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<h2 class="title">Operation ECHO </a></h2>
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<P ALIGN=CENTER><FONT FACE="Times New Roman, serif"><SPAN LANG="en-US"><A HREF="https://2011.igem.org/Team:Tsinghua-A/Project/echo"><FONT COLOR="#99284c"><FONT FACE="Arial, sans-serif"><FONT SIZE=4 STYLE="font-size: 15pt"><SPAN LANG="en-US">Operation
 +
ECHO</SPAN></FONT></FONT></FONT></A><FONT COLOR="#6b4794"><FONT FACE="Times New Roman, serif"><SPAN LANG="en-US">
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</SPAN></FONT></FONT><FONT COLOR="#6b4794"><FONT FACE="Arial, sans-serif"><FONT SIZE=4 STYLE="font-size: 15pt"><SPAN LANG="en-US">|
 +
</SPAN></FONT></FONT></FONT><A HREF="https://2011.igem.org/Team:Tsinghua-A/Project/col"><FONT COLOR="#99284c"><FONT FACE="Arial, sans-serif"><FONT SIZE=4 STYLE="font-size: 15pt"><SPAN LANG="en-US">Collaboration
 +
with other teams</SPAN></FONT></FONT></FONT></A></SPAN></FONT></P>
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<P ALIGN=CENTER><BR><BR>
 +
</P>
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<P ALIGN=CENTER><BR><BR>
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</P>
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<P ALIGN=LEFT STYLE="margin-left: 0.56cm"><FONT COLOR="#800000"><FONT FACE="Times New Roman, serif"><FONT SIZE=6 STYLE="font-size: 22pt"><SPAN LANG="en-US"><FONT FACE="Arial, sans-serif"><SPAN LANG="en-US">Operation
 +
ECHO, by Tsinghua-A Team</SPAN></FONT></SPAN></FONT></FONT></FONT></P>
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<P ALIGN=RIGHT STYLE="margin-left: 3.67cm"><FONT COLOR="#b84700"><FONT FACE="Times New Roman, serif"><FONT SIZE=6 STYLE="font-size: 22pt"><SPAN LANG="en-US"><A HREF="https://2011.igem.org/Team:Tsinghua-A/Project/echo"><FONT FACE="Arial, sans-serif"><FONT SIZE=5><SPAN LANG="en-US">More
 +
Details</SPAN></FONT></FONT></A></SPAN></FONT></FONT></FONT></P>
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<P ALIGN=LEFT STYLE="margin-left: 3.08cm"><FONT COLOR="#94476b"><FONT FACE="Times New Roman, serif"><SPAN LANG="en-US"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">During
 +
the season, we will dedicate in pursuing the goal of the construction
 +
of a biological oscillator, which we call &lsquo;ECHO&rsquo;, the
 +
abbreviation of &lsquo;the E.Coli Homochronous Oscillator&rsquo;.</SPAN></FONT></FONT></SPAN></FONT></FONT></P>
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<P ALIGN=LEFT STYLE="margin-left: 3.08cm"><FONT COLOR="#94476b"><FONT FACE="Times New Roman, serif"><SPAN LANG="en-US"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">With
 +
two Escherichia coli populations expressing gene one after another,
 +
they give red and green fluorescent light alternately. E.coli
 +
populations communicate bi-directionally by a class of signaling
 +
molecules involves in bacteria quorum sensing, that is, N-Acyl
 +
homoserine lactones (AHL), to regulate the gene expression of each
 +
other. By engineering their gene circuits, two groups (name as CELL-A
 +
and CELL-B) will form a network, with B inducing A and A restricting
 +
B, thus able to realize oscillation. A mechanism is set up to change
 +
the rate of an AHL expression, allowing us to control the period and
 +
the phase of the oscillatory cycle.</SPAN></FONT></FONT></SPAN></FONT></FONT></P>
 +
<P ALIGN=LEFT STYLE="margin-left: 3.08cm"><FONT COLOR="#94476b"><FONT FACE="Times New Roman, serif"><SPAN LANG="en-US"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">What&rsquo;s
 +
more, we establish a mathematical model to analyze the dynamics of
 +
the system, and computer simulation software is introduced into the
 +
process. The comparison between the wet lab experiment and the
 +
simulating outcome contributes to a contemplated and well-planned
 +
designation of our project.</SPAN></FONT></FONT></SPAN></FONT></FONT></P>
 +
<P ALIGN=LEFT STYLE="margin-left: 5.82cm"><BR><BR>
 +
</P >
 +
<P ALIGN=CENTER ><IMG SRC="https://static.igem.org/mediawiki/2011/thumb/0/01/Snap27.jpg/800px-Snap27.jpg" ></P >
 +
<P ALIGN=LEFT STYLE="margin-left: 5.82cm"><BR><BR>
 +
</P>
 +
<P ALIGN=LEFT STYLE="margin-left: 5.82cm"><BR><BR>
 +
</P>
 +
<P ALIGN=LEFT STYLE="margin-left: 0.6cm"><FONT COLOR="#6b2394"><FONT FACE="Times New Roman, serif"><FONT SIZE=6 STYLE="font-size: 22pt"><SPAN LANG="en-US"><FONT FACE="Arial, sans-serif"><SPAN LANG="en-US">Col</SPAN></FONT><FONT FACE="Arial, sans-serif"><SPAN LANG="en-US">laboration
 +
work with <A HREF="https://2011.igem.org/Team:Tsinghua">Team Tsinghua
 +
</A></SPAN></FONT></SPAN></FONT></FONT></FONT></P>
 +
<P ALIGN=LEFT STYLE="margin-left: 3.67cm; margin-bottom: 0cm"><BR>
 +
</P>
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<P ALIGN=RIGHT STYLE="margin-left: 3.67cm"><FONT COLOR="#b84700"><FONT FACE="Times New Roman, serif"><FONT SIZE=6 STYLE="font-size: 22pt"><SPAN LANG="en-US"><A HREF="https://2011.igem.org/Team:Tsinghua-A/Project/col"><FONT FACE="Arial, sans-serif"><FONT SIZE=5><SPAN LANG="en-US">More
 +
Details</SPAN></FONT></FONT></A></SPAN></FONT></FONT></FONT></P>
 +
<P ALIGN=LEFT STYLE="margin-left: 3.08cm"><FONT COLOR="#800000"><FONT FACE="Times New Roman, serif"><FONT SIZE=6 STYLE="font-size: 22pt"><SPAN LANG="en-US"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">In
 +
iGem 2011 tournament, we offered our help to another team from
 +
Tsinghua, which is comprised of underguaduates from the Dept of Life
 +
Science in Tsinghua University. </SPAN></FONT></FONT></SPAN></FONT></FONT></FONT>
 +
</P>
-
== Why we name after ‘ECHO’? ==
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<P ALIGN=LEFT STYLE="margin-left: 3.08cm"><FONT COLOR="#800000"><FONT FACE="Times New Roman, serif"><FONT SIZE=6 STYLE="font-size: 22pt"><SPAN LANG="en-US"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">We
-
</p><p>
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help them build the analysis Modeling of their bio-systems. </SPAN></FONT></FONT></SPAN></FONT></FONT></FONT>
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I guess that most of us have heard the Greek mythology of the love between nymph Echo and Narcissus. The voice of Echo remains hovering in the valley in the end of the story, from then on, ‘echo’ starts to have a meaning as reverberation or response. In the field of automation, we use ‘feedback’ to refer the process of response in a system, that is, the process in which part of the output is returned to its input in order to regulate its further output, just as an echo.
+
</P>
-
Analogously, ‘echo’ plays a significant role in our design. CELL-A generates AHLs, sending a message to CELL-B, and after B expresses the particular gene, B will ‘echo’ the message to respond. The ECHO part contributes to the communication and well-function of the system.</p>
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<p align="CENTER"><img src="https://static.igem.org/mediawiki/2011/1/11/Thu_col_main.jpg"></p>
-
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<p>What does ‘homochronous’ refer?</p>
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<P ALIGN=LEFT STYLE="margin-left: 5.82cm"><BR><BR>
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<p>The prefix ‘homo’ represents ‘same’, while ‘chrono’ means ‘time-related’. As a jargon of Information, the word ‘homochronous’ describes the relationship between two signals that their corresponding significant instants are displaced by a constant interval of time. Here we use this term to depict the relationship between CELL-A & B. That is to say, in the process of oscillating, the AHL expression rate of CELL-A and that of B (also RED and GREEN fluorescent signals) share the same period, or fluctuate in a same pace. In addition, the interval of two signals remains constant, so the RED signal comes out while GREEN fades away at the same time and vice versa. Consequently, time goes, RED and GREEN flare alternately.</p>
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</P>
-
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<p>&nbsp;</p><p>&nbsp;</p><p>&nbsp;</p>
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<p>How does the system work?</p>
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<br><br><br><br>
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<p>The Ring Oscillator is a device in digital electronic circuits, and it’s composed of an odd number of NOT gates or inverters attaching in a chain, with the last inverter is fed back into the first. Because of the odd number, the final output is the logical NOT of the first input, and the delay as well as the feedback cause the oscillation.
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<p align="CENTER"><a href="https://2011.igem.org/Team:Tsinghua-A"><img src="https://static.igem.org/mediawiki/2011/9/92/Killbanner_header.jpg" alt="" width="960"/><a href="https://2011.igem.org"><img src="https://static.igem.org/mediawiki/igem.org/2/29/Killbanner_header2.jpg" alt="" width="960"/></p>
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When the idea is applied in the synthetic biology, we use two populations of E.coli to construct a system, in which CELL-B induces A and A restricts B. To explain it an electronic way, CELL- A acts as a NOT gate while B as a transfer gate, forming a feedback network with one (of course is odd) converter, with a delay time of biological reaction. Then, theoretically, the system will oscillate.</p>
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Latest revision as of 04:16, 28 October 2011

