Team:Paris Bettencourt/Experiments/Lambda switch

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<h2>Abstract</h2>
<h2>Abstract</h2>
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<div style="margin-left:50px; margin-right:50px; padding: 5px; border:2px solid black;"><b><p>Results for the YFP concentrator:
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<div style="margin-left:50px; margin-right:50px; padding: 5px; border:2px solid black;"><b><p>Results for the Lambda switch design:
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     <li>We successfully BioBricked both the YFP:TetR (<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K606025">BBa_K606025</a>) and the TetO Array (<a href="http://partsregistry.org/Part:BBa_K606026">BBa_K606026</a>) constructs and sent them to the registry</li>
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     <li>We successfully created the cI system (<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K606068">BBa_K606068</a>)</li>
         <li>We integrated our construct both in <i>E.coli</i> and <i>B.subtilis</i></li>
         <li>We integrated our construct both in <i>E.coli</i> and <i>B.subtilis</i></li>
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<li>We characterized the YFP:TetR fusion protein both in <i>E.coli</i> and <i>B.subtilis</i></li>
 
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<li>We characterized the TetO array in <i>E.coli</i></li>
 
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<li>We've done <i>E.coli</i> to <i>B.subtilis</i> diffusion experiments (with negative results)</li>
 
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<h2>Integration into <i>B. subtilis</i></h2>
<h2>Integration into <i>B. subtilis</i></h2>
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[[File:cIbsubtilis.jpg|center|Integration of cI (<partinfo>BBa_K606068</partinfo>)]]
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<center><p>We succeeded to integrate BBa_K606068 into <i>B. subtilis</i> with an episomal vector <partinfo>K090401</partinfo> the 29th of october.</center>
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Latest revision as of 03:21, 29 October 2011

Team IGEM Paris 2011