Team:Tokyo Metropolitan

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</html><html><div align="center"><a href="https://2011.igem.org/Team:Tokyo_Metropolitan" border="0"><img src="https://static.igem.org/mediawiki/2011/5/5e/Tokyo_Metropolitan_Header.png"></a><br>
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<font size="4">Welcome!</font><hr>
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　This is team Tokyo_Metropolitan 2011 wiki. We have a project "BeE.coli" on iGEM 2011 and tackled safety issues or human practice proposal. Please click menu to see more our pages!
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  <br>
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<td align="center" valign="bottom"><a href="http://twitter.com/#!/igemtmu">
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<img src="https://static.igem.org/mediawiki/2011/d/d5/Tokyo_Metropolitan_twitter.png" height="80">
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<br><font size="1">Follow us on twitter!</font></a></td>
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<td width="5"></td>
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<td align="center" valign="bottom"><a href="https://2011.igem.org/Main_Page">
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<img src="https://static.igem.org/mediawiki/2011/6/62/Tokyo_Metropolitan_igemlogo.png" height="80">
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<br><font size="1">Go to iGEM 2011 Main Page</font></a></td>
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<font size="4">Abstract : BeE.coli</font>
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　Team Tokyo_Metropolitan 2011 propose the project "BeE.coli" as a micro injection system.BeE.coli has the abilities to move fast and target pathogens concentrated area.Getting close to pathogens, it sends the killer gene to them with conjugation. Then pathogens will be killed.<br>
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　To accomplish this project, we planned to add three functions to E.coli. First, expression of the mutant H-NS proteins enables E.coli to move faster. This contributes to increasing the frequency of conjugation.<br>
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　Second function is new taxis for AHL. We make the target bacteria as a model of pathogens, and they produce AHL. BeE.coli goes for the area where high concentrations of AHL that means concentration of target bacteria.<br>
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　Last, BeE.coli kills pathogens by sending the killer gene with the conjugative plasmid. BeE.coli has the anti killer gene repressing the killer gene.<br>
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　In the future, BeE.coli will be useful for medical scenes.
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Taxis for AHL!<br>
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BeE.coli go to area where target bacteria is concentrated.<br>
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<a href="https://2011.igem.org/Team:Tokyo_Metropolitan/Project/Targeting">⇒See detail.</a>
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<font size="4"><b>Our project : BeE.coli</b></font><br><br>
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We propose the project "BeE.coli" as a micro injection system. <br>
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<table border="0"><tr height="83"><td width="440"></td><td width="210">
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BeE.coli has the abilities to move fast and target pathogens concentrated area.  <br>
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<div align="center">
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Getting close to pathogens, it sends the killer gene to them with conjugation. Then pathogens will be killed.<br>
+
Get high speed move!<br>
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<br>
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BeE.coli have mutant HNS protein(T108I).<br>
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To accomplish this project, we planned to add three functions to E.coli.<br>
+
<a href="https://2011.igem.org/Team:Tokyo_Metropolitan/Project/SpeedUp">⇒See details</a>
-
First, expression of the mutant H-NS proteins enables E.coli to move faster. This contributes to increasing the frequency of conjugation.<br>
+
-
Second function is new taxis for AHL. We make the target bacteria as a model of pathogens, and they produce AHL. BeE.coli goes for the area where high concentrations of AHL that means concentration of target bacteria. <br>
+
-
Last, BeE.coli kills pathogens by sending the killer gene with the conjugative plasmid. BeE.coli has the anti killer gene repressing the killer gene.<br>
+
-
<br>
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In the future, BeE.coli will be useful for medical scenes.<br>
+
</div>
</div>
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</td></tr></table>
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<tr height="40"><td width="650"></td></tr>
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<tr height="87"><td>
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<table border="0"><tr height="83"><td width="325"></td><td width="220">
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<div align="center">
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Sting pathogens!<br>
 +
BeE.coli send holin gene to target bacteria with conjugation.<br>
 +
<a href="https://2011.igem.org/Team:Tokyo_Metropolitan/Project/Killing">⇒See detail</a>
 +
</div>
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<img src="http://igemtmu.net/2011/wiki/speedup.png" width="200">
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<img src="http://igemtmu.net/2011/wiki/targeting.png" width="200">
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<img src="http://igemtmu.net/2011/wiki/killing.png" width="200">
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<table><tr><td width="450" align="center"><b>Photo Gallery</b><br><embed type="application/x-shockwave-flash" src="https://picasaweb.google.com/s/c/bin/slideshow.swf" width="400" height="267" flashvars="host=picasaweb.google.com&hl=en_US&feat=flashalbum&RGB=0x000000&feed=https%3A%2F%2Fpicasaweb.google.com%2Fdata%2Ffeed%2Fapi%2Fuser%2FigemTMU%2Falbumid%2F5659929053369567921%3Falt%3Drss%26kind%3Dphoto%26hl%3Den_US" pluginspage="http://www.macromedia.com/go/getflashplayer"></embed></td><td width="450" align="center"><b>Our creation! Safety Promotion Video</b><br><br><iframe width="400" height="267" src="http://www.youtube.com/embed/JZwjYAyRaWk?hl=ja&fs=1" frameborder="0" allowfullscreen></iframe><Br><a href="https://2011.igem.org/Team:Tokyo_Metropolitan/Safety">⇒Click to see safety page.</a></td></tr><table>
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Latest revision as of 21:18, 5 October 2011
Welcome!
　This is team Tokyo_Metropolitan 2011 wiki. We have a project "BeE.coli" on iGEM 2011 and tackled safety issues or human practice proposal. Please click menu to see more our pages!

Follow us on twitter!
Go to iGEM 2011 Main Page
Abstract : BeE.coli
　Team Tokyo_Metropolitan 2011 propose the project "BeE.coli" as a micro injection system.BeE.coli has the abilities to move fast and target pathogens concentrated area.Getting close to pathogens, it sends the killer gene to them with conjugation. Then pathogens will be killed.
　To accomplish this project, we planned to add three functions to E.coli. First, expression of the mutant H-NS proteins enables E.coli to move faster. This contributes to increasing the frequency of conjugation.
　Second function is new taxis for AHL. We make the target bacteria as a model of pathogens, and they produce AHL. BeE.coli goes for the area where high concentrations of AHL that means concentration of target bacteria.
　Last, BeE.coli kills pathogens by sending the killer gene with the conjugative plasmid. BeE.coli has the anti killer gene repressing the killer gene.
　In the future, BeE.coli will be useful for medical scenes.
Taxis for AHL!
BeE.coli go to area where target bacteria is concentrated.
⇒See detail.
Get high speed move!
BeE.coli have mutant HNS protein(T108I).
⇒See details
Sting pathogens!
BeE.coli send holin gene to target bacteria with conjugation.
⇒See detail
Photo Gallery
Our creation! Safety Promotion Video


⇒Click to see safety page.

Retrieved from "http://2011.igem.org/Team:Tokyo_Metropolitan"