Team:Grenoble/Projet/Biobricks
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- | <center><h5>MerR-del : K545103</h5></center><br/> | + | <center><h5>MerR-del : (K545103)</h5></center><br/><br/> |
+ | |||
MerR (BBa_K346001) without RBS<br/> | MerR (BBa_K346001) without RBS<br/> | ||
- | PCR gel<br/> | + | PCR gel<br/><br/> |
+ | |||
Well 1: Positive control (pSB4K5)<br/> | Well 1: Positive control (pSB4K5)<br/> | ||
Well 2: Negative control (Without DNA)<br/> | Well 2: Negative control (Without DNA)<br/> | ||
- | + | Wells 3, 4, 5, 6, 7: MerR-del (expected at 740pb, obtain around 700-800pb)<br/><br/> | |
- | Confirmed by sequencing | + | |
+ | Confirmed by sequencing<br/> | ||
</p> | </p> | ||
</td> | </td> | ||
+ | |||
<td> | <td> | ||
<center><img src="https://static.igem.org/mediawiki/2011/f/fe/01.png"/></center> | <center><img src="https://static.igem.org/mediawiki/2011/f/fe/01.png"/></center> | ||
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<p align="left"> | <p align="left"> | ||
- | <center><h5>pMerT-GFP: K545002</h5></center><br/><br/> | + | <center><h5>pMerT-GFP: (K545002)</h5></center><br/><br/> |
+ | |||
Original parts: K346002, E0240<br/> | Original parts: K346002, E0240<br/> | ||
PCR gel<br/><br/> | PCR gel<br/><br/> | ||
- | Well 1 | + | |
- | Well 3: pMerT-GFP (expected | + | Well 1: MW<br/> |
- | Confirmed by sequencing | + | Well 2: empty well<br/> |
+ | Well 3 and 4: failed clonning<br/> | ||
+ | Well 5: pMerT-GFP (expected at 1200pb obtain just over 1000pb)<br/><br/> | ||
+ | |||
+ | Confirmed by sequencing<br/> | ||
</p> | </p> | ||
</td> | </td> | ||
+ | |||
<td> | <td> | ||
<center><img src="https://static.igem.org/mediawiki/2011/d/d9/02.png"/></center> | <center><img src="https://static.igem.org/mediawiki/2011/d/d9/02.png"/></center> | ||
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<p align="left"> | <p align="left"> | ||
- | <center><h5>pCin-CrtEBI (K545000)</h5></center><br/><br/> | + | <center><h5>pCin-CrtEBI (K545000)</h5></center><br/><br/> |
+ | |||
Originally: R0077, K274100<br/> | Originally: R0077, K274100<br/> | ||
- | PCR gel<br/> | + | PCR gel<br/><br/> |
- | Expected at 3500pb, obtain between 3000 and 4000pb<br/> | + | |
- | Confirmed by sequencing | + | Wells 1, 2 and 3: pCin-CrtEBI (Expected at 3500pb, obtain between 3000 and 4000pb)<br/> |
+ | Well 4: MW<br/><br/> | ||
+ | |||
+ | Confirmed by sequencing<br/> | ||
</td> | </td> | ||
+ | |||
<td> | <td> | ||
<center><img src="https://static.igem.org/mediawiki/2011/9/9a/03.png"/></center> | <center><img src="https://static.igem.org/mediawiki/2011/9/9a/03.png"/></center> | ||
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<p align="left"> | <p align="left"> | ||
<center><h5>pConst-RBS-CinR (K545004)</h5></center><br/><br/> | <center><h5>pConst-RBS-CinR (K545004)</h5></center><br/><br/> | ||
+ | |||
Originally J23119, B0034 C0077<br/> | Originally J23119, B0034 C0077<br/> | ||
- | Restriction gel<br/> | + | Restriction gel<br/><br/> |
- | + | ||
- | + | Wells 1 and 2: pConst-CinR(expected at 950pb)<br/> | |
+ | Wells 4 and 5: failed cloning<br/> | ||
+ | Wells 3 and 6: empty wells<br/> | ||
+ | plasmids expected about 2 000pb, and are obtain for each well.<br/><br/> | ||
+ | |||
Confirmed by sequencing<br/> | Confirmed by sequencing<br/> | ||
</td> | </td> | ||
+ | |||
<td> | <td> | ||
<center><img src="https://static.igem.org/mediawiki/2011/9/96/04.png"/></center> | <center><img src="https://static.igem.org/mediawiki/2011/9/96/04.png"/></center> | ||
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<p align="left"> | <p align="left"> | ||
<center><h5>pLac-CinR (S04607)</h5></center><br/><br/> | <center><h5>pLac-CinR (S04607)</h5></center><br/><br/> | ||
+ | |||
Originally R0011, E0240<br/> | Originally R0011, E0240<br/> | ||
- | pConst-GFP (K545001)<br/> | + | Restriction gel<br/><br/> |
+ | |||
+ | <center><h5>pConst-GFP (K545001)</h5></center><br/><br/> | ||
+ | |||
Originally J23119, E0240<br/> | Originally J23119, E0240<br/> | ||
- | Restriction gel<br/> | + | Restriction gel<br/><br/> |
