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Team:UPO-Sevilla/Foundational Advances/Biobrick Creator/Creating Biobrick
From 2011.igem.org
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<li class="current"><a href="/Team:UPO-Sevilla/Foundational_Advances/Biobrick_Creator/Creating_Biobrick" style="white-space: nowrap; float: left;">Creating a new Biobrick</a><ul></ul></li> | <li class="current"><a href="/Team:UPO-Sevilla/Foundational_Advances/Biobrick_Creator/Creating_Biobrick" style="white-space: nowrap; float: left;">Creating a new Biobrick</a><ul></ul></li> | ||
</ul> | </ul> | ||
| - | <a href="https://static.igem.org/mediawiki/2011/ | + | <a href="https://static.igem.org/mediawiki/2011/c/c2/UPO-Sevilla_BioBrickCreator1.0.5.zip" title="BioBrick Creator"><img style="margin: 0em 0em 2.5em;" class="imgright" src="https://static.igem.org/mediawiki/2011/9/97/UPOSevillaDownloadIcon.png" title="Download BioBrick Creator" width="200px"></a> |
<h1>Creating a new BioBrick</h1> | <h1>Creating a new BioBrick</h1> | ||
<h2>How does it work?</h2> | <h2>How does it work?</h2> | ||
| - | + | <p> | |
| - | <p> | + | |
| - | + | ||
There are three levels of BioBrick parts: parts, devices and systems. | There are three levels of BioBrick parts: parts, devices and systems. | ||
Parts are the building blocks and encode basic biological functions | Parts are the building blocks and encode basic biological functions | ||
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<ul> | <ul> | ||
<li>Protein Sequence Coding</li> | <li>Protein Sequence Coding</li> | ||
| - | <p>Protein coding sequences encode the amino acid sequence of a particular protein. Note that some protein coding sequences only encode a protein domain or half a protein. Others encode a full-length protein from start codon to stop codon.</p> | + | <p>Protein coding sequences encode the amino acid sequence of a particular protein. Note that some protein coding sequences only encode a protein domain or half a protein. Others encode a full-length protein from start codon to stop codon. Besides, you can choose a codon optimization depending on a specific organism.</p> |
<li>DNA</li> | <li>DNA</li> | ||
<p>DNA sequence with a known function which can't be automatically optimized due to possible functional losses.</p> | <p>DNA sequence with a known function which can't be automatically optimized due to possible functional losses.</p> | ||
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<p>DNA parts provide functionality to the DNA itself. DNA parts include cloning sites, scars, primer binding sites, spacers, recombination sites, conjugative tranfer elements, transposons, origami, and aptamers.</p> | <p>DNA parts provide functionality to the DNA itself. DNA parts include cloning sites, scars, primer binding sites, spacers, recombination sites, conjugative tranfer elements, transposons, origami, and aptamers.</p> | ||
</ul> | </ul> | ||
| - | <p>To use your own sequence, | + | <p>To use your own sequence, simply write or paste your raw DNA sequence in 5'-3' or upload your raw DNA sequence using TXT or FASTA format files. |
For FASTA format files, if your files have incorrect or missing characters, it has to be | For FASTA format files, if your files have incorrect or missing characters, it has to be | ||
fixed beforehand. For multiFASTA files, this aplication only reads the first sequence | fixed beforehand. For multiFASTA files, this aplication only reads the first sequence | ||
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erase the information not useful.</p> | erase the information not useful.</p> | ||
| - | </ | + | <h2>Interface</h2> |
| + | <div class="center"> | ||
| + | <img width="550" height="550" src="https://static.igem.org/mediawiki/2011/b/b0/UPO-Sevilla_BBC_module1screen.png"> | ||
| + | </div> | ||
| + | |||
<h2>UML Class Diagram</h2> | <h2>UML Class Diagram</h2> | ||
<div class="center"> | <div class="center"> | ||
<img src="https://static.igem.org/mediawiki/2011/d/d2/UPO-Sevilla_BBC_Module1.png"> | <img src="https://static.igem.org/mediawiki/2011/d/d2/UPO-Sevilla_BBC_Module1.png"> | ||
</div> | </div> | ||
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</div> | </div> | ||
Latest revision as of 13:35, 28 October 2011
Creating a new BioBrick
How does it work?
There are three levels of BioBrick parts: parts, devices and systems. Parts are the building blocks and encode basic biological functions (such as encoding a certain protein, or providing a promoter to let RNA polymerase bind and initiate transcription of downstream sequences.
You can choose what part type you want convert to BioBrick standard, you can tick between:
- Protein Sequence Coding
- DNA
- Characterized Sequence
Protein coding sequences encode the amino acid sequence of a particular protein. Note that some protein coding sequences only encode a protein domain or half a protein. Others encode a full-length protein from start codon to stop codon. Besides, you can choose a codon optimization depending on a specific organism.
DNA sequence with a known function which can't be automatically optimized due to possible functional losses.
DNA parts provide functionality to the DNA itself. DNA parts include cloning sites, scars, primer binding sites, spacers, recombination sites, conjugative tranfer elements, transposons, origami, and aptamers.
To use your own sequence, simply write or paste your raw DNA sequence in 5'-3' or upload your raw DNA sequence using TXT or FASTA format files. For FASTA format files, if your files have incorrect or missing characters, it has to be fixed beforehand. For multiFASTA files, this aplication only reads the first sequence For TXT files, aplication will read every character inside the file, so after it has to erase the information not useful.
Interface
UML Class Diagram
Sponsors
