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| - | __NOTOC__
| + | {{:Team:UQ-Australia/Template:Header}} |
| - | <html>
| + | {|style="width:100%;" border="0" cellpadding="10" cellspacing="0" |
| - | <body style="background-color:#772288">
| + | |- valign="top" |
| | + | |rowspan="2"|A brief summary of some of our laboratory findings is included below. For more information, see the [[Team:UQ-Australia/Project|Project]] page. From these gels, you can see the amplification of our genes of interest, which we achieved with primers that added the standard BioBrick prefix and suffix to each end of the genes. As a result, these constructs are all ready for ligation and the construction of our oscillator modules! |
| | + | ![[File:UQ-Australia_logo_2011.png|125x125px|link=https://2011.igem.org/Team:UQ-Australia]] |
| | + | |- |
| | + | | |
| | + | |} |
| | | | |
| - | <style>
| + | We conducted a heat cycle PCR to determine the optimum conditions for amplifying our araC gene. The lanes here are loaded as follows: |
| - | h1.firstHeading { display: none; }
| + | |
| | | | |
| - | p {text-align: justify;}
| + | 1: 1kb+ ladder |
| | | | |
| - | a:link { color: #603371; text-decoration: none}
| + | 2: Negative control (H2O) |
| - | a:visited { color:#603371; text-decoration: none}
| + | |
| - | a:hover { color:#f29400; text-decoration: none}
| + | |
| - | a:active { color:#f29400; text-decoration: none}
| + | |
| | | | |
| - | #bodyContent { padding: 10px auto; width: 910px; margin: auto; clear: none; }
| + | 3-7: pBAD33 plasmid at temperatures 50C, 55C, 58C, 60C and 65C. |
| | | | |
| - | table#team_members { text-align: justify; border: 0; }
| + | 8: Negative control (H2O) |
| - | table#team_members h2, table#team_members h3 { clear: both; }
| + | |
| | | | |
| | + | 9-13: pBAD33 plasmid at temperatures 50C, 55C, 58C, 60C and 65C. |
| | | | |
| - | /*-----------------------------------------------------------------------------------------------*/
| + | The expected 900bp band for araC is seen at each temperature |
| - | div.MenuBar ul li ul.DropDownMenu {
| + | |
| - | display: none; /* Hides all drop-down menus. */
| + | |
| | | | |
| - | }
| + | [[File:AraC.jpg| 500x150px]] |
| - | /*
| + | |
| - | li:hover works in IE7 and FF2.
| + | |
| - | a:hover works in IE6 and FF2.
| + | |
| - | a:hover breaks li:hover in FF2.
| + | |
| - | */
| + | |
| - | div.MenuBar ul li:hover ul.DropDownMenu li ul.SideMenu,
| + | |
| - | div.MenuBar ul li a:hover ul.DropDownMenu li a ul.SideMenu {
| + | |
| - | display: none; /* Hides all side menus. */
| + | |
| - | }
| + | |
| - | /*------------------------------------------------------------------------------------- Menu Bar */
| + | |
| - | div.MenuBar {
| + | |
| - | font: arial, helvetica, sans-serif;
| + | |
| - | height: 30px;
| + | |
| - | width: 910px;
| + | |
| - | /*width: 100%*/
| + | |
| - | margin: 0;
| + | |
| - | border-top: 0;
| + | |
| - | border-right: 0;
| + | |
| - | border-left: 0;
| + | |
| - | padding: 0;
| + | |
| - | background: black;
| + | |
| - |
| + | |
| - | }
| + | |
| - | div.MenuBar ul {
| + | |
| - | font: arial, helvetica, sans-serif;
| + | |
| - | text-align: center;
| + | |
| - | list-style-type: none;
| + | |
| - | margin: 0.5em auto;
| + | |
| - | border: 0;
| + | |
| - | padding: 0;
| + | |
| - | background: black;
| + | |
| - | }
| + | |
| - | div.MenuBar ul li {
| + | |
| - | font: arial, helvetica, sans-serif;
| + | |
