Team:NYMU-Taipei/results/chassis1

From 2011.igem.org

(Difference between revisions)

Revision as of 19:06, 5 October 2011 (view source) Lchieh72 (Talk | contribs) ← Older edit		Revision as of 19:18, 5 October 2011 (view source) NickLai (Talk | contribs) Newer edit →
Line 11:		Line 11:
-	<font size=3>We present AMB-1's growth curve in terms of OD565, the maximum absorbance of AMB-1. It is important to find out the '''log phase''', because log phase is the best time for electroporation. We better keep AMB-1 in "log phase", because this phase is the index to do electroporation. The success rate will be greatly improved in log phase. Therefore, by keeping AMB-1 in "log phase" (approximately <font color=blue>'''3~6 days'''</font>), we can get the best results from electroporation. After seven days, the bacterium would be into '''stationary phase'''. During the phase, the growth rate slows as a result of nutrient depletion and accumulation of toxic products. This phase is reached as the bacteria begin to exhaust the resources that are available to them. This phase is a constant value as the rate of bacterial growth is equal to the rate of bacterial death. So, if we want to get better quality of electroporation. we'd better make sure there are enough AMB-1 strain in log phase.	+	<font size=3>We present AMB-1's growth curve in terms of OD565, the maximum absorbance of AMB-1. It is important to find out the '''log phase''', because log phase is the best time for electroporation. We better keep AMB-1 in "log phase", because this phase is the index to do electroporation. The success rate will be greatly improved in log phase. Therefore, by keeping AMB-1 in "log phase" (approximately <font color=blue>'''3~6 days'''</font> in Enrich-MSGM, <font color=blue>'''4~8 days'''</font> in MSGM), we can get the best results from electroporation. After seven days, the bacterium would be into '''stationary phase'''. During the phase, the growth rate slows as a result of nutrient depletion and accumulation of toxic products. This phase is reached as the bacteria begin to exhaust the resources that are available to them. This phase is a constant value as the rate of bacterial growth is equal to the rate of bacterial death. So, if we want to get better quality of electroporation. we'd better make sure there are enough AMB-1 strain in log phase.
	</font>		</font>

---

Revision as of 19:18, 5 October 2011

• Home Get to HOME
• Idea Our Idea
  Concept Brainstorming Background Review
• Design/ Experiment
  Optomagnetic Design Protein Modelling NeuroSymbiosis Chassis SynthoPrime Lab Protocol
• Results
  Optomagnetic Bacteria NeuroSymbiosis Chassis Parts International Collaboration
• Human Practice & Safety
  Synthetic Neurobiology Human Practice Evaluation & Safety Assessment
• About Us
  The School The Team China Meetup Acknowledgement

We present AMB-1's growth curve in terms of OD565, the maximum absorbance of AMB-1. It is important to find out the log phase, because log phase is the best time for electroporation. We better keep AMB-1 in "log phase", because this phase is the index to do electroporation. The success rate will be greatly improved in log phase. Therefore, by keeping AMB-1 in "log phase" (approximately 3~6 days in Enrich-MSGM, 4~8 days in MSGM), we can get the best results from electroporation. After seven days, the bacterium would be into stationary phase. During the phase, the growth rate slows as a result of nutrient depletion and accumulation of toxic products. This phase is reached as the bacteria begin to exhaust the resources that are available to them. This phase is a constant value as the rate of bacterial growth is equal to the rate of bacterial death. So, if we want to get better quality of electroporation. we'd better make sure there are enough AMB-1 strain in log phase.