were prepared to see whether this had any effect on protoplast formation. This time the protoplast recovery resulted in approximately 80 aliquots.
6 July
Again, a test was carried out to check the amount of viable protoplasts in the protoplast solutions and to check for mycelia contamination. Results will be visible on Friday, 8th July.
