Team:Hong Kong-CUHK/Project/overview

Project Overview

Halorhodopsin, a light-driven ion pump originating from Halobacterium, employs light to transport chloride ions into cells uni-directionally against osmolality. Since chloride ion is a ubiquitous and essential element in most biological systems, halorhodopsin has the fascinating property to transform solar energy, the most abundant energy in the world, into intracellular chloride ion level. However, this specificity of halorhodopsin has not been well regulated and characterized in the previous competition. It is of our great interest to characterize its properties, and eventually utilize them in our project, seeking the possibilities to benefit synthetic biology and the human society.

In our project, we integrated halorhodopsin into functional biobricks. Based on the characterized properties of halorhodopsin, we have furthermore developed and implemented two innovative applications: light-mediated electricity generation and light-coupled computer-aided expression platform. The palatable results show us the power and the potential usage of this fascinating tool. We are glad to introduce our achievements in the project session.

Besides the proof-of-concept designs in our projects, any design with the linkage of light and chloride ions can be accomplished by this tool. Thus, there are much more potential applications development. We believe that halorhodopsin would be a promising and useful biobrick in the future.