| - | display: block;
| + | |
| - | padding: 0;
| + | |
| - | margin: 0;
| + | |
| - | font-size: 1.3em;
| + | |
| - | float: left;
| + | |
| - | background: black;
| + | |
| - | text-align: center;
| + | |
| - | width: 107px;
| + | |
| - | position: relative; /* Sets the positioning context for each drop-down menu. */
| + | |
| - | }
| + | |
| | | | |
| - | div.MenuBar ul li a {
| + | PCR of the lacI gene showing the expected ~1kb band. |
| - | font: arial, helvetica, sans-serif;
| + | |
| - | display: block;
| + | |
| - | background: black;
| + | |
| - | height: 22px; /* Keep height + padding-top + padding-bottom sync with the menu bar height. */
| + | |
| - | color: #ffffff;
| + | |
| - | padding-top: 4px;
| + | |
| - | padding-bottom: 4px;
| + | |
| - | padding-left: 1em; /* Sets the left space between top-level items. */
| + | |
| - | padding-right: 1em; /* Sets the right space between top-level items. */
| + | |
| - | text-decoration: none;
| + | |
| - | }
| + | |
| | | | |
| - | /*------------------------------------------------------------------------------ Drop-Down Menus */
| + | [[File:Laci.jpg]] |
| - | div.MenuBar ul li:hover ul.DropDownMenu,
| + | |
| - | div.MenuBar ul li a:hover ul.DropDownMenu {
| + | |
| - | display: block;
| + | |
| - | width: 10em; /* Drop-down menu width.
| + | |
| - | Use MenuTailor.css to customize. */
| + | |
| - | height: 1em;
| + | |
| - | padding: 1px; /* Sets the drop-down menu "effective border" width. */
| + | |
| - | position: absolute;
| + | |
| - | top: 23px; /* Places the drop-down menu under the menu bar.
| + | |
| - | Keep it sync with the menu bar height. */
| + | |
| - | left: 0; /* Aligns the drop-down menu to its top-level item. */
| + | |
| - | background-color: black; /* Selected item. */
| + | |
| - | color: #FFFFFF;
| + | |
| | | | |
| - | }
| + | PCR of glnG (lanes 2 and 3), glnAp2 (lanes 5 and 6), and the promoter Plac/ara (lanes 2-4 of the second gel). Only glnG is the expected size (1.6kb), while the other two significantly smaller parts (200-300bp) have not been amplified. |
| - | div.MenuBar ul li:hover ul.DropDownMenu li a,
| + | |
| - | div.MenuBar ul li a:hover ul.DropDownMenu li a {
| + | |
| - | width: 10em; /* Keep it sync with the drop-down menu width.
| + | |
| - | Use MenuTailor.css to customize. */
| + | |
| - | height: 1em;
| + | |
| - | padding-left: 0;
| + | |
| - | padding-right: 0;
| + | |
| - | background-color: black; /* Selected item. */
| + | |
| - | color: #FFFFFF;
| + | |
| - | }
| + | |
| - | ul.DropDownMenu li a span {
| + | |
| - | display: block;
| + | |
| - | padding-left: 0.75em; /* Sets the left space of each drop-down menu item. */
| + | |
| - | padding-right: 0.25em; /* Sets the right space of each drop-down menu item. */
| + | |
| - | text-align: right; /* Aligns the >> symbol to the right. */
| + | |
| - | }
| + | |
| - | ul.DropDownMenu li a span span {
| + | |
| - | float: left; /* Aligns the text (back) to the left. */
| + | |
| - | font: 12px arial, helvetica, sans-serif; /* Required for IE55. */
| + | |
| - | height: 20px;
| + | |
| - | color: #FFFFFF;
| + | |
| - | }
| + | |
| - | /*----------------------------------------------------------------------------------- Side Menus */
| + | |
| - | div.MenuBar ul li:hover ul.DropDownMenu li:hover ul.SideMenu,
| + | |
| - | div.MenuBar ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu {
| + | |
| - | display: block;
| + | |
| - | width: 11em; /* Side menu width.
