User:Allancrossman

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Allan Crossman, member of [[Team:Edinburgh|Edinburgh 2011]].
Allan Crossman, member of [[Team:Edinburgh|Edinburgh 2011]].
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==Personal TODO list==
 
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* Get INP-YFP localisation info from Sylvia.
 
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* Get those pics from Eugene.
 
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* Work out complete sequence of the PlacLacZ-INP we have and prepare for Gibson using it.
 
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* RFC for BioSandwich
 
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* Table of all our stuff, whether it has RBS, start codon, stop codon, etc.
 
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* Add hints.
 
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* Plan for pIII beads reactor?
 
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* Can we suppress the hover-over thing for pages with no hard words?
 
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* [[Team:Edinburgh/Carotenoids]]
 
==Notes to self==
==Notes to self==
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* [[Judging]]
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* [https://igem.org/Results?year=2011 2011 full results]
* [http://mic.sgmjournals.org/content/142/7/1659.full.pdf+html bglX info]
* [http://mic.sgmjournals.org/content/142/7/1659.full.pdf+html bglX info]
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* We must have a "[https://igem.org/Sample_Data_Page data page]" that explains the system and links to actual data which '''must be on the Registry'''.
 
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* We must have (somewhere) a team description page, a project abstract, a complete project description, a lab notebook, and a safety page.
 
* [[User:Allancrossman/Gibson]] and [[User:Allancrossman/Gibson2]]
* [[User:Allancrossman/Gibson]] and [[User:Allancrossman/Gibson2]]
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* ''inaZ'' is [http://www.ncbi.nlm.nih.gov/nuccore/71553748?from=1850733&to=1855854 here], broken by an IS.
 
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* [http://www.lifesci.dundee.ac.uk/groups/tracy_palmer/docs/signals.htm Substrates of TAT pathway in ''E. coli'']
 
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* [https://2010.igem.org/Team:Warsaw/Stage1/RBSMeas RBS strengths from Warsaw 2010]
 
