Team:Grenoble/Projet/Biobricks

From 2011.igem.org

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<div class="body">
<div class="body">
<div class="left">
<div class="left">
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<div class="biobricks">
 
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<h1>Our Biobricks:</h1>
 
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<div class="blocbackground">
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<div class="blocbackground">
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<h1>Biobricks</h1>
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<ul>
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<p>
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<li><a href="http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2011&group=Grenoble">Here</a> you can find all our news and composites parts we send to the registry.</li>
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<a href="http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2011&group=Grenoble">Here</a> you can find all our news and composite parts we send to the registry.
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</p>
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</div class="blocbackground">
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</ul>
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</html>
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<center>
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<groupparts>iGEM011 Grenoble</groupparts>
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</center>
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              <html>
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<div class="blocbackground">
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<ul>
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<li>You can also find in the registry some of our constructions intermediates:</li>
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</ul>
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</p>
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<table>
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<tr>
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<td><a href="http://partsregistry.org/Part:BBa_S04602">BBa_S04602</a></td>
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<td><p><strong>RBS-CinR</strong>, BBa_B0034+BBa_C0077 </p></td>
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</tr>
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<tr>
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<td><a href="http://partsregistry.org/Part:BBa_S04603">BBa_S04603</a></td>
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<td><p><strong>RBS-TetR</strong>, BBa_B0034+BBa_C0040 </p></td>
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</tr> 
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<tr>
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<td><a href="http://partsregistry.org/Part:BBa_S04606">BBa_S04606</a></td>
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<td><p><strong>RBS-TetR-RBS-CinR</strong>, BBa_B0034+BBa_C0040+BBa_B0034+BBa_C0077</p></td>
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</tr>
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<tr>
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<td><a href="http://partsregistry.org/Part:BBa_S04605">BBa_S04605</a></td>
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<td><p><strong>RBS-TetR-RBS-GFP</strong>, BBa_B0034+BBa_C0077+BBa_E0240</p></td>
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</tr>
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<tr>
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<td><a href="http://partsregistry.org/Part:BBa_S04607">BBa_S04607</a></td>
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<td><p><strong>pLac-CinR</strong>, BBa_R0011+BBa_C0077</p></td>
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</tr>
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<tr>
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<td><a href="http://partsregistry.org/Part:BBa_S04618">BBa_S04618</a></td>
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<td><p><strong>pLac-CinR</strong>, BBa_K545006+BBa_I13401</p></td>
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</tr>
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</table>
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</div class="blocbackground">
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</div>
</div>
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                <div class="blocbackground">
 