| + | |
| - | Use MenuTailor.css to customize. */
| + | |
| - | padding: 1px; /* Sets the side menu "effective border" width. */
| + | |
| - | position: absolute;
| + | |
| - | top: -1px; /* Aligns the side menu to its drop-down menu item.
| + | |
| - | Keep it sync with the side menu "effective border" width. */
| + | |
| - | left: 13em; /* Places the side menu to the right of the drop-down menu.
| + | |
| - | Keep it sync with the drop-down menu width.
| + | |
| - | Use MenuTailor.css to customize. */
| + | |
| - | }
| + | |
| - | div.MenuBar ul li:hover ul.DropDownMenu li:hover ul.SideMenu li a,
| + | |
| - | div.MenuBar ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu li a {
| + | |
| - | width: 11em; /* Keep it sync with the side menu width.
| + | |
| - | Use MenuTailor.css to customize. */
| + | |
| - | font: 12px arial, helvetica, sans-serif; /* Required for IE55. */
| + | |
| - | left: 13em; /* Places the side menu to the right of the drop-down menu.
| + | |
| - | Keep it sync with the drop-down menu width.
| + | |
| - | Use MenuTailor.css to customize. */
| + | |
| - | }
| + | |
| - | div.MenuBar ul li ul.DropDownMenu li ul.SideMenu li a span {
| + | |
| - | padding-left: 1.5em; /* Sets the left space of each side menu item. */
| + | |
| - | padding-right: 0.5em; /* Sets the right space of each side menu item. */
| + | |
| - | text-align: left;
| + | |
| - | font: 12px arial, helvetica, sans-serif; /* Required for IE55. */
| + | |
| - | left: 13em; /* Places the side menu to the right of the drop-down menu.
| + | |
| - | Keep it sync with the drop-down menu width.
| + | |
| - | Use MenuTailor.css to customize. */
| + | |
| - | }
| + | |
| - | /*----------------------------------------------------------------------------- Browser Specific */
| + | |
| - | * html div.MenuBar ul li a {
| + | |
| - | float: left; /* Required for IE55 and IE6.
| + | |
| - | Breaks O9.
| + | |
| - | Hidden (* html) from non-IE browsers. */
| + | |
| - | }
| + | |
| - | * html ul.DropDownMenu li a:hover {
| + | |
| - | cursor: hand; /* Required for IE55.
| + | |
| - | Hidden (* html) from non-IE browsers. */
| + | |
| - | }
| + | |
| - | ul.DropDownMenu li a:hover {
| + | |
| - | cursor: pointer; /* Required for IE6 and IE7.
| + | |
| - | Hidding it (* html) from non-IE browsers breaks IE7!
| + | |
| - | }
| + | |
| - | * html div.MenuBar a:hover {
| + | |
| - | text-decoration: none; /* Required for IE55 and IE6.
| + | |
| - | Hidden (* html) from non-IE browsers. */
| + | |
| - | }
| + | |
| - | * html div.MenuBar ul li table,
| + | |
| - | * html div.MenuBar ul li table td {
| + | |
| - | border: 0; /* Required for IE55 and IE6.
