Team:NYMU-Taipei/results/chassis1
From 2011.igem.org
(→Growth Curve Of AMB-1 (BBa_K624032)) |
|||
Line 21: | Line 21: | ||
[[Image:AMB-1growthcurve.PNG|frame|center|'''AMB-1 growth curve with MSGM.''' <br> | [[Image:AMB-1growthcurve.PNG|frame|center|'''AMB-1 growth curve with MSGM.''' <br> | ||
The Horizontal axis is the time of growth (in days) and the Vertical axis is the OD565 (the maximum absorbance of AMB-1). The AMB-1 enters log phase after about 7~10 days.]] | The Horizontal axis is the time of growth (in days) and the Vertical axis is the OD565 (the maximum absorbance of AMB-1). The AMB-1 enters log phase after about 7~10 days.]] | ||
+ | |||
<font size=3>We measure AMB-1's growth under OD565, which is the best absorption wavelength for AMB-1. For electroporation operation, it is important to find out the log phase, since it’s the best time for electroporation. | <font size=3>We measure AMB-1's growth under OD565, which is the best absorption wavelength for AMB-1. For electroporation operation, it is important to find out the log phase, since it’s the best time for electroporation. | ||
By keeping AMB-1 in "log phase" (approximately <font color=blue>'''3~6 days'''</font> in Enrich-MSGM, <font color=blue>'''7~10 days'''</font> in MSGM), best results of electroporation are achieved. And after seven days, the bacterium would go into '''stationary phase'''. During the phase, the growth slows as a result of nutrient depletion and accumulation of toxic products. Growth rate and death rate of bacterium is best likely to meet equilibrium in this stage. </font> | By keeping AMB-1 in "log phase" (approximately <font color=blue>'''3~6 days'''</font> in Enrich-MSGM, <font color=blue>'''7~10 days'''</font> in MSGM), best results of electroporation are achieved. And after seven days, the bacterium would go into '''stationary phase'''. During the phase, the growth slows as a result of nutrient depletion and accumulation of toxic products. Growth rate and death rate of bacterium is best likely to meet equilibrium in this stage. </font> | ||
- | |||
=='''<font size=5><font color=crimson>pYMB Test Result</font></font>'''== | =='''<font size=5><font color=crimson>pYMB Test Result</font></font>'''== |
Revision as of 21:59, 28 October 2011
![](https://static.igem.org/mediawiki/2011/0/0a/Header_final.png)
Contents |
"Magnetotaxis": Chassis Page
Chassis page titled "[http://partsregistry.org/Magnetotaxis Magnetotaxis]" has been created in Parts Registry, please do visit this site for more useful information related to this species and the newly established chassis.
Growth Curve Of AMB-1 (BBa_K624032)
We measure AMB-1's growth under OD565, which is the best absorption wavelength for AMB-1. For electroporation operation, it is important to find out the log phase, since it’s the best time for electroporation.
By keeping AMB-1 in "log phase" (approximately 3~6 days in Enrich-MSGM, 7~10 days in MSGM), best results of electroporation are achieved. And after seven days, the bacterium would go into stationary phase. During the phase, the growth slows as a result of nutrient depletion and accumulation of toxic products. Growth rate and death rate of bacterium is best likely to meet equilibrium in this stage.
pYMB Test Result
International Collaboration Reports
One of our collaborators, Tsinghua has claimed pYMBG ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K624021 BBa_K624021]) as an effective shuttle vector.