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                  <table>
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</html>
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<tr>
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<center>
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  <td>
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<groupparts>iGEM011 Grenoble</groupparts>
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      <p align="left">
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</center>
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      MerR-del : K545103.
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<html>
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      MerR (BBa_K346001) without RBS
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      PCR gel
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      Well 1: Positive control (pSB4K5)
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      Well 2: Negative control (Without DNA)
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      Well 3, 4, 5, 6, 7: MerR-del (expected at 740pb, obtain around 700-800pb)
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      Confirmed by sequencing
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      </p>
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  </td>
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  <td>
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    <img src="https://static.igem.org/mediawiki/2011/f/fe/01.png"/>
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  </td>
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</tr>
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<tr>
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<div class="blocbackground">
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  <td>
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<p>
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    <p align="left">
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You can also find in the registry some of our intermediate constructions:
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    pMerT-GFP: K545002
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</p>
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    Original parts: K346002, E0240
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    PCR gel
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<table>
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    Well 1 and 2: Failed cloning
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<tr>
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    Well 3: pMerT-GFP (expected at1200pb obtain just over 1000pb)
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<td><a href="http://partsregistry.org/Part:BBa_S04602">BBa_S04602</a></td>
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    Confirmed by sequencing
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<td><p><strong>RBS-CinR</strong>, BBa_B0034+BBa_C0077 </p></td>
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    </p>
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</tr>
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  </td>
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<tr>
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  <td>
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<td><a href="http://partsregistry.org/Part:BBa_S04603">BBa_S04603</a></td>
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<img src="https://static.igem.org/mediawiki/2011/d/d9/02.png"/>
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<td><p><strong>RBS-TetR</strong>, BBa_B0034+BBa_C0040 </p></td>
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  </td>
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</tr> 
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</tr>
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<tr>
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<td><a href="http://partsregistry.org/Part:BBa_S04606">BBa_S04606</a></td>
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<td><p><strong>RBS-TetR-RBS-CinR</strong>, BBa_B0034+BBa_C0040+BBa_B0034+BBa_C0077</p></td>
 +
</tr>
 +
<tr>
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<td><a href="http://partsregistry.org/Part:BBa_S04605">BBa_S04605</a></td>
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<td><p><strong>RBS-TetR-RBS-GFP</strong>, BBa_B0034+BBa_C0077+BBa_E0240</p></td>
 +
</tr>
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<tr>
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<td><a href="http://partsregistry.org/Part:BBa_S04607">BBa_S04607</a></td>
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<td><p><strong>pLac-CinR</strong>, BBa_R0011+BBa_C0077</p></td>
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</tr>
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<tr>
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<td><a href="http://partsregistry.org/Part:BBa_S04618">BBa_S04618</a></td>
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<td><p><strong>pLac-CinR</strong>, BBa_K545006+BBa_I13401</p></td>
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</tr>  
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</table>
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</div>
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<tr>
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<div class="blocbackground">
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  <td>
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<table align="center">
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    <p align="left">
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<tr>
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    pCin-CrtEBI (K545000)
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<td>
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    Originally: R0077, K274100
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<p align="left">
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    PCR gel
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<center><h5>MerR-del (K545103)</h5></center><br/><br/>
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    Expected at 3500pb, obtain between 3000 and 4000pb
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    Confirmed by sequencing
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MerR (BBa_K346001) without RBS<br/>
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  </td>
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PCR gel<br/><br/>
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  <td>