| + | |
| - | Hidden (* html) from non-IE browsers. */
| + | |
| - | }
| + | |
| - | /*------------------------------------------------------------------------------- Default Colors */
| + | |
| - | div.MenuBar {
| + | |
| - | background-color: Menu;
| + | |
| - | border-bottom: 1px solid ButtonShadow;
| + | |
| - | }
| + | |
| - | div.MenuBar a {
| + | |
| - | background-color: Menu; /* Top-level unselected items. */
| + | |
| - | color: MenuText;
| + | |
| - | }
| + | |
| - | div.MenuBar ul li:hover a,
| + | |
| - | div.MenuBar ul li a:hover {
| + | |
| - | color: #ea7f16;
| + | |
| - | background-color: Highlight; /* Top-level selected item. */
| + | |
| - | color: HighlightText;
| + | |
| - | }
| + | |
| - | /*...............................................................................................*/
| + | |
| - | div.MenuBar ul li:hover ul.DropDownMenu,
| + | |
| - | div.MenuBar ul li a:hover ul.DropDownMenu {
| + | |
| - | background-color: ButtonShadow; /* Sets the drop-down menu "effective border" color. */
| + | |
| - | }
| + | |
| - | div.MenuBar ul li:hover ul.DropDownMenu li a,
| + | |
| - | div.MenuBar ul li a:hover ul.DropDownMenu li a {
| + | |
| - | background-color: Menu; Drop-down menu unselected items.
| + | |
| - | Sets the drop-down menu "effective background" color. */
| + | |
| - | color: MenuText;
| + | |
| - | }
| + | |
| - | div.MenuBar ul li:hover ul.DropDownMenu li:hover a,
| + | |
| - | div.MenuBar ul li a:hover ul.DropDownMenu li a:hover {
| + | |
| - | background-color: Highlight; /* Drop-down menu selected item. */
| + | |
| - | color: HighlightText;
| + | |
| - | }
| + | |
| - | /*...............................................................................................*/
| + | |
| - | div.MenuBar ul li:hover ul.DropDownMenu li:hover ul.SideMenu,
| + | |
| - | div.MenuBar ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu {
| + | |
| - | background-color: ButtonShadow; /* Sets the side menu "effective border" color. */
| + | |
| - | }
| + | |
| - | div.MenuBar ul li:hover ul.DropDownMenu li:hover ul.SideMenu li a,
| + | |
| - | div.MenuBar ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu li a {
| + | |
| - | background-color: Menu; /* Side menu unselected items.
| + | |
| - | Sets the side menu "effective background" color. */
| + | |
| - | color: MenuText;
| + | |
| - | }
| + | |
| - | div.MenuBar ul li:hover ul.DropDownMenu li:hover ul.SideMenu li a:hover,
| + | |
| - | div.MenuBar ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu li a:hover {
| + | |
| - | background-color: Highlight; /* Side menu selected item. */
| + | |
| - | color: HighlightText;
| + | |
| - | }
| + | |
| - | /*-----------------------------------------------------------------------------------------------*/
| + | |
| - | /*Script-Free 3-Level Menu 1.2 Tailor
| + | |
| - | www.CesarDaniel.info
| + | |
| - | /*-------------------------------------------------------------------------------------- General */
| + | |
| - | body {
| + | |
| - | background: white;
| + | |
| - | color: black;
| + | |
| - | margin: 0;
| + | |
| - | border: 0;
| + | |
| - | padding: 0;
| + | |
| - | }
| + | |
| | | | |
| - | | + | [[File:Glngplac.jpg | 600x400px]] |
| - | div.MenuBar {
| + | |
| - | font: 13px arial, helvetica, sans-serif;
| + | |
| - | }
| + | |
| - | div.MenuBar ul {
| + | |
| - | font: 13px arial, helvetica, sans-serif; /* Required for IE55. */
| + | |
| - | }
| + | |
| - | /*--------------------------------------------------------------------------------------- Colors */
| + | |
| - | div.MenuBar {
| + | |
| - | background-color: black; /* Selected item. */
| + | |
| - | color: #FFFFFF;
| + | |
| - | border-bottom: 1px solid ButtonShadow;
| + | |
| - | }
| + | |
| - | div.MenuBar a,
| + | |
| - | div.MenuBar ul li:hover ul.DropDownMenu li a,
