Team:WITS-CSIR SA/Collaboration/Index

From 2011.igem.org

(Difference between revisions)
 
(17 intermediate revisions not shown)
Line 1: Line 1:
-
<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" "http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd">
+
[[Media:Example.ogg]]<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" "http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd">
<html xmlns="http://www.w3.org/1999/xhtml">
<html xmlns="http://www.w3.org/1999/xhtml">
<head>
<head>
Line 15: Line 15:
         div.top
         div.top
         {
         {
-
             height: 120px;
+
             height: 95px;
             background-color: #2d4782;
             background-color: #2d4782;
             filter: progid:DXImageTransform.Microsoft.gradient(startColorstr='#6279ad', endColorstr='#2d4782'); /* for IE */
             filter: progid:DXImageTransform.Microsoft.gradient(startColorstr='#6279ad', endColorstr='#2d4782'); /* for IE */
Line 50: Line 50:
     <link rel="stylesheet" type="text/css" href="https://2011.igem.org/Team:WITS-CSIR_SA/css/theme/jquery-ui-1.8.14.custom.css?action=raw&amp;ctype=text/css">
     <link rel="stylesheet" type="text/css" href="https://2011.igem.org/Team:WITS-CSIR_SA/css/theme/jquery-ui-1.8.14.custom.css?action=raw&amp;ctype=text/css">
     <link rel="stylesheet" type="text/css" href="https://2011.igem.org/Team:WITS-CSIR_SA/css/liteaccordion.css?action=raw&amp;ctype=text/css">
     <link rel="stylesheet" type="text/css" href="https://2011.igem.org/Team:WITS-CSIR_SA/css/liteaccordion.css?action=raw&amp;ctype=text/css">
 +
<link href="http://ajax.googleapis.com/ajax/libs/dojo/1.6/dojox/image/resources/Lightbox.css" rel="stylesheet" type="text/css" />
 +
 +
<link href="https://2011.igem.org/Team:WITS-CSIR_SA/Styles/Dropdown.css?action=raw&amp;ctype=text/css" media="screen" rel="stylesheet" type="text/css" />
 +
<link rel="stylesheet" type="text/css" href="https://2011.igem.org/Team:WITS-CSIR_SA/Styles/default.css?action=raw&amp;ctype=text/css" />
 +
<link href="https://2011.igem.org/Team:WITS-CSIR_SA/Styles/default.advanced.css?action=raw&amp;ctype=text/css" media="screen" rel="stylesheet" type="text/css" />
 +
 +
<!--[if lt IE 7]>
 +
<script type="text/javascript" src="https://2011.igem.org/Team:WITS-CSIR_SA/Styles/jquery.dropdown.js?action=raw&amp;ctype=text/js"></script>
 +
<![endif]-->
 +
     <!-- Begin: Core -->
     <!-- Begin: Core -->
     <script type="text/javascript" src="https://2011.igem.org/Team:WITS-CSIR_SA/js/lib/jquery-1.5.1.min.js?action=raw&amp;ctype=text/js"></script>
     <script type="text/javascript" src="https://2011.igem.org/Team:WITS-CSIR_SA/js/lib/jquery-1.5.1.min.js?action=raw&amp;ctype=text/js"></script>
Line 84: Line 94:
         dojo.require("dijit.form.TextBox");
         dojo.require("dijit.form.TextBox");
         dojo.require("dojo.parser");
         dojo.require("dojo.parser");
 +
        dojo.require("dojox.image.Lightbox");
     </script>
     </script>
</head>
</head>
<body class="claro">
<body class="claro">
-
    <script type="text/javascript" src="https://2011.igem.org/Team:WITS-CSIR_SA/client/MenuModel.js?action=raw&amp;ctype=text/js"></script>
 
-
    <script type="text/javascript" src="https://2011.igem.org/Team:WITS-CSIR_SA/client/SideMenu.js?action=raw&amp;ctype=text/js"></script>
 