* [http://www.ncbi.nlm.nih.gov/nuccore/AF130864.1 pG8SAET]
* [http://www.ncbi.nlm.nih.gov/nuccore/AF130864.1 pG8SAET]
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* [[Special:Watchlist]]
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 +
==Stuff others are doing==
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* [https://2011.igem.org/Special:Contributions/Yazbo91 Contributions: Yassen]
* [https://2011.igem.org/Special:Contributions/Yazbo91 Contributions: Yassen]
* [https://2011.igem.org/Special:Contributions/L.Kopec Contributions: Lukasz]
* [https://2011.igem.org/Special:Contributions/L.Kopec Contributions: Lukasz]
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==Large display of small GIF==
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* [https://2011.igem.org/Special:Contributions/mclee Contributions: Lee]
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* [https://2011.igem.org/Special:Contributions/sylvia.ispasani Contributions: Sylvia]
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<html>
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* [https://2011.igem.org/Special:Contributions/Fionntom Contributions: Fionn]
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<div class="center"><div class="thumb tnone"><div class="thumbinner" style="width:622px;"><a href="/File:Syn-Variants.png" class="image"><img alt="" src="/wiki/images/d/df/Syn.gif" width="620" height="310" class="thumbimage" /></a>  <div class="thumbcaption"><div class="magnify"><a href="/File:Syn.gif" class="internal" title="Enlarge"><img src="/wiki/skins/common/images/magnify-clip.png" width="15" height="11" alt="" /></a></div>Non-synergistic versus synergistic systems. Dark regions are places where the cellulose has been degraded down to free glucose molecules.</div></div></div></div>
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* [https://2011.igem.org/Special:Contributions/Di.L Contributions: Di]
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</html>
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==Video game concept==
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You control a humble ribosome. You are powered by sugar. Outside the cell is cellobiose floating around. You can express B-glucosidase on the cell exterior to get more sugar. You can express other stuff. There's cellulose floating around too. You can express cellulases. There's phage. You can express proteases or somesuch?
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==Lignin degradation==
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* Requires glucose oxidase to supply H2O2, a cofactor for other enzymes...
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** Lignin peroxidase
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** Manganaese peroxidase
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** Laccase?
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Some of these use flavin cofactors but it seems proteins using these can be successfully displayed using INP (Van Bloois ''et al'', 2009).
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==Gold medal criteria==
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* Our wiki watch page is clearly a type of collaboration.
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* Cooperation with Trieste re: ''C. fimi'' BGlu sequence.
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* Cooperation with Trieste re: ''C. fimi'' BGlu assay.
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* Registry update for K415151.
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* Registry update for K392008.
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* Registry update for K265008.
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* All our human practices stuff.
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==Tables for Yassen==
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{| class="wikitable centredtext"
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|-
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!Equipment
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!Size (m<sup>3</sup>)
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!Cost constant (&pound;)
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!Index
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!Purchase cost (&pound;)
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!Number required
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!Total cost (&pound;)
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|-
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|Boiler
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|8
+
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|70
+
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|0.8
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|13,218.33
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|4
+
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|52,873.31
+
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|-
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|Reactor 1
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|4
+
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|18,5000
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|0.45
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|47,158.74
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|1
+
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|47,158.74
+
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|-
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|Reactor 2
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|4
+
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|18,500
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|0.45
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|34,522.22
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|1
+
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|34,522.22
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|-
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|Reactor 3
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|4
+
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|18,500
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|0.45
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|34,522.22
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|1
+
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|34,522.22
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|-
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|Reactor 4
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|4
+
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|18,500
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|0.45
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|34,522.22
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|1
+
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|34,522.22
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|-
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|Reactor 5
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|4
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|18,500
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|0.45
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|34,522.22
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|1
+
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|34,522.22
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|-
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|Reactor 6
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|4
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|18,500
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|0.45
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|34,522.22
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|1
+
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|34,522.22
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|-
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|Tank 100-102
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|3.3
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|1,400
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|0.55
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|2,699.67
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|3
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|8099.01
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|-
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|Tank 103
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|93.2
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|1,400
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|0.55
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|16,955.35
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|1
+
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|16,955.35
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|-
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|Tank 104
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|40
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|1,400
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|0.55
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|10,647.83
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|1
+
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|10,647.83
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|-
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|Tank 105
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|147
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|1,400
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|0.55
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|21,784.75
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|1
+
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|21,784.75
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|-
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|Tank 106
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|17
+
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|1,400
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|0.55
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|6,650.82
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|1
+
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|6,650.82
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|}
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{| class="wikitable centredtext"
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|-
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!Factorial number
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!Item
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!Factor
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|-
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|f1
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|Equipment erection
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|0.45
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|-
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|f2
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|Piping
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|0.45
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|-
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|f3
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|Instrumentation
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|0.15
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|-
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|f4
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|Electrical