| + | |
| - | div.MenuBar ul li a:hover ul.DropDownMenu li a,
| + | |
| - | div.MenuBar ul li:hover ul.DropDownMenu li:hover ul.SideMenu li a,
| + | |
| - | div.MenuBar ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu li a {
| + | |
| - | background-color: black; /* Selected item. */
| + | |
| - | color: #FFFFFF;
| + | |
| - | }
| + | |
| - | div.MenuBar ul li:hover a,
| + | |
| - | div.MenuBar ul li a:hover,
| + | |
| - | div.MenuBar ul li:hover ul.DropDownMenu li:hover a,
| + | |
| - | div.MenuBar ul li a:hover ul.DropDownMenu li a:hover,
| + | |
| - | div.MenuBar ul li:hover ul.DropDownMenu li:hover ul.SideMenu li a:hover,
| + | |
| - | div.MenuBar ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu li a:hover {
| + | |
| - | background-color: #603371; /* Selected item. */
| + | |
| - | color: #FFFFFF;
| + | |
| - | }
| + | |
| - | div.MenuBar ul li:hover ul.DropDownMenu,
| + | |
| - | div.MenuBar ul li a:hover ul.DropDownMenu,
| + | |
| - | div.MenuBar ul li:hover ul.DropDownMenu li:hover ul.SideMenu,
| + | |
| - | div.MenuBar ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu {
| + | |
| - | background-color: ButtonShadow; /* Sets the drop-down and side menus "effective border" color. */
| + | |
| - | }
| + | |
| - | /*--------------------------------------------------------------------------------------- Widths */
| + | |
| - | /*
| + | |
| - | | + | |
| - | /*
| + | |
| - | Menu Bar 1
| + | |
| - | Drop-Down Menu #2
| + | |
| - | */
| + | |
| - | div.MenuBar#navi ul li:hover ul.DropDownMenu#MB1-DDM4,
| + | |
| - | div.MenuBar#navi ul li a:hover ul.DropDownMenu#MB1-DDM4,
| + | |
| - | div.MenuBar#navi ul li:hover ul.DropDownMenu#MB1-DDM4 li a,
| + | |
| - | div.MenuBar#navi ul li a:hover ul.DropDownMenu#MB1-DDM4 li a {
| + | |
| - | width: 11em; /* Drop-down menu width. */
| + | |
| - | }
| + | |
| - | div.MenuBar#navi ul li:hover ul.DropDownMenu#MB1-DDM5,
| + | |
| - | div.MenuBar#navi ul li a:hover ul.DropDownMenu#MB1-DDM5,
| + | |
| - | div.MenuBar#navi ul li:hover ul.DropDownMenu#MB1-DDM5 li a,
| + | |
| - | div.MenuBar#navi ul li a:hover ul.DropDownMenu#MB1-DDM5 li a {
| + | |
| - | width: 12em; /* Drop-down menu width. */
| + | |
| - | }
| + | |
| - | | + | |
| - | /*...............................................................................................*/
| + | |
| - | /*
| + | |
| - | Menu Bar 1
| + | |
| - | Drop-Down Menu #2
| + | |
| - | Side Menu #1
| + | |
| - | */
| + | |
| - | div.MenuBar#navi ul li:hover ul.DropDownMenu li:hover ul.SideMenu#MB1-DDM2-SM1,
| + | |
| - | div.MenuBar#navi ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu#MB1-DDM2-SM1 {
| + | |
| - | left: 15.5em !important; /* Places the side menu to the right of the drop-down menu.
| + | |
| - | Keep it sync with the drop-down menu width. */
| + | |
| - | }
| + | |
| - | div.MenuBar#navi ul li:hover ul.DropDownMenu li:hover ul.SideMenu#MB1-DDM2-SM1,
| + | |
| - | div.MenuBar#navi ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu#MB1-DDM2-SM1,
| + | |
| - | div.MenuBar#navi ul li:hover ul.DropDownMenu li:hover ul.SideMenu#MB1-DDM2-SM1 li a,
| + | |
| - | div.MenuBar#navi ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu#MB1-DDM2-SM1 li a {
| + | |
| - | width: 10em; /* Side menu width. */
| + | |
| - | }
| + | |
| - | /*...............................................................................................*/
| + | |
| - | /*
| + | |
| - | Menu Bar 1
| + | |
| - | Drop-Down Menu #2
| + | |
| - | Side Menu #2
| + | |
| - | */
| + | |
| - | div.MenuBar#navi ul li:hover ul.DropDownMenu li:hover ul.SideMenu#MB1-DDM2-SM2,
| + | |
| - | div.MenuBar#navi ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu#MB1-DDM2-SM2 {
| + | |
| - | left: 15.5em !important; /* Places the side menu to the right of the drop-down menu.