     <div data-dojo-type="dijit.layout.BorderContainer" data-dojo-props="design: 'headline', gutters: false"
     <div data-dojo-type="dijit.layout.BorderContainer" data-dojo-props="design: 'headline', gutters: false"
         class="container">
         class="container">
 +
<div style="position: absolute; width: 100%; top: 80px;">
 +
<ul class="dropdown dropdown-horizontal">
 +
<li><a href="https://2011.igem.org/Team:WITS-CSIR_SA">Home</a></li>
 +
<li><a href="#" class="dir">Project</a>
 +
<ul>
 +
<li><a href="https://2011.igem.org/Team:WITS-CSIR_SA/Project/Concept">Overview</a></li>
 +
<li><a href="https://2011.igem.org/Team:WITS-CSIR_SA/Characterization">Characterization</a></li>
 +
<li><a href="https://2011.igem.org/Team:WITS-CSIR_SA/Project/Modelling">Modelling</a></li>
 +
<li><a href="https://2011.igem.org/Team:WITS-CSIR_SA/Collaboration/Index">Collaboration</a></li>
 +
<li><a href="https://2011.igem.org/Team:WITS-CSIR_SA/Project/Notebook">Lab notebook</a></li>
 +
<li><a href="https://2011.igem.org/Team:WITS-CSIR_SA/Project/Applications">Potential applications</a></li>
 +
<li><a href="https://2011.igem.org/Team:WITS-CSIR_SA/Project/Achievements">Achievements</a></li>
 +
<li><a href="https://2011.igem.org/Team:WITS-CSIR_SA/Project/Protocols">Protocols</a></li>
 +
</ul>
 +
</li>
 +
<li><a href="https://2011.igem.org/Team:WITS-CSIR_SA/Parts">Parts submitted</a></li>
 +
<li><a href="https://2011.igem.org/Team:WITS-CSIR_SA/Characterization">Data</a></li>
 +
<li><a href="./" class="dir">Outreach</a>
 +
<ul>
 +
<li><a href="https://2011.igem.org/Team:WITS-CSIR_SA/Outreach/SciBono">Scibono experience</a></li>
 +
<li><a href="https://2011.igem.org/Team:WITS-CSIR_SA/Outreach/Forum">Synthetic biology forum</a></li>
 +
<li><a href="https://2011.igem.org/Team:WITS-CSIR_SA/Outreach/Survey">Survey</a></li>
 +
</ul>
 +
</li>
 +
<li><a href="https://2011.igem.org/Team:WITS-CSIR_SA/Project/Safety">Safety</a></li>
 +
 +
<li><a href="https://2011.igem.org/Team:WITS-CSIR_SA/Diary/Gallery">Gallery</a></li>
 +
<li><a href="./" class="dir">About us</a>
 +
<ul>
 +
<li><a href="https://2011.igem.org/Team:WITS-CSIR_SA/AboutUs/MeetTheTeam">Meet the team</a></li>
 +
<li><a href="https://2011.igem.org/Team:WITS-CSIR_SA/Outreach/PublicRelations">Media attention</a></li>
 +
<li><a href="https://2011.igem.org/Team:WITS-CSIR_SA/AboutUs/Attributions">Attributions</a></li>
 +
<li><a href="https://2011.igem.org/Team:WITS-CSIR_SA/AboutUs/Acknowledgements">Acknowledgements</a></li>
