Team:Queens Canada/Side/Reporter

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    <span class="classorange"><a href="#overview"> overview      </a></span>    </regulartext>
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    <span class="classorange"><a href="#glow"> glow worms    </a></span>    </regulartext>
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    <span class="classorange"><a href="#insitu"> <i>in situ </i>    </a></span>    </regulartext>
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    <span class="classorange"><a href="#exsitu"> <i> ex situ </i>    </a></span>    </regulartext>
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<h3orange> Overview </h3orange><p>
<h3orange> Overview </h3orange><p>
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<regulartext> In designing our worm the be the ultimate remediation tool, it is important for us to ask ourselves how we will know where our worms are and when they have finished their job. Thus, we have designed a reporter system using fluorescent protein so that we easily find our worm in soil samples while working in the field. This can make our worm a useful tool by making detection as easy and as simple as possible. </regulartext><br>
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<regulartext> In designing our worm to be the ultimate remediation tool, it is important for to differentiate the worm from wild types. We  designed a reporter system using eCFP (enhanced cyano fluorescent protein) to distinguish the worm. This can make our worm a useful tool by making detection as easy and as simple as possible. </regulartext><p>
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<regulartext>Using the fluorescence of our worm we may also tie the fluorescence into the GPCR detection or nahD degredation pathway. We can then use the worms fluorescence as an approximate measure of how much of our target pollutants are being detected and degraded.</regulartext>
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<regulartext> There are many different applications of fluorescence for bioremediation purposes, both<i> in situ </i> and  <i> ex situ </i>. <p>
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<h3orange>Construct: Fluorescent Worms </h3orange><p>
<h3orange>Construct: Fluorescent Worms </h3orange><p>
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<p><regulartext> This construct demonstrated our neuron specific promoter and fluorescent protein operated as they were designed to. </regulartext>
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<p><regulartext> The next step for fluorescent worms is to use a constitutive promoter for localization to all the body cells. </regulartext>
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Latest revision as of 03:22, 29 October 2011

Overview

In designing our worm to be the ultimate remediation tool, it is important for to differentiate the worm from wild types. We designed a reporter system using eCFP (enhanced cyano fluorescent protein) to distinguish the worm. This can make our worm a useful tool by making detection as easy and as simple as possible.

There are many different applications of fluorescence for bioremediation purposes, both in situ and ex situ .

Construct: Fluorescent Worms

Our team successfully injected C. elegans with a reporter construct. The image below shows the AWB neuron expressing cyan fluorescence.

This construct demonstrated our neuron specific promoter and fluorescent protein operated as they were designed to.

The next step for fluorescent worms is to use a constitutive promoter for localization to all the body cells.

In Situ Applications
Ex Situ Applications

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