| + | |
| - | Keep it sync with the drop-down menu width. */
| + | |
| - | }
| + | |
| - | div.MenuBar#navi ul li:hover ul.DropDownMenu li:hover ul.SideMenu#MB1-DDM2-SM2,
| + | |
| - | div.MenuBar#navi ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu#MB1-DDM2-SM2,
| + | |
| - | div.MenuBar#navi ul li:hover ul.DropDownMenu li:hover ul.SideMenu#MB1-DDM2-SM2 li a,
| + | |
| - | div.MenuBar#navi ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu#MB1-DDM2-SM2 li a {
| + | |
| - | width: 10em; /* Side menu width. */
| + | |
| - | }
| + | |
| - | /*...............................................................................................*/
| + | |
| - | /*
| + | |
| - | Menu Bar 1
| + | |
| - | Drop-Down Menu #2
| + | |
| - | Side Menu #3
| + | |
| - | */
| + | |
| - | div.MenuBar#navi ul li:hover ul.DropDownMenu li:hover ul.SideMenu#MB1-DDM2-SM3,
| + | |
| - | div.MenuBar#navi ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu#MB1-DDM2-SM3 {
| + | |
| - | left: 15.5em !important; /* Places the side menu to the right of the drop-down menu.
| + | |
| - | Keep it sync with the drop-down menu width. */
| + | |
| - | }
| + | |
| - | div.MenuBar#navi ul li:hover ul.DropDownMenu li:hover ul.SideMenu#MB1-DDM2-SM3,
| + | |
| - | div.MenuBar#navi ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu#MB1-DDM2-SM3,
| + | |
| - | div.MenuBar#navi ul li:hover ul.DropDownMenu li:hover ul.SideMenu#MB1-DDM2-SM3 li a,
| + | |
| - | div.MenuBar#navi ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu#MB1-DDM2-SM3 li a {
| + | |
| - | width: 10em; /* Side menu width. */
| + | |
| - | }
| + | |
| - | /*...............................................................................................*/
| + | |
| - | | + | |
| - | </style>
| + | |
| - | | + | |
| - | | + | |
| - | <body>
| + | |
| - | <div id="header"><img src=" https://static.igem.org/mediawiki/2009/a/a6/UQ_banner.jpg" /></div>
| + | |
| - | <div class='MenuBar' id="navi">
| + | |
| - | <ul>
| + | |
| - | <li>
| + | |
| - | <a href="https://2009.igem.org/Team:UQ-Australia" style="color: white">Home
| + | |
| - | <!--[if gt IE 6]><!--></a><!--<![endif]-->
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| - | <a href="https://2009.igem.org/Team:UQ-Australia/Team" style="color: white">Team
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| - | <a href="https://2009.igem.org/Team:UQ-Australia/Project" style="color: white">Projects
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| - | <a href="https://2009.igem.org/Team:UQ-Australia/Parts" style="color: white">Parts
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| - | <a href="https://2009.igem.org/Team:UQ-Australia/Model" style="color:white">Modelling
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| - | <a href="https://2009.igem.org/Team:UQ-Australia/Notebook" style="color: white">Notebook
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| - | <a href="https://2009.igem.org/Team:UQ-Australia/Lab" style="color: white">Our Lab
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| - | <a href="https://2009.igem.org/Team:UQ-Australia/Sponsors" style="color: white">Sponsors</a>
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| - | </ul>
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