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<img src="https://static.igem.org/mediawiki/2011/9/9a/03.png"/>
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Well 1: Positive control (pSB4K5)<br/>
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  </td>
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Well 2: Negative control (Without DNA)<br/>
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</tr>
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Wells 3, 4, 5, 6, 7: MerR-del (band expected at 740pb, obtained around 700-800pb)<br/><br/>
 +
 +
Confirmed by sequencing<br/>
 +
</p>
 +
</td>
 +
<td>
 +
<center><img src="https://static.igem.org/mediawiki/2011/f/fe/01.png"/></center>
 +
</td>
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</tr>
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<tr>
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<td>
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<p align="left">
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<center><h5>pMerT-GFP (K545002)</h5></center><br/><br/>
 +
 +
Original parts: K346002, E0240<br/>
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PCR gel<br/><br/>
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 +
Well 1: MW<br/>
 +
Well 2: empty well<br/>
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Well 3 and 4: clonning failure<br/>
 +
Well 5: pMerT-GFP (expected at 1200pb obtained right above 1000pb)<br/><br/>
 +
 +
Confirmed by sequencing<br/>
 +
</p>
 +
</td>
 +
<td>
 +
<center><img src="https://static.igem.org/mediawiki/2011/d/d9/02.png"/></center>
 +
</td>
 +
</tr>
 +
<tr>
 +
<td>
 +
<p align="left">
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<center><h5>pCin-CrtEBI (K545000)</h5></center><br/><br/>
 +
 +
Original parts: R0077, K274100<br/>
 +
PCR gel<br/><br/>
 +
 +
Wells 1, 2 and 3: pCin-CrtEBI (Expected at 3500pb, obtained between 3000 and 4000pb)<br/>
 +
Well 4: MW<br/><br/>
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 +
Confirmed by sequencing<br/>
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</p>
 +
</td>
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<td>
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<center><img src="https://static.igem.org/mediawiki/2011/9/9a/03.png"/></center>
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</td>
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</tr>
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<tr>
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<td>
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<p align="left">
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<center><h5>pConst-RBS-CinR (K545004)</h5></center><br/><br/>
 +
 +
Original parts:  J23119, B0034 C0077<br/>
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Restriction gel<br/><br/>
 +
 +
Wells 1 and 2: pConst-CinR(expected at 950pb)<br/>
 +
Wells 4 and 5: cloning failure<br/>
 +
Wells 3 and 6: empty wells<br/>
 +
plasmids were obtained for each well at the expected size,about 2000pb.<br/><br/>
 +
 +
Confirmed by sequencing<br/>
 +
</p>
 +
</td>
 +
<td>
 +
<center><img src="https://static.igem.org/mediawiki/2011/9/96/04.png"/></center>
 +
</td>
 +
</tr>
 +
<tr>
 +
<td>
 +
<p align="left">
 +
<center><h5>pLac-CinR (S04607)</h5></center><br/><br/>
 +
 +
Original parts: R0011, E0240<br/>
 +
Restriction gel<br/><br/>
 +
 +
<center><h5>pConst-GFP (K545001)</h5></center><br/><br/>
 +
 +
Original parts: J23119, E0240<br/>
 +
Restriction gel<br/><br/>
 +
 +
Well 1: pLac-CinR (expected at 950pb)<br/>
 +
Well 2: pConst-CinR (expected at 900pb)<br/>
 +
plasmids were obtained for each well at the expected size,about 2000pb.<br/><br/>
 +
 +
Confirmed by sequencing<br/>
 +
</p>
 +
</td>
 +
<td>
 +
<center><img src="https://static.igem.org/mediawiki/2011/7/79/05.png"/></center>
 +
</td>
 +
</tr>
 +
<tr>
 +
<td>
 +
<p align="left">
 +
<center><h5>RBS-CinR (S04602)</h5></center><br/><br/>
 +
 +
Original parts: B0034, C0077<br/>
 +
PCR gel<br/><br/>
 +
 +
Well 1: RBS-CinR (expected at 1000)<br/>
 +
Wells 2, 3 and 4: clonning failure<br/><br/>
 +
 +
Confirmed by sequencing<br/>
 +
</p>
 +
</td>
 +
<td>
 +
<center><img src="https://static.igem.org/mediawiki/2011/5/5f/06.png"/></center>
 +
</td>
 +
</tr>
 +
<tr>
 +
<td>
 +
<p align="left">
 +
<center><h5>RBS-TetR (S04603)</h5></center><br/><br/>
 +
 +
Original parts: B0034, C0040<br/>
 +
PCR gel<br/><br/>
 +
 +
well 1: RBS-TetR (expected at 950pb)<br/><br/>
 +
 +
Confirmed by sequencing<br/>
 +
</p>
 +
</td>
 +
<td>
 +
<center><img src="https://static.igem.org/mediawiki/2011/a/af/07.png"/></center>
 +
</td>
 +
</tr>
 +
</table>
 +
</div>
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<tr>
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</div>
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  <td>
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    <p align="left">
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    pConst-RBS-CinR (K545004)
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    Originally J23119, B0034 C0077
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    Restriction gel
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    Well 1, 2: failed cloning
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    Well 3, 4: pConst-CinR(expected at 950pb)
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    Confirmed by sequencing
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-
  </td>
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  <td>
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<img src="https://static.igem.org/mediawiki/2011/9/96/04.png"/>
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-
  </td>
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</tr>
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<tr>
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</div>
-
  <td>
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    <p align="left">
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    pLac-CinR (S04607)
+
-
    Originally R0011, E0240
+
-
    pConst-GFP (K545001)
+
-
    Originally J23119, E0240
+
-
    Restriction gel
+
-
    Well 1: pLac-CinR (expected at 950pb)
+
-
    Well 2: pConst-CinR (expected at 900pb)
+
-
    Confirmed by sequencing
+
-
  </td>
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  <td>
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<img src="https://static.igem.org/mediawiki/2011/7/79/05.png"/>
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-
  </td>
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</tr>
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<tr>
 
