User:Allancrossman

From 2011.igem.org

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Allan Crossman, member of [[Team:Edinburgh|Edinburgh 2011]].
 
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==Personal TODO list==
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<p class="h1">Allan Crossman</p>
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* What happened to our primers e.g. for xylA?
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Allan Crossman, member of [[Team:Edinburgh|Edinburgh 2011]].
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* What is density of displayed INP?
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* Plan for zipper system.
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* Plan for pIII beads reactor?
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* Can we suppress the hover-over thing for pages with no hard words?
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* The hover-over thing fails on IE7?
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* Add hints.
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==Notes to self==
==Notes to self==
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* We MUST have (somewhere) a team description page, a project abstract, a complete project description, a lab notebook, and a safety page.
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* [[Judging]]
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* ''inaZ'' is [http://www.ncbi.nlm.nih.gov/nuccore/71553748?from=1850733&to=1855854 here], broken by an IS.
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* [https://igem.org/Results?year=2011 2011 full results]
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* To add hints, edit [[Team:Edinburgh/tech/Hints]] and also add &lt;span class="hardword" id="foo"&gt;foo&lt;/span&gt; in the relevant page.
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* [http://mic.sgmjournals.org/content/142/7/1659.full.pdf+html bglX info]
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* Assembly page is here: [[Team:Edinburgh/AssemblyContent]]
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* [[User:Allancrossman/Gibson]] and [[User:Allancrossman/Gibson2]]
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* The bglX part has a PstI site in it. Once fixed, the official part docs will need to be redone.
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* [http://www.ncbi.nlm.nih.gov/nuccore/AF130864.1 pG8SAET]
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* [http://www.lifesci.dundee.ac.uk/groups/tracy_palmer/docs/signals.htm Substrates of TAT pathway in ''E. coli'']
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* [[Special:Watchlist]]
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==Video game concept==
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You control a humble ribosome. You are powered by sugar. Outside the cell is cellobiose floating around. You can express B-glucosidase on the cell exterior to get more sugar. You can express other stuff. There's cellulose floating around too. You can express cellulases. There's phage. You can express proteases or somesuch?
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==Recombination avoidance test?==
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If INP works, and if we have the money, we could test whether our recombination avoidance strategy actually works, by getting a new version of BBa_K265008 synthesised with synonymous codons, and setting up a construct such that some reporter (e.g. LacZ, YFP, or whatever) would only be expressed if recombination occurred:
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: Promoter - RBS - INP1 - Terminator - Space - INP2 - Reporter
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See if there is any difference between this and:
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: Promoter - RBS - INP1 - Terminator - Space - INP1 - Reporter
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...when cloned into a RecA positive ''E. coli'' strain.
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==Lignin degradation==
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* Requires glucose oxidase to supply H2O2, a cofactor for other enzymes...
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==Stuff others are doing==
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** Lignin peroxidase
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** Manganaese peroxidase
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** Laccase?
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Some of these use flavin cofactors but it seems proteins using these can be successfully displayed using INP (Van Bloois ''et al'', 2009).
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* [https://2011.igem.org/Special:Contributions/Yazbo91 Contributions: Yassen]
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* [https://2011.igem.org/Special:Contributions/L.Kopec Contributions: Lukasz]
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* [https://2011.igem.org/Special:Contributions/mclee Contributions: Lee]
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* [https://2011.igem.org/Special:Contributions/sylvia.ispasani Contributions: Sylvia]
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* [https://2011.igem.org/Special:Contributions/Fionntom Contributions: Fionn]
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* [https://2011.igem.org/Special:Contributions/Di.L Contributions: Di]
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==References==
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==Competitors in our track==
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* Van Bloois E, Winter RT, Janssen DB, Fraaije MW (2009) [http://www.springerlink.com/content/d471504054865565/fulltext.pdf Export of functional ''Streptomyces coelicolor'' alditol oxidase to the periplasm or cell surface of ''Escherichia coli'' and its application in whole-cell biocatalysis]. ''Applied Microbiology and Biotechnology'' '''83''': 679-687 (doi: 10.1007/s00253-009-1904-0).
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: '''Progress to MIT:'''
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:: [[Team:Edinburgh]] (gold)
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:: [[Team:Johns_Hopkins]] (bronze)
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:: [[Team:UTP-Panama]] (bronze)
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:: [[Team:Washington]] (gold, Americas overall winner)
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:: [[Team:Yale]] (gold, Americas finalist)
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: '''Did not progress:'''
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:: [[Team:Alberta]]
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:: [[Team:Debrecen_Hungary]]
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:: [[Team:HIT-Harbin]]
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:: [[Team:Korea_U_Seoul]]
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:: [[Team:Nevada]]
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:: [[Team:Tec-Monterrey]]
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:: [[Team:Tianjin]]
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:: [[Team:UNAM-Genomics_Mexico]]
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:: [[Team:UST-Beijing]]
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:: [[Team:WashU]]
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Latest revision as of 18:38, 28 October 2011

Allan Crossman

Allan Crossman, member of Edinburgh 2011.

Notes to self

Stuff others are doing

Competitors in our track

Progress to MIT:
Team:Edinburgh (gold)
Team:Johns_Hopkins (bronze)
Team:UTP-Panama (bronze)
Team:Washington (gold, Americas overall winner)
Team:Yale (gold, Americas finalist)
Did not progress:
Team:Alberta
Team:Debrecen_Hungary
Team:HIT-Harbin
Team:Korea_U_Seoul
Team:Nevada
Team:Tec-Monterrey
Team:Tianjin
Team:UNAM-Genomics_Mexico
Team:UST-Beijing
Team:WashU

Retrieved from "http://2011.igem.org/User:Allancrossman"