Team:UQ-Australia/Parts

From 2011.igem.org

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{{:Team:UQ-Australia/Template:Header}}
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|rowspan="2"|The human circadian rhythm drives many important processes in the body in accordance with the sleep/wake cycle. A characteristic of this biological clock is the periodic oscillation of gene expression. Current parts in the Registry designed to regulate periodic oscillations of gene expression have shown limited success.
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Here we demonstrate a biological clock being standardised as a set of BioBrick parts.
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__NOTOC__
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Our network is controlled by an engineered promoter, Plac/ara, which features both an activator and a repressor domain. This controls the production of downstream genes to activate other inducible promoters, pBAD and GlnAp2, eventually leading to the production of a repressor protein, lacI, which inhibits Plac/ara, resulting in oscillatory expression. This project shows the feasibility of standardising the biological clock in E. coli and grounds further development for applications in regulated drug/hormone delivery and ion channel control.
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We were not completely successful in our experimental endeavours towards making the BioBrick parts to be submitted to the registry, however we were able to add BioBrick sites to each part listed below. Therefore, except for Plac/ara we had all the parts ready to be cloned into the iGEM submission vector pSB1C3. For more information on the laboratory work please see the [https://2011.igem.org/Team:UQ-Australia/Project '''Project page'''].
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! [[File:UQ-Australia_logo_2011.png|125x125px|link=https://2011.igem.org/Team:UQ-Australia]]
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===<span style="color:#558822"> Parts </span>===
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a:link { color: #603371; text-decoration: none}
 
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|width="100"|[[File:MultiplePromoter image.png|100x100px|link=https://2011.igem.org/File:Promoter_image.png#filelinks]]
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|width="250"|'''<span style="color:#D4A017">Plac/ara</span>'''
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A multi-domain promoter that is self-inducible by arabinose. Gene expression regulated by LacI binding to special operator sites in the promoter
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/*-----------------------------------------------------------------------------------------------*/
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|width="100"|[[File:Gene image.png|100x100px]]
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div.MenuBar ul li ul.DropDownMenu {
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|width="250"|'''<span style="color:#D4A017">glnG</span>'''
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  display: none; /* Hides all drop-down menus. */
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Encodes for DNA-binding protein(NR1), the activity of which depends on its phosphorylation. Drives expression of genes under GlnAp2 promoter
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                            Keep it sync with the drop-down menu width.
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}
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div.MenuBar ul li:hover ul.DropDownMenu li:hover ul.SideMenu li a,
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                            Use MenuTailor.css to customize. */
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  font: 12px arial, helvetica, sans-serif; /* Required for IE55. */
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  left: 13em; /* Places the side menu to the right of the drop-down menu.
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                            Keep it sync with the drop-down menu width.
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div.MenuBar ul li ul.DropDownMenu li ul.SideMenu li a span {
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  padding-left: 1.5em; /* Sets the left space of each side menu item. */
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  font: 12px arial, helvetica, sans-serif; /* Required for IE55. */
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  left: 13em; /* Places the side menu to the right of the drop-down menu.
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                            Keep it sync with the drop-down menu width.
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                            Use MenuTailor.css to customize. */
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/*-----------------------------------------------------------------------------------------------*/
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/*--------------------------------------------------------------------------------------- Widths */
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/*
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Menu Bar 1
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Side Menu #1
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}
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div.MenuBar#navi ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu#MB1-DDM2-SM2,
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div.MenuBar#navi ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu#MB1-DDM2-SM2 li a {
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  left: 15.5em  !important; /* Places the side menu to the right of the drop-down menu.
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                            Keep it sync with the drop-down menu width. */
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}
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div.MenuBar#navi ul li:hover ul.DropDownMenu li:hover ul.SideMenu#MB1-DDM2-SM3,
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div.MenuBar#navi ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu#MB1-DDM2-SM3,
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div.MenuBar#navi ul li:hover ul.DropDownMenu li:hover ul.SideMenu#MB1-DDM2-SM3 li a,
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div.MenuBar#navi ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu#MB1-DDM2-SM3 li a {
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</style>
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<div id="header"><img src=" https://static.igem.org/mediawiki/2009/a/a6/UQ_banner.jpg" /></div>
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<div class='MenuBar' id="navi">
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<ul>
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<li>
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<a href="https://2011.igem.org/Team:UQ-Australia" style="color: white">Home
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<!--[if lt IE 7]><table border="0" cellpadding="0" cellspacing="0"><tr><td><![endif]-->
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<!--[if lte IE 6]></td></tr></table></a><![endif]-->
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<li>
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<a href="https://2011.igem.org/Team:UQ-Australia/Team" style="color: white">Team
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                        <!--[if gt IE 6]><!--></a><!--<![endif]-->
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</li>
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                <li>
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<a href="https://2011.igem.org/Team:UQ-Australia/Project" style="color: white">Project
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                        <!--[if gt IE 6]><!--></a><!--<![endif]-->
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{|align="justify"
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|You can write a background of your team here.  Give us a background of your team, the members, etc. Or tell us more about something of your choosing.
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Encodes for a positive regulatory protein for L-arabinose utilization in E.coli
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DNA-binding transcriptional inhibitor for lac-operon
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===<span style="color:#558822"> Parts </span>===
 
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New for iGEM 2010 is the ''groupparts'' tag.  This tag will generate a table with all of the parts that your team adds to your team sandbox.  Note that if you want to document a part you need to document it on the [http://partsregistry.org Registry], not on your team wiki.
 
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<groupparts>iGEM010 UQ-Australia</groupparts>
 

Latest revision as of 03:58, 6 October 2011




The human circadian rhythm drives many important processes in the body in accordance with the sleep/wake cycle. A characteristic of this biological clock is the periodic oscillation of gene expression. Current parts in the Registry designed to regulate periodic oscillations of gene expression have shown limited success.

Here we demonstrate a biological clock being standardised as a set of BioBrick parts.

Our network is controlled by an engineered promoter, Plac/ara, which features both an activator and a repressor domain. This controls the production of downstream genes to activate other inducible promoters, pBAD and GlnAp2, eventually leading to the production of a repressor protein, lacI, which inhibits Plac/ara, resulting in oscillatory expression. This project shows the feasibility of standardising the biological clock in E. coli and grounds further development for applications in regulated drug/hormone delivery and ion channel control.

We were not completely successful in our experimental endeavours towards making the BioBrick parts to be submitted to the registry, however we were able to add BioBrick sites to each part listed below. Therefore, except for Plac/ara we had all the parts ready to be cloned into the iGEM submission vector pSB1C3. For more information on the laboratory work please see the Project page.

UQ-Australia logo 2011.png

Parts

MultiplePromoter image.png Plac/ara

A multi-domain promoter that is self-inducible by arabinose. Gene expression regulated by LacI binding to special operator sites in the promoter

Gene image.png glnG

Encodes for DNA-binding protein(NR1), the activity of which depends on its phosphorylation. Drives expression of genes under GlnAp2 promoter

Gene image.png araC

Encodes for a positive regulatory protein for L-arabinose utilization in E.coli

PositivePromoter.png pBAD

L-arabinose inducible promoter

Gene image.png LacI

DNA-binding transcriptional inhibitor for lac-operon

PositivePromoter.png GlnAp2

Promoter driven by phosphorylated GlnG(NR1)

Retrieved from "http://2011.igem.org/Team:UQ-Australia/Parts"