JavaScript Slideshow - TinySlideshow

Operation ECHO | Collaboration with other teams





Operation ECHO, by Tsinghua-A Team

More Details

During the season, we will dedicate in pursuing the goal of the construction of a biological oscillator, which we call ‘ECHO’, the abbreviation of ‘the E.Coli Homochronous Oscillator’.

With two Escherichia coli populations expressing gene one after another, they give red and green fluorescent light alternately. E.coli populations communicate bi-directionally by a class of signaling molecules involves in bacteria quorum sensing, that is, N-Acyl homoserine lactones (AHL), to regulate the gene expression of each other. By engineering their gene circuits, two groups (name as CELL-A and CELL-B) will form a network, with B inducing A and A restricting B, thus able to realize oscillation. A mechanism is set up to change the rate of an AHL expression, allowing us to control the period and the phase of the oscillatory cycle.

What’s more, we establish a mathematical model to analyze the dynamics of the system, and computer simulation software is introduced into the process. The comparison between the wet lab experiment and the simulating outcome contributes to a contemplated and well-planned designation of our project.







Collaboration work with Team Tsinghua


More Details

In iGem 2011 tournament, we offered our help to another team from Tsinghua, which is comprised of underguaduates from the Dept of Life Science in Tsinghua University.

We help them build the analysis Modeling of their bio-systems.



 

 

 





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