 +
<li><a href="https://2011.igem.org/Team:WITS-CSIR_SA/AboutUs/MeetOurBugs">Meet our bugs!</a></li>
 +
<li><a href="https://2011.igem.org/Team:WITS-CSIR_SA/AboutUs/ContactUs">Contact us</a></li>
 +
</ul>
 +
</li>
 +
</ul>
 +
</div>
         <div data-dojo-type="dijit.layout.ContentPane" data-dojo-props="region: 'top'" class="top">
         <div data-dojo-type="dijit.layout.ContentPane" data-dojo-props="region: 'top'" class="top">
             <a href="https://2011.igem.org/Team:WITS-CSIR_SA">
             <a href="https://2011.igem.org/Team:WITS-CSIR_SA">
-
                 <img src="https://static.igem.org/mediawiki/2011/c/c9/Logo.png" alt="Biotweet" height="80px"
+
                 <img src="https://static.igem.org/mediawiki/2011/6/63/Wits_Logo_Biotweet_Rearrange.gif" alt="Biotweet" height="60px"
-
                     style="float: left; margin-bottom: 10px; border: none;" />
+
                     style="float: left; margin-bottom: 5px; border: none;" />
-
             </a><a href="https://igem.org/Main_Page" target="_blank">
+
             </a><a href="https://2011.igem.org/Main_Page" target="_blank">
-
                 <img src="https://static.igem.org/mediawiki/2011/b/be/ICL_iGEM_Logo.png" alt="iGem" height="80px"
+
                 <img src="https://static.igem.org/mediawiki/2011/b/be/ICL_iGEM_Logo.png" alt="iGem" height="60px"
-
                     style="float: right; margin-bottom: 10px; border: none;" />
+
                     style="float: right; margin-bottom: 5px; border: none;" />
             </a>
             </a>
-
             <div class="menu padding ui-helper-clearfix">
+
             <div class="ui-helper-clearfix">
             </div>
             </div>
         </div>
         </div>
         <div data-dojo-type="dijit.layout.ContentPane" data-dojo-props="region: 'center'"
         <div data-dojo-type="dijit.layout.ContentPane" data-dojo-props="region: 'center'"
             class="center">
             class="center">
 +
             <div style="width: 903px; margin: auto; height: 100%;">
             <div style="width: 903px; margin: auto; height: 100%;">
                 <div data-dojo-type="dijit.layout.BorderContainer" data-dojo-props="design: 'headline'"
                 <div data-dojo-type="dijit.layout.BorderContainer" data-dojo-props="design: 'headline'"
                     style="height: 100%;">
                     style="height: 100%;">
-
                 
