Team:Grenoble/Projet/Biobricks

From 2011.igem.org

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      <p align="left">
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      MerR-del : K545103.
 +
      MerR (BBa_K346001) without RBS
 +
      PCR gel
 +
      Well 1: Positive control (pSB4K5)
 +
      Well 2: Negative control (Without DNA)
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      Well 3, 4, 5, 6, 7: MerR-del (expected at 740pb, obtain around 700-800pb)
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      Confirmed by sequencing
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      </p>
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    image
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  </td>
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    pMerT-GFP: K545002
 +
    Original parts: K346002, E0240
 +
    PCR gel
 +
    Well 1 and 2: Failed cloning
 +
    Well 3: pMerT-GFP (expected at1200pb obtain just over 1000pb)
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    Confirmed by sequencing
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    </p>
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  </td>
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  <td>
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image
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  </td>
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    pCin-CrtEBI (K545000) 
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    Originally: R0077, K274100
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    PCR gel
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    Expected at 3500pb, obtain between 3000 and 4000pb
 +
    Confirmed by sequencing
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  </td>
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  <td>
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image
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  </td>
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</tr>
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<tr>
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  <td>
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    <p align="left">
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    pConst-RBS-CinR (K545004)
 +
    Originally J23119, B0034 C0077
 +
    Restriction gel
 +
    Well 1, 2: failed cloning
 +
    Well 3, 4: pConst-CinR(expected at 950pb)
 +
    Confirmed by sequencing
 +
  </td>
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  <td>
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image
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  </td>
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</tr>
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<tr>
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  <td>
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    <p align="left">
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    pLac-CinR (S04607)
 +
    Originally R0011, E0240
 +
    pConst-GFP (K545001)
 +
    Originally J23119, E0240
 +
    Restriction gel
 +
    Well 1: pLac-CinR (expected at 950pb)
 +
    Well 2: pConst-CinR (expected at 900pb)
 +
    Confirmed by sequencing
 +
  </td>
 +
  <td>
 +
image
 +
  </td>
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</tr>
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<tr>
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  <td>
 +
    <p align="left">
 +
    RBS-CinR (S04602)
 +
    Originally B0034, C0077
 +
    PCR gel
 +
    Well 1: RBS-CinR (expected at 1000)
 +
    Confirmed by sequencing
 +
  </td>
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  <td>
 +
image
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  </td>
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</tr>
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<tr>
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  <td>
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    <p align="left">
 +
    RBS-TetR (S04603)
 +
    Originally B0034, C0040
 +
    PCR gel
 +
    RBS-TetR (expected at 950pb)
 +
    Confirmed by sequencing
 +
  </td>
 +
  <td>
 +
image
 +
  </td>
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</tr>
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</table>
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              </div>
</div>
</div>
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Revision as of 17:15, 24 October 2011

Grenoble 2011, Mercuro-Coli iGEM


Our Biobricks:

  • Here you can find all our news and composites parts we send to the registry.

<groupparts>iGEM011 Grenoble</groupparts> 

              		
			

				
    
				
  • You can also find in the registry some of our constructions intermediates:
    • 
				

				
				

				

	

		BBa_S04602
		
RBS-CinR, BBa_B0034+BBa_C0077 

	

	

		BBa_S04603
		
RBS-TetR, BBa_B0034+BBa_C0040 

	
  
	

		BBa_S04606
		
RBS-TetR-RBS-CinR, BBa_B0034+BBa_C0040+BBa_B0034+BBa_C0077

	

	

		BBa_S04605
		
RBS-TetR-RBS-GFP, BBa_B0034+BBa_C0077+BBa_E0240

	

	

		BBa_S04607
		
pLac-CinR, BBa_R0011+BBa_C0077

	
 
	

		BBa_S04618
		
pLac-CinR, BBa_K545006+BBa_I13401

	
 


		

			

                


                   



   
      

      MerR-del : K545103.
      MerR (BBa_K346001) without RBS
      PCR gel
      Well 1: Positive control (pSB4K5)
      Well 2: Negative control (Without DNA)
      Well 3, 4, 5, 6, 7: MerR-del (expected at 740pb, obtain around 700-800pb)
      Confirmed by sequencing
      

   		
   
     image
   





   
     

     pMerT-GFP: K545002
     Original parts: K346002, E0240
     PCR gel
     Well 1 and 2: Failed cloning
     Well 3: pMerT-GFP (expected at1200pb obtain just over 1000pb)
     Confirmed by sequencing
     

   		
   
image
   





   
     

     pCin-CrtEBI (K545000)  
     Originally: R0077, K274100
     PCR gel
     Expected at 3500pb, obtain between 3000 and 4000pb
     Confirmed by sequencing
   

   
image
   





   
     

     pConst-RBS-CinR (K545004)
     Originally J23119, B0034 C0077
     Restriction gel
     Well 1, 2: failed cloning
     Well 3, 4: pConst-CinR(expected at 950pb)
     Confirmed by sequencing
   

   
image
   





   
     

     pLac-CinR (S04607)
     Originally R0011, E0240
     pConst-GFP (K545001)
     Originally J23119, E0240
     Restriction gel
     Well 1: pLac-CinR (expected at 950pb)
     Well 2: pConst-CinR (expected at 900pb)
     Confirmed by sequencing
   

   
image
   





   
     

     RBS-CinR (S04602)
     Originally B0034, C0077
     PCR gel
     Well 1: RBS-CinR (expected at 1000)
     Confirmed by sequencing
   

   
image
   





   
     

     RBS-TetR (S04603)
     Originally B0034, C0040
     PCR gel
     RBS-TetR (expected at 950pb)
     Confirmed by sequencing
   

   
image
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