Team:NYMU-Taipei/results/chassis1

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Revision as of 21:59, 28 October 2011 (view source) Nymuchang (Talk | contribs) (→Growth Curve Of AMB-1 (BBa_K624032)) ← Older edit		Revision as of 21:59, 28 October 2011 (view source) Nymuchang (Talk | contribs) Newer edit →
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	<font size=3>We measure AMB-1's growth under OD565, which is the best absorption wavelength for AMB-1. For electroporation operation, it is important to find out the log phase, since it’s the best time for electroporation.		<font size=3>We measure AMB-1's growth under OD565, which is the best absorption wavelength for AMB-1. For electroporation operation, it is important to find out the log phase, since it’s the best time for electroporation.
	By keeping AMB-1 in "log phase" (approximately <font color=blue>'''3~6 days'''</font> in Enrich-MSGM, <font color=blue>'''7~10 days'''</font> in MSGM), best results of electroporation are achieved. And after seven days, the bacterium would go into '''stationary phase'''. During the phase, the growth slows as a result of nutrient depletion and accumulation of toxic products. Growth rate and death rate of bacterium is best likely to meet equilibrium in this stage. </font>		By keeping AMB-1 in "log phase" (approximately <font color=blue>'''3~6 days'''</font> in Enrich-MSGM, <font color=blue>'''7~10 days'''</font> in MSGM), best results of electroporation are achieved. And after seven days, the bacterium would go into '''stationary phase'''. During the phase, the growth slows as a result of nutrient depletion and accumulation of toxic products. Growth rate and death rate of bacterium is best likely to meet equilibrium in this stage. </font>
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	=='''<font size=5><font color=crimson>pYMB Test Result</font></font>'''==		=='''<font size=5><font color=crimson>pYMB Test Result</font></font>'''==

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Revision as of 21:59, 28 October 2011

• Home Get to HOME
• Idea Our Idea
  Concept Brainstorming Background Review
• Design/ Experiment
  Optomagnetic Design Protein Modelling NeuroSymbiosis Chassis SynthoPrime Lab Protocol
• Results
  Optomagnetic Bacteria NeuroSymbiosis Chassis Parts International Collaboration
• Human Practice & Safety
  Synthetic Neurobiology Human Practice Evaluation & Safety Assessment
• About Us
  The School The Team China Meetup Acknowledgement

"Magnetotaxis": Chassis Page

Chassis page titled "[http://partsregistry.org/Magnetotaxis Magnetotaxis]" has been created in Parts Registry, please do visit this site for more useful information related to this species and the newly established chassis.

Growth Curve Of AMB-1 (BBa_K624032)

AMB-1 growth curve with MSGM.
The Horizontal axis is the time of growth (in days) and the Vertical axis is the OD565 (the maximum absorbance of AMB-1). The AMB-1 enters log phase after about 7~10 days.

We measure AMB-1's growth under OD565, which is the best absorption wavelength for AMB-1. For electroporation operation, it is important to find out the log phase, since it’s the best time for electroporation. By keeping AMB-1 in "log phase" (approximately 3~6 days in Enrich-MSGM, 7~10 days in MSGM), best results of electroporation are achieved. And after seven days, the bacterium would go into stationary phase. During the phase, the growth slows as a result of nutrient depletion and accumulation of toxic products. Growth rate and death rate of bacterium is best likely to meet equilibrium in this stage.

pYMB Test Result

International Collaboration Reports

One of our collaborators, Tsinghua has claimed pYMBG ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K624021 BBa_K624021]) as an effective shuttle vector.