Team:UQ-Australia

From 2011.igem.org

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|rowspan="2"|The human circadian rhythm drives many important processes in the body in accordance with the sleep/wake cycle. A characteristic of this biological clock is the periodic oscillation of gene expression. Current parts in the Registry designed to regulate periodic oscillations of gene expression have shown limited success.
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Here we demonstrate the feasibility a biological clock being standardised as a set of BioBrick parts.
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__NOTOC__
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Our network is controlled by an engineered promoter, Plac/ara, which features both an activator and a repressor domain. This controls the production of downstream genes to activate other inducible promoters, pBAD and GlnAp2, eventually leading to the production of a repressor protein, lacI, which inhibits Plac/ara, resulting in oscillatory expression. This project shows the feasibility of standardising the biological clock in E. coli and grounds further development for applications in regulated drug/hormone delivery and ion channel control.
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<ul>
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<li>
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<a href="https://2011.igem.org/Team:UQ-Australia" style="color: white">Home
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<!--[if gt IE 6]><!--></a><!--<![endif]-->
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<a href="https://2011.igem.org/Team:UQ-Australia/Team" style="color: white">Team
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<a href="https://2011.igem.org/Team:UQ-Australia/Project" style="color: white">Project
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                        <!--[if gt IE 6]><!--></a><!--<![endif]-->
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                  </li>
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    <li>
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<a href="https://2011.igem.org/Team:UQ-Australia/Parts" style="color: white">Parts
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                        <!--[if gt IE 6]><!--></a><!--<![endif]-->
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<!--[if lt IE 7]><table border="0" cellpadding="0" cellspacing="0"><tr><td><![endif]-->
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</li>
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<a href="https://2011.igem.org/Team:UQ-Australia/Data" style="color: white">Data
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We are the iGEM Team from the University of Queensland, Brisbane, Australia.
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On time, ready for the jamboree!
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|[[Image:UQ-Australia_logo.png|200px|right|frame]]
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|[[File:UQ-Australia_logo_2011.png|125x125px|link=https://2011.igem.org/Team:UQ-Australia]]
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''During the year we will be working with researchers from a diverse range of disciplines to create our own genetically engineered machine.''
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Inspired by the circadian clock in humans which regulates a number of very important processes, we are trying to replicate this biological clock in a bacterial system. We are aiming to construct a network of genes that oscillates in a similar fashion to the 24 hour system in humans. If we are successful, we will be able to put different genes into our system so that we can make the bacteria perform a particular process periodically – a simple example of this would be to make them flash on and off consistently.
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To achieve this oscillatory behaviour we will utilise a gene network with a series of inducible promoters that generate the production of other activating proteins, all driven by a constitutively active promoter. This promoter features an engineered repression domain (the inhibitor of this promoter being the output of the final step in the network). If everything goes as planned, these linked activations and repression will produce fluctuating levels of the proteins in question, which could then be used to drive our output function (initially just GFP production and a timed fluorescence). Ultimately, we hope our system could be used to drive the timed release of drugs or other biological factors.
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Find us on [http://www.facebook.com/pages/UQ-iGEM-2011/174356539258535 Facebook]!
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|[[Image:UQ-Australia_team.png|right|frame|Your team picture]]
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|align="center"|[[Team:UQ-Australia | Team Example]]
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'''NB: wiki best viewed on Chrome, Firefox or Safari.'''
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Latest revision as of 00:32, 6 October 2011




The human circadian rhythm drives many important processes in the body in accordance with the sleep/wake cycle. A characteristic of this biological clock is the periodic oscillation of gene expression. Current parts in the Registry designed to regulate periodic oscillations of gene expression have shown limited success.

Here we demonstrate the feasibility a biological clock being standardised as a set of BioBrick parts.

Our network is controlled by an engineered promoter, Plac/ara, which features both an activator and a repressor domain. This controls the production of downstream genes to activate other inducible promoters, pBAD and GlnAp2, eventually leading to the production of a repressor protein, lacI, which inhibits Plac/ara, resulting in oscillatory expression. This project shows the feasibility of standardising the biological clock in E. coli and grounds further development for applications in regulated drug/hormone delivery and ion channel control.

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UQ-Australia logo 2011.png


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