Team:Lyon-INSA-ENS/Realisation/Protocols/Microscopy

From 2011.igem.org

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In empty sterile plates, introduce 10mL of M63G medium and 100µL of appropriate antibiotic.<br/>
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<p style="text-align : center"> <font color="green" size="5"> Microscopy test</font> </p>
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This protocol aims at preparing glass slides for observation of biofilms with a fluorescent microscope.
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<p style="text-align : center"> <font color="green" size="5"> Procedure </font> </p>
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<b>1.</b> In empty sterile plates, introduce 10mL of M63G medium and 100µL of appropriate antibiotic.
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<b>2.</b> Add 100µL of bacteria from a saturated liquid culture.
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<b>3.</b> Finally, add 3 or 4 sterile glass slides with a sterile tweezer. 
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Add 100µL of bacteria from a saturated liquid culture. <br/>
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Finally, add 3 or 4 sterile glass slides with a sterile tweezer. <br/>
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<b>4.</b> Incubate at 30°C overnight.
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Incubate at 30°C overnight. <br/><br/>
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<b>5.</b> Observe the slides with a fluorescent microscope.
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Observe the slides with a fluorescent microscope.
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Latest revision as of 10:20, 10 January 2012






Microscopy test





This protocol aims at preparing glass slides for observation of biofilms with a fluorescent microscope.





Procedure



1. In empty sterile plates, introduce 10mL of M63G medium and 100µL of appropriate antibiotic.


2. Add 100µL of bacteria from a saturated liquid culture.


3. Finally, add 3 or 4 sterile glass slides with a sterile tweezer.


4. Incubate at 30°C overnight.


5. Observe the slides with a fluorescent microscope.




























































































































ENS assystem Biomérieux INSA INSA
Retrieved from "http://2011.igem.org/Team:Lyon-INSA-ENS/Realisation/Protocols/Microscopy"