Talk:Team:EPF-Lausanne/Protocols

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{{:Team:EPF-Lausanne/Templates/Header|title=Agarose gel electrophoresis}}
{{:Team:EPF-Lausanne/Templates/Header|title=Agarose gel electrophoresis}}
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made on 01.07.2011
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Written on 01.07.2011
== Purpose ==
== Purpose ==
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==Ingredients==
==Ingredients==
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for a 1% agarose gel
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For a 1% agarose gel
{| class="wikitable" style="text-align:center; width:80%;"
{| class="wikitable" style="text-align:center; width:80%;"
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|2.5µl
|2.5µl
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|-
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! scope=row | Gel Red
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! scope=row | GelRed
|2g
|2g
|200ml
|200ml
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|}
|}
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== Method ==
 
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<p>•      Weight agarose, place it into a bottle with a cap, add TBE 1x buffer</p>
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For sample loading:  RedSafe
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== Method ==
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* To prepare the gel:
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**    Weight agarose, place it into a bottle with a cap, add TBE 1x buffer
<p>•      Place the bottle into a microwave, <gras>loosen the cap</gras>, heat it untill it almost boils and the agar is dissolved</p>
<p>•      Place the bottle into a microwave, <gras>loosen the cap</gras>, heat it untill it almost boils and the agar is dissolved</p>
<p>• Cool it down until it can be hold (that can be done under water)</p>
<p>• Cool it down until it can be hold (that can be done under water)</p>
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<p>• Add 1.25µl of Gel Red</p>
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<p>• Add 1.25µl of GelRed</p>
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<p>• Pour into the rack, put the comb and wait while it polymerizes</p>
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<p>• Pour into the rack, put the comb and wait until it polymerizes</p>
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<p> '' Usually “B” valves are starting to load earlier (leave the button unpressurised prior to the pressure increase, it tends to stick to the slide.)</p>
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<p>  Increase the pressure in both “A” and “B” valves till ~13-15 psi and make sure that you can see that all chambers are separated. If no – increase the pressure''</p>
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{{:Team:EPF-Lausanne/Templates/Footer}}
{{:Team:EPF-Lausanne/Templates/Footer}}

Revision as of 20:39, 1 July 2011

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