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  <td>
 
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    <p align="left">
 
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    RBS-CinR (S04602)
 
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    Originally B0034, C0077
 
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    PCR gel
 
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    Well 1: RBS-CinR (expected at 1000)
 
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    Confirmed by sequencing
 
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  </td>
 
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  <td>
 
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<img src="https://static.igem.org/mediawiki/2011/5/5f/06.png"/>
 
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  </td>
 
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</tr>
 
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<tr>
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</html>
-
  <td>
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-
    <p align="left">
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-
    RBS-TetR (S04603)
+
-
    Originally B0034, C0040
+
-
    PCR gel
+
-
    RBS-TetR (expected at 950pb)
+
-
    Confirmed by sequencing
+
-
  </td>
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  <td>
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-
<img src="https://static.igem.org/mediawiki/2011/a/af/07.png"/>
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  </td>
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</tr>
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</table>
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{{:Team:Grenoble/Design/pied}}
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Latest revision as of 00:05, 29 October 2011

Grenoble 2011, Mercuro-Coli iGEM


Biobricks

Here you can find all our news and composite parts we send to the registry.

<groupparts>iGEM011 Grenoble</groupparts>

You can also find in the registry some of our intermediate constructions:

BBa_S04602

RBS-CinR, BBa_B0034+BBa_C0077

BBa_S04603

RBS-TetR, BBa_B0034+BBa_C0040

BBa_S04606

RBS-TetR-RBS-CinR, BBa_B0034+BBa_C0040+BBa_B0034+BBa_C0077
BBa_S04605

RBS-TetR-RBS-GFP, BBa_B0034+BBa_C0077+BBa_E0240
BBa_S04607

pLac-CinR, BBa_R0011+BBa_C0077
BBa_S04618

pLac-CinR, BBa_K545006+BBa_I13401

MerR-del (K545103)


MerR (BBa_K346001) without RBS
PCR gel

Well 1: Positive control (pSB4K5)
Well 2: Negative control (Without DNA)
Wells 3, 4, 5, 6, 7: MerR-del (band expected at 740pb, obtained around 700-800pb)

Confirmed by sequencing

pMerT-GFP (K545002)


Original parts: K346002, E0240
PCR gel

Well 1: MW
Well 2: empty well
Well 3 and 4: clonning failure
Well 5: pMerT-GFP (expected at 1200pb obtained right above 1000pb)

Confirmed by sequencing

pCin-CrtEBI (K545000)


Original parts: R0077, K274100
PCR gel

Wells 1, 2 and 3: pCin-CrtEBI (Expected at 3500pb, obtained between 3000 and 4000pb)
Well 4: MW

Confirmed by sequencing

pConst-RBS-CinR (K545004)


Original parts: J23119, B0034 C0077
Restriction gel

Wells 1 and 2: pConst-CinR(expected at 950pb)
Wells 4 and 5: cloning failure
Wells 3 and 6: empty wells
plasmids were obtained for each well at the expected size,about 2000pb.

Confirmed by sequencing

pLac-CinR (S04607)


Original parts: R0011, E0240
Restriction gel

pConst-GFP (K545001)


Original parts: J23119, E0240
Restriction gel

Well 1: pLac-CinR (expected at 950pb)
Well 2: pConst-CinR (expected at 900pb)
plasmids were obtained for each well at the expected size,about 2000pb.

Confirmed by sequencing

RBS-CinR (S04602)


Original parts: B0034, C0077
PCR gel

Well 1: RBS-CinR (expected at 1000)
Wells 2, 3 and 4: clonning failure

Confirmed by sequencing

RBS-TetR (S04603)


Original parts: B0034, C0040
PCR gel

well 1: RBS-TetR (expected at 950pb)

Confirmed by sequencing

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