+ | |||
Well 1: pLac-CinR (expected at 950pb)<br/> | Well 1: pLac-CinR (expected at 950pb)<br/> | ||
Well 2: pConst-CinR (expected at 900pb)<br/> | Well 2: pConst-CinR (expected at 900pb)<br/> | ||
- | Confirmed by sequencing | + | plasmids expected about 2 000pb, and are obtain for each well.<br/><br/> |
+ | |||
+ | Confirmed by sequencing<br/> | ||
</td> | </td> | ||
+ | |||
<td> | <td> | ||
<center><img src="https://static.igem.org/mediawiki/2011/7/79/05.png"/></center> | <center><img src="https://static.igem.org/mediawiki/2011/7/79/05.png"/></center> | ||
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<p align="left"> | <p align="left"> | ||
<center><h5>RBS-CinR (S04602)</h5></center><br/><br/> | <center><h5>RBS-CinR (S04602)</h5></center><br/><br/> | ||
+ | |||
Originally B0034, C0077<br/> | Originally B0034, C0077<br/> | ||
- | PCR gel<br/> | + | PCR gel<br/><br/> |
+ | |||
Well 1: RBS-CinR (expected at 1000)<br/> | Well 1: RBS-CinR (expected at 1000)<br/> | ||
- | Confirmed by sequencing | + | Wells 2, 3 and 4: failed clonning<br/><br/> |
+ | |||
+ | Confirmed by sequencing<br/> | ||
</td> | </td> | ||
+ | |||
<td> | <td> | ||
<center><img src="https://static.igem.org/mediawiki/2011/5/5f/06.png"/></center> | <center><img src="https://static.igem.org/mediawiki/2011/5/5f/06.png"/></center> | ||
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<p align="left"> | <p align="left"> | ||
<center><h5>RBS-TetR (S04603)</h5></center><br/><br/> | <center><h5>RBS-TetR (S04603)</h5></center><br/><br/> | ||
+ | |||
Originally B0034, C0040<br/> | Originally B0034, C0040<br/> | ||
- | PCR gel<br/> | + | PCR gel<br/><br/> |
- | RBS-TetR (expected at 950pb)<br/> | + | |
+ | well 1: RBS-TetR (expected at 950pb)<br/><br/> | ||
+ | |||
Confirmed by sequencing<br/> | Confirmed by sequencing<br/> | ||
</td> | </td> | ||
+ | |||
<td> | <td> | ||
<center><img src="https://static.igem.org/mediawiki/2011/a/af/07.png"/></center> | <center><img src="https://static.igem.org/mediawiki/2011/a/af/07.png"/></center> |
Revision as of 15:04, 27 October 2011
Our Biobricks:
- Here you can find all our news and composites parts we send to the registry.
<groupparts>iGEM011 Grenoble</groupparts>
- You can also find in the registry some of our constructions intermediates:
BBa_S04602 RBS-CinR, BBa_B0034+BBa_C0077
BBa_S04603 RBS-TetR, BBa_B0034+BBa_C0040
BBa_S04606 RBS-TetR-RBS-CinR, BBa_B0034+BBa_C0040+BBa_B0034+BBa_C0077
BBa_S04605 RBS-TetR-RBS-GFP, BBa_B0034+BBa_C0077+BBa_E0240
BBa_S04607 pLac-CinR, BBa_R0011+BBa_C0077
BBa_S04618 pLac-CinR, BBa_K545006+BBa_I13401
MerR-del : (K545103)MerR (BBa_K346001) without RBS PCR gel Well 1: Positive control (pSB4K5) Well 2: Negative control (Without DNA) Wells 3, 4, 5, 6, 7: MerR-del (expected at 740pb, obtain around 700-800pb) Confirmed by sequencing |
|
pMerT-GFP: (K545002)Original parts: K346002, E0240 PCR gel Well 1: MW Well 2: empty well Well 3 and 4: failed clonning Well 5: pMerT-GFP (expected at 1200pb obtain just over 1000pb) Confirmed by sequencing |
|
pCin-CrtEBI (K545000)Originally: R0077, K274100 PCR gel Wells 1, 2 and 3: pCin-CrtEBI (Expected at 3500pb, obtain between 3000 and 4000pb) Well 4: MW Confirmed by sequencing |
|
pConst-RBS-CinR (K545004)Originally J23119, B0034 C0077 Restriction gel Wells 1 and 2: pConst-CinR(expected at 950pb) Wells 4 and 5: failed cloning Wells 3 and 6: empty wells plasmids expected about 2 000pb, and are obtain for each well. Confirmed by sequencing |
|
pLac-CinR (S04607)Originally R0011, E0240 Restriction gel pConst-GFP (K545001)Originally J23119, E0240 Restriction gel Well 1: pLac-CinR (expected at 950pb) Well 2: pConst-CinR (expected at 900pb) plasmids expected about 2 000pb, and are obtain for each well. Confirmed by sequencing |
|
RBS-CinR (S04602)Originally B0034, C0077 PCR gel Well 1: RBS-CinR (expected at 1000) Wells 2, 3 and 4: failed clonning Confirmed by sequencing |
|
RBS-TetR (S04603)Originally B0034, C0040 PCR gel well 1: RBS-TetR (expected at 950pb) Confirmed by sequencing |
|
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