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|0.1
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|-
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|f5
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|Buildings, process
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|0.1
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|-
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|f6
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|Ancillary buildings
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|0.2
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|-
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|colspan="2"
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|'''Total = 1.45'''
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|-
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|f10
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|Design and Engineering
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|0.25
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|-
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|f11
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|Contractor’s Fee
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|0.05
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|-
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|f12
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|Contingency
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|0.1
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|-
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|colspan="2"
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|'''Total = 0.4'''
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|}
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{| class="wikitable centredtext"
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|-
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!Type
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!Amount
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!Unit price
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!Cost
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|-
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|Steam
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|200 kg/h
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|&pound;7/t
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|&pound;9811.2
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|-
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|Cooling water
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|3,000 kg/h
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|1.5p/t
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|&pound;315.36
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|-
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|Power
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|100 kwh/d
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|1.2p/mj
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|&pound;43,200
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|}
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{| class="wikitable centredtext"
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|-
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!Equipment number
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!Equipment name
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!Comment
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|-
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|Tank 100,101,102
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|Feed storage tanks
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|Capacity: 3.3 m<sup>3</sup>
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|-
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|Tank 103
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|Product storage tank
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|Capacity: 93.2 m<sup>3</sup>
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|-
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|Tank 104
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|Product storage tank
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|Capacity: 40 m<sup>3</sup>
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|-
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|Tank 105
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|Product storage tank
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|Capacity: 147 m<sup>3</sup>
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|-
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|Tank 106
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|Product storage tank
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|Capacity: 17 m<sup>3</sup>
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|-
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|colspan="3"|&nbsp;
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|-
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|R-1
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|Pre-treatment reactor
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|Capacity:8 m<sup>3, 140 C, 20 bar
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|-
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|R-2
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|Thermal depolymerisation of hemicellulose
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|Capacity: 4 m<sup>3</sup>
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|-
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|R-3
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|Hydrous pyrolysis of lignin
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|Capacity: 4 m<sup>3</sup>
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|-
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|R-4
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|Degradation of cellulose
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|Capacity: 4 m<sup>3</sup>
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|-
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|R-5
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|Conversion of glucose to sorbitol
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|Capacity: 4 m<sup>3</sup>
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|-
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|R-6
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|Conversion of glucose to fructose syrup
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|Capacity 4 m<sup>3</sup>
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|-
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|P-1, P-2
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|Pumps
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|
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|-
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|D-1
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|Recovery column
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|1 bar
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|-
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|HE-1, 2, 3, 4
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|Heat exchangers
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|
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|-
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|LI-1, 2, 3, 4, 5, 6, 7, 8, 9
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|Level indicators
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|PID control
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|-
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|TI-1, 2, 3, 4, 5, 6
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|Temperature indicators
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|PID control
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|-
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|FI-1 to FI-15
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|Flow indicators
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|PID control
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|-
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|V-1, 2, 3, 6, 7, 8
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|Mixing valve
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|
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|-
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|V-4, 5
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|Angle valve
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|
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|-
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|V-9 to V-42
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|Gate valve
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|
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|}
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==References==
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==Competitors in our track==
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* Van Bloois E, Winter RT, Janssen DB, Fraaije MW (2009) [http://www.springerlink.com/content/d471504054865565/fulltext.pdf Export of functional ''Streptomyces coelicolor'' alditol oxidase to the periplasm or cell surface of ''Escherichia coli'' and its application in whole-cell biocatalysis]. ''Applied Microbiology and Biotechnology'' '''83''': 679-687 (doi: 10.1007/s00253-009-1904-0).
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: '''Progress to MIT:'''
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:: [[Team:Edinburgh]] (gold)
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:: [[Team:Johns_Hopkins]] (bronze)
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:: [[Team:UTP-Panama]] (bronze)
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:: [[Team:Washington]] (gold, Americas overall winner)
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:: [[Team:Yale]] (gold, Americas finalist)
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: '''Did not progress:'''
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:: [[Team:Alberta]]
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:: [[Team:Debrecen_Hungary]]
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:: [[Team:HIT-Harbin]]
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:: [[Team:Korea_U_Seoul]]
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:: [[Team:Nevada]]
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:: [[Team:Tec-Monterrey]]
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:: [[Team:Tianjin]]
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:: [[Team:UNAM-Genomics_Mexico]]
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:: [[Team:UST-Beijing]]
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:: [[Team:WashU]]
</div> <!-- /main_body-->
</div> <!-- /main_body-->
<html></div> <!-- /mids --></html>
<html></div> <!-- /mids --></html>

Latest revision as of 18:38, 28 October 2011

Allan Crossman

Allan Crossman, member of Edinburgh 2011.

Notes to self

Stuff others are doing

Competitors in our track

Progress to MIT:
Team:Edinburgh (gold)
Team:Johns_Hopkins (bronze)
Team:UTP-Panama (bronze)
Team:Washington (gold, Americas overall winner)
Team:Yale (gold, Americas finalist)
Did not progress:
Team:Alberta
Team:Debrecen_Hungary
Team:HIT-Harbin
Team:Korea_U_Seoul
Team:Nevada
Team:Tec-Monterrey
Team:Tianjin
Team:UNAM-Genomics_Mexico
Team:UST-Beijing
Team:WashU

Retrieved from "http://2011.igem.org/User:Allancrossman"