 
                     <div data-dojo-type="dijit.layout.ContentPane" data-dojo-props="region: 'center'">
                     <div data-dojo-type="dijit.layout.ContentPane" data-dojo-props="region: 'center'">
                         <div style="height: 100%; overflow-y: auto;">
                         <div style="height: 100%; overflow-y: auto;">
-
<h1>
+
 
 +
<h1>
                   Collaboration</h1>
                   Collaboration</h1>
<div data-dojo-type="dijit.TitlePane" data-dojo-props="title:'Introduction'">
<div data-dojo-type="dijit.TitlePane" data-dojo-props="title:'Introduction'">
 +
<center><img src="https://static.igem.org/mediawiki/2011/4/4f/Collaboration_Imp_Plus_Wits.png"></center>
<p>The Wits CSIR iGEM team collaborated with Imperial College London, both for the modelling and the potential suitability of motility experiments in the testing of our machines. The biologists in our team and those at Imperial College London had a number of skype conferences to discuss the progress of our lab work as well as to share insight into both qualitative and quantitative assay for motility. </p>
<p>The Wits CSIR iGEM team collaborated with Imperial College London, both for the modelling and the potential suitability of motility experiments in the testing of our machines. The biologists in our team and those at Imperial College London had a number of skype conferences to discuss the progress of our lab work as well as to share insight into both qualitative and quantitative assay for motility. </p>
</div>
</div>
Line 121: Line 171:
<br>
<br>
<div data-dojo-type="dijit.TitlePane" data-dojo-props="title:'Modelling'">
<div data-dojo-type="dijit.TitlePane" data-dojo-props="title:'Modelling'">
-
<p>Collaboration was also performed between the engineers to improve the modeling for both the teams. Several Skype confefrences were held between the team from Imperial College London and the Wits CSIR team to discuss the design and theory behind our own chemotaxis models. The collaborative effort resulted in the Imperial College London kindly providing us with a model for our theophylline riboswitches. Their modelling results are as follows:</p>
+
<p>Collaboration was also performed between the engineers to improve the modeling for both the teams. Several Skype conferences were held between the team from Imperial College London and the Wits CSIR team to discuss the design and theory behind our own chemotaxis models. The collaborative effort resulted in the Imperial College London kindly providing us with a model for our theophylline riboswitches. Their modelling results can be found on our href="https://2011.igem.org/Team:WITS-CSIR_SA/Project/Modelling">modelling</a> page.
-
<p>The Wits CSIR iGEM team intend to use a riboswitch to reprogram the chemotactic behavior of <i>E.coli</i>. The project includes engineering the attraction of the bacteria to theophylline[1]. CheZ is an important protein controlling the chemotaxis of bacteria. They used a theophylline riboswitch to control the expression of CheZ in CheZ deletion mutants in order to engineer the bacteria's movement towards theophylline[1]. We're using a riboswitch sensitive to theophylline to control the expression of CheZ. In the absence of theophylline, the start codon is covered so the translation of the strand cannot occur. In the presence of theophylline, the conformation of the riboswitch changes and the ribosome binding site is exposed[1]. Thus, the higher the concentration of the theophylline, the more will enter the cell resulting in the up regulation of CheZ expression. This will increase the frequency of directed movement[1].</p>
+
-
<p>The theophylline riboswitch can be modeled in three differential equations [2].</p>
+
-
<p><center>M ̇= αϕ(T)-γM</center></p>
+
-
<p><center>(CheZ) ̇= βMΘ(P)-ξCheZ</center></p>
+
-
<p><center>T ̇= ς(P_ext )CheZ-δT</center></p>
+
 +
                           
                             </div>
                             </div>
Line 134: Line 180:
                 </div>
                 </div>
             </div>
             </div>
-
        </div>
+
</div>
         <div data-dojo-type="dijit.layout.ContentPane" data-dojo-props="region: 'bottom'"
         <div data-dojo-type="dijit.layout.ContentPane" data-dojo-props="region: 'bottom'"
             class="footerBox" style="background-color: #ffffff;">
             class="footerBox" style="background-color: #ffffff;">

Latest revision as of 20:24, 22 October 2011

Media:Example.ogg<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" "http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd"> Biotweet - Collaboration

Biotweet iGem

Collaboration

The Wits CSIR iGEM team collaborated with Imperial College London, both for the modelling and the potential suitability of motility experiments in the testing of our machines. The biologists in our team and those at Imperial College London had a number of skype conferences to discuss the progress of our lab work as well as to share insight into both qualitative and quantitative assay for motility.


We provided Imperial College London with some ideas regarding motility assays using TTC in stab agar. Their team gave us some tips about the preparation of cells for motility experiments and recommended spinning down the bacteria and resuspending them in a low volume of broth to increase the density of cells. They also recommended the use of capillary motility assays. There was an exchange of protocols both ways to aid in the set-up of our experiments. A week later we discussed the progress we had made in the lab. We gave each other tips and suggested more assays to indicate the ability of our bacteria to move towards a stimulus. Imperial College London shared with us a type of capillary assay they were performing at the time and we, too, shared a protocol with them which is detailed in Gullivan and Topps (2006) paper, Guiding bacteria with small molecules and RNA.


Collaboration was also performed between the engineers to improve the modeling for both the teams. Several Skype conferences were held between the team from Imperial College London and the Wits CSIR team to discuss the design and theory behind our own chemotaxis models. The collaborative effort resulted in the Imperial College London kindly providing us with a model for our theophylline riboswitches. Their modelling results can be found on our href="https://2011.igem.org/Team:WITS-CSIR_SA/Project/Modelling">modelling page.

Retrieved from "http://2011.igem.org/Team:WITS-CSIR_SA